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Article: Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong

TitleComplete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong
Authors
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2011, v. 6 n. 3, article no. e17989 How to Cite?
Abstract
Background: The emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to virtually all β-lactam antibiotics and there is often co-resistance to other antibiotic classes, the therapeutic options for infections caused by these organisms are very limited. Methodology: We characterized the first NDM-1 producing E. coli isolate recovered in Hong Kong. The plasmid encoding the metallo-β-lactamase gene was sequenced. Principal Findings: The plasmid, pNDM-HK readily transferred to E. coli J53 at high frequencies. It belongs to the broad host range IncL/M incompatibility group and is 88803 bp in size. Sequence alignment showed that pNDM-HK has a 55 kb backbone which shared 97% homology with pEL60 originating from the plant pathogen, Erwina amylovora in Lebanon and a 28.9 kb variable region. The plasmid backbone includes the mucAB genes mediating ultraviolet light resistance. The 28.9 kb region has a composite transposon-like structure which includes intact or truncated genes associated with resistance to β-lactams (bla TEM-1, bla NDM-1, Δbla DHA-1), aminoglycosides (aacC2, armA), sulphonamides (sul1) and macrolides (mel, mph2). It also harbors the following mobile elements: IS26, ISCR1, tnpU, tnpAcp2, tnpD, ΔtnpATn1 and insL. Certain blocks within the 28.9 kb variable region had homology with the corresponding sequences in the widely disseminated plasmids, pCTX-M3, pMUR050 and pKP048 originating from bacteria in Poland in 1996, in Spain in 2002 and in China in 2006, respectively. Significance: The genetic support of NDM-1 gene suggests that it has evolved through complex pathways. The association with broad host range plasmid and multiple mobile genetic elements explain its observed horizontal mobility in multiple bacterial taxa. © 2011 Ho et al.
Persistent Identifierhttp://hdl.handle.net/10722/137433
ISSN
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Health and Food Bureau of the Hong Kong, Special Administrative Region government
Funding Information:

This work was supported by a commission grant from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Hong Kong, Special Administrative Region government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLo, WUen_HK
dc.contributor.authorYeung, MKen_HK
dc.contributor.authorLin, CHen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorAng, Ien_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorBao, JYJen_HK
dc.contributor.authorLok, Sen_HK
dc.contributor.authorLo, JYCen_HK
dc.date.accessioned2011-08-26T14:24:52Z-
dc.date.available2011-08-26T14:24:52Z-
dc.date.issued2011en_HK
dc.identifier.citationPlos One, 2011, v. 6 n. 3, article no. e17989en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137433-
dc.description.abstractBackground: The emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to virtually all β-lactam antibiotics and there is often co-resistance to other antibiotic classes, the therapeutic options for infections caused by these organisms are very limited. Methodology: We characterized the first NDM-1 producing E. coli isolate recovered in Hong Kong. The plasmid encoding the metallo-β-lactamase gene was sequenced. Principal Findings: The plasmid, pNDM-HK readily transferred to E. coli J53 at high frequencies. It belongs to the broad host range IncL/M incompatibility group and is 88803 bp in size. Sequence alignment showed that pNDM-HK has a 55 kb backbone which shared 97% homology with pEL60 originating from the plant pathogen, Erwina amylovora in Lebanon and a 28.9 kb variable region. The plasmid backbone includes the mucAB genes mediating ultraviolet light resistance. The 28.9 kb region has a composite transposon-like structure which includes intact or truncated genes associated with resistance to β-lactams (bla TEM-1, bla NDM-1, Δbla DHA-1), aminoglycosides (aacC2, armA), sulphonamides (sul1) and macrolides (mel, mph2). It also harbors the following mobile elements: IS26, ISCR1, tnpU, tnpAcp2, tnpD, ΔtnpATn1 and insL. Certain blocks within the 28.9 kb variable region had homology with the corresponding sequences in the widely disseminated plasmids, pCTX-M3, pMUR050 and pKP048 originating from bacteria in Poland in 1996, in Spain in 2002 and in China in 2006, respectively. Significance: The genetic support of NDM-1 gene suggests that it has evolved through complex pathways. The association with broad host range plasmid and multiple mobile genetic elements explain its observed horizontal mobility in multiple bacterial taxa. © 2011 Ho et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacterial Proteins - genetics-
dc.subject.meshEscherichia coli - enzymology-
dc.subject.meshHong Kong-
dc.subject.meshPlasmids-
dc.subject.meshbeta-Lactamases - genetics-
dc.titleComplete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH: khchowb@hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0017989en_HK
dc.identifier.pmid21445317-
dc.identifier.pmcidPMC3061923-
dc.identifier.scopuseid_2-s2.0-79952838766en_HK
dc.identifier.hkuros191834en_US
dc.identifier.hkuros219372-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952838766&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue3en_HK
dc.identifier.spagee17989en_US
dc.identifier.epagee17989en_US
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000288613300025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLo, WU=35558916700en_HK
dc.identifier.scopusauthoridYeung, MK=37040000300en_HK
dc.identifier.scopusauthoridLin, CH=44761160100en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridAng, I=55214129400en_HK
dc.identifier.scopusauthoridTong, AHY=7103351716en_HK
dc.identifier.scopusauthoridBaoJessie, J YJ=37041318000en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridLo, JYC=7201650939en_HK
dc.customcontrol.immutablejt 130814-

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