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Article: Intracerebroventricular injection of epidermal growth factor reduces neurological deficit and infarct volume and enhances nestin expression following focal cerebral infarction in adult hypertensive rats

TitleIntracerebroventricular injection of epidermal growth factor reduces neurological deficit and infarct volume and enhances nestin expression following focal cerebral infarction in adult hypertensive rats
Authors
KeywordsGlial fibrillary acidic protein
Middle cerebral artery occlusion
Neural plasticity
Renovascular hypertension
Issue Date2009
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEP
Citation
Clinical And Experimental Pharmacology And Physiology, 2009, v. 36 n. 5-6, p. 539-546 How to Cite?
AbstractStudies have documented the proliferative effects of epidermal growth factor (EGF) on neural progenitor cells in the normal or injured brain. The effect of EGF on post-stroke cerebral expression of nestin, a marker of neural progenitor cells, has not been examined in hypertensive rats. In the present study, adult renovascular hypertensive Sprague-Dawley rats underwent either real or sham middle cerebral artery occlusion (MCAO). Intracerebroventricular injections of either 1 g EGF or vehicle (0.01 mol/L phosphate-buffered saline containing 0.1 mg/mL rat serum albumin) were made 24 and 48 h after MCAO. Then, 1, 2, 3 and 4 weeks after MCAO, the postural reflex was evaluated in a blinded fashion before rat brains were processed to determine the infarct volume plus immunoreactivity for nestin and/or glial fibrillary acidic protein (GFAP). Another group of rats was used to quantify nestin expression using western blot analysis. Middle cerebral artery occlusion resulted in a focal infarct that was largest at 1 week and diminished gradually over the time. The impaired postural reflex followed a similar time-course. In addition, MCAO induced a marked increase in nestin expression in both hemispheres, with a higher expression in the right hemisphere; this change was maximal at 1 week and largely subsided at 3 or 4 weeks. Within the right hemisphere, nestin expression was most pronounced in the subventricular and peri-infarct zones. Most nestin-immunoreactive cells were also positive for GFAP. Thus, EGF treatment significantly increases nestin expression, reduces infarct volume and ameliorates postural reflex impairment in adult hypertensive rats. © 2008 Blackwell Publishing Asia Pty Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/137400
ISSN
2012 Impact Factor: 2.16
2015 SCImago Journal Rankings: 0.944
ISI Accession Number ID
Funding AgencyGrant Number
Clinical Priority Subject for Chinese Ministry of Health (2004)
Chinese Medical Board of the USACMB00-730
Natural Science Foundation of Guangdong Province, China2003B30303
2003D30301
Funding Information:

This work was supported by grants from the Clinical Priority Subject for Chinese Ministry of Health (2004), Chinese Medical Board of the USA (CMB00-730) and the Key and Scientific Project of the Natural Science Foundation of Guangdong Province, China (2003B30303 and 2003D30301).

References

 

DC FieldValueLanguage
dc.contributor.authorYu, Jen_HK
dc.contributor.authorZeng, Jen_HK
dc.contributor.authorCheung, RTen_HK
dc.contributor.authorXiong, Len_HK
dc.contributor.authorHe, Men_HK
dc.contributor.authorLiang, Zen_HK
dc.contributor.authorHong, Hen_HK
dc.contributor.authorHuang, Ren_HK
dc.date.accessioned2011-08-26T14:24:21Z-
dc.date.available2011-08-26T14:24:21Z-
dc.date.issued2009en_HK
dc.identifier.citationClinical And Experimental Pharmacology And Physiology, 2009, v. 36 n. 5-6, p. 539-546en_HK
dc.identifier.issn0305-1870en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137400-
dc.description.abstractStudies have documented the proliferative effects of epidermal growth factor (EGF) on neural progenitor cells in the normal or injured brain. The effect of EGF on post-stroke cerebral expression of nestin, a marker of neural progenitor cells, has not been examined in hypertensive rats. In the present study, adult renovascular hypertensive Sprague-Dawley rats underwent either real or sham middle cerebral artery occlusion (MCAO). Intracerebroventricular injections of either 1 g EGF or vehicle (0.01 mol/L phosphate-buffered saline containing 0.1 mg/mL rat serum albumin) were made 24 and 48 h after MCAO. Then, 1, 2, 3 and 4 weeks after MCAO, the postural reflex was evaluated in a blinded fashion before rat brains were processed to determine the infarct volume plus immunoreactivity for nestin and/or glial fibrillary acidic protein (GFAP). Another group of rats was used to quantify nestin expression using western blot analysis. Middle cerebral artery occlusion resulted in a focal infarct that was largest at 1 week and diminished gradually over the time. The impaired postural reflex followed a similar time-course. In addition, MCAO induced a marked increase in nestin expression in both hemispheres, with a higher expression in the right hemisphere; this change was maximal at 1 week and largely subsided at 3 or 4 weeks. Within the right hemisphere, nestin expression was most pronounced in the subventricular and peri-infarct zones. Most nestin-immunoreactive cells were also positive for GFAP. Thus, EGF treatment significantly increases nestin expression, reduces infarct volume and ameliorates postural reflex impairment in adult hypertensive rats. © 2008 Blackwell Publishing Asia Pty Ltd.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEPen_HK
dc.relation.ispartofClinical and Experimental Pharmacology and Physiologyen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectGlial fibrillary acidic proteinen_HK
dc.subjectMiddle cerebral artery occlusionen_HK
dc.subjectNeural plasticityen_HK
dc.subjectRenovascular hypertensionen_HK
dc.subject.meshCerebral Infarction - complications - drug therapy - pathology - physiopathology-
dc.subject.meshEpidermal Growth Factor - administration and dosage - pharmacology-
dc.subject.meshHypertension - complications - drug therapy - metabolism - pathology-
dc.subject.meshIntermediate Filament Proteins - metabolism-
dc.subject.meshMental Disorders - pathology - physiopathology - prevention and control-
dc.titleIntracerebroventricular injection of epidermal growth factor reduces neurological deficit and infarct volume and enhances nestin expression following focal cerebral infarction in adult hypertensive ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1870&volume=36&issue=5-6&spage=539&epage=546&date=2009&atitle=Intracerebroventricular+injection+of+epidermal+growth+factor+reduces+neurological+deficit+and+infarct+volume+and+enhances+nestin+expression+following+focal+cerebral+infarction+in+adult+hypertensive+rats-
dc.identifier.emailCheung, RT:rtcheung@hku.hken_HK
dc.identifier.authorityCheung, RT=rp00434en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1681.2008.05105.xen_HK
dc.identifier.pmid19673937-
dc.identifier.scopuseid_2-s2.0-65349095500en_HK
dc.identifier.hkuros190123en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65349095500&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage539en_HK
dc.identifier.epage546en_HK
dc.identifier.isiWOS:000265549200013-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridYu, J=7405525088en_HK
dc.identifier.scopusauthoridZeng, J=7402652310en_HK
dc.identifier.scopusauthoridCheung, RT=7202397498en_HK
dc.identifier.scopusauthoridXiong, L=55217049900en_HK
dc.identifier.scopusauthoridHe, M=7402609337en_HK
dc.identifier.scopusauthoridLiang, Z=16425733700en_HK
dc.identifier.scopusauthoridHong, H=7401521368en_HK
dc.identifier.scopusauthoridHuang, R=7402950301en_HK
dc.identifier.citeulike4447161-

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