Article: Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong
| Title | Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong |
|---|---|
| Authors | Ho, PL1 Lo, WU1 Yeung, MK1 Lin, CH1 Chow, KH1 Ang, I1 Tong, AHY1 BaoJessie, J YJ1 Lok, S1 Lo, JYC2 |
| Issue Date | 2011 |
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
| Citation | Plos One, 2011, v. 6 n. 3 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0017989 |
| Abstract | Background: The emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to virtually all β-lactam antibiotics and there is often co-resistance to other antibiotic classes, the therapeutic options for infections caused by these organisms are very limited. Methodology: We characterized the first NDM-1 producing E. coli isolate recovered in Hong Kong. The plasmid encoding the metallo-β-lactamase gene was sequenced. Principal Findings: The plasmid, pNDM-HK readily transferred to E. coli J53 at high frequencies. It belongs to the broad host range IncL/M incompatibility group and is 88803 bp in size. Sequence alignment showed that pNDM-HK has a 55 kb backbone which shared 97% homology with pEL60 originating from the plant pathogen, Erwina amylovora in Lebanon and a 28.9 kb variable region. The plasmid backbone includes the mucAB genes mediating ultraviolet light resistance. The 28.9 kb region has a composite transposon-like structure which includes intact or truncated genes associated with resistance to β-lactams (bla TEM-1, bla NDM-1, Δbla DHA-1), aminoglycosides (aacC2, armA), sulphonamides (sul1) and macrolides (mel, mph2). It also harbors the following mobile elements: IS26, ISCR1, tnpU, tnpAcp2, tnpD, ΔtnpATn1 and insL. Certain blocks within the 28.9 kb variable region had homology with the corresponding sequences in the widely disseminated plasmids, pCTX-M3, pMUR050 and pKP048 originating from bacteria in Poland in 1996, in Spain in 2002 and in China in 2006, respectively. Significance: The genetic support of NDM-1 gene suggests that it has evolved through complex pathways. The association with broad host range plasmid and multiple mobile genetic elements explain its observed horizontal mobility in multiple bacterial taxa. © 2011 Ho et al. |
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 |
| DOI | http://dx.doi.org/10.1371/journal.pone.0017989 |
| PubMed Central ID | PMC3061923 |
| References | References in Scopus |
| dc.contributor.author | Ho, PL | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Lo, WU | ||||
| dc.contributor.author | Yeung, MK | ||||
| dc.contributor.author | Lin, CH | ||||
| dc.contributor.author | Chow, KH | ||||
| dc.contributor.author | Ang, I | ||||
| dc.contributor.author | Tong, AHY | ||||
| dc.contributor.author | BaoJessie, J YJ | ||||
| dc.contributor.author | Lok, S | ||||
| dc.contributor.author | Lo, JYC | ||||
| dc.date.accessioned | 2011-08-26T14:24:52Z | ||||
| dc.date.available | 2011-08-26T14:24:52Z | ||||
| dc.date.issued | 2011 | ||||
| dc.description.abstract | Background: The emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to virtually all β-lactam antibiotics and there is often co-resistance to other antibiotic classes, the therapeutic options for infections caused by these organisms are very limited. Methodology: We characterized the first NDM-1 producing E. coli isolate recovered in Hong Kong. The plasmid encoding the metallo-β-lactamase gene was sequenced. Principal Findings: The plasmid, pNDM-HK readily transferred to E. coli J53 at high frequencies. It belongs to the broad host range IncL/M incompatibility group and is 88803 bp in size. Sequence alignment showed that pNDM-HK has a 55 kb backbone which shared 97% homology with pEL60 originating from the plant pathogen, Erwina amylovora in Lebanon and a 28.9 kb variable region. The plasmid backbone includes the mucAB genes mediating ultraviolet light resistance. The 28.9 kb region has a composite transposon-like structure which includes intact or truncated genes associated with resistance to β-lactams (bla TEM-1, bla NDM-1, Δbla DHA-1), aminoglycosides (aacC2, armA), sulphonamides (sul1) and macrolides (mel, mph2). It also harbors the following mobile elements: IS26, ISCR1, tnpU, tnpAcp2, tnpD, ΔtnpATn1 and insL. Certain blocks within the 28.9 kb variable region had homology with the corresponding sequences in the widely disseminated plasmids, pCTX-M3, pMUR050 and pKP048 originating from bacteria in Poland in 1996, in Spain in 2002 and in China in 2006, respectively. Significance: The genetic support of NDM-1 gene suggests that it has evolved through complex pathways. The association with broad host range plasmid and multiple mobile genetic elements explain its observed horizontal mobility in multiple bacterial taxa. © 2011 Ho et al. | ||||
| dc.description.nature | published_or_final_version | ||||
| dc.identifier.citation | Plos One, 2011, v. 6 n. 3 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0017989 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0017989 | ||||
| dc.identifier.epage | e17989 | ||||
| dc.identifier.hkuros | 191834 | ||||
| dc.identifier.isi | WOS:000288613300025
Funding Information: This work was supported by a commission grant from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Hong Kong, Special Administrative Region government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||
| dc.identifier.issue | 3 | ||||
| dc.identifier.pmcid | PMC3061923 | ||||
| dc.identifier.pmid | 21445317 | ||||
| dc.identifier.scopus | eid_2-s2.0-79952838766 | ||||
| dc.identifier.spage | e17989 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/137433 | ||||
| dc.identifier.volume | 6 | ||||
| dc.language | eng | ||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | PLoS ONE | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||
| dc.subject.mesh | Bacterial Proteins - genetics | ||||
| dc.subject.mesh | Escherichia coli - enzymology | ||||
| dc.subject.mesh | Hong Kong | ||||
| dc.subject.mesh | Plasmids | ||||
| dc.subject.mesh | beta-Lactamases - genetics | ||||
| dc.title | Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Centre for Health Protection