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Article: The hepatitis B virus-associated estrogen receptor alpha (ERα) was regulated by microRNA-130a in HepG2.2.15 human hepatocellular carcinoma cells

TitleThe hepatitis B virus-associated estrogen receptor alpha (ERα) was regulated by microRNA-130a in HepG2.2.15 human hepatocellular carcinoma cells
Authors
Keywordsestrogen receptor α
hepatitis B virus
microRNA
miR-130a
Issue Date2011
PublisherOxford University Press. The Journal's website is located at http://abbs.oxfordjournals.org/
Citation
Acta Biochimica Et Biophysica Sinica, 2011, v. 43 n. 8, p. 640-646 How to Cite?
AbstractThe estrogen receptors (ERs) play important roles in hepatocarcinogenesis, which is always fostered by persistent hepatitis B virus (HBV) infection. Recent studies have linked microRNAs (miRNAs) to viral pathogenesis or oncogenesis. ERα could lead to cell cycle progression or inhibition of apoptosis, while ERβ had opposite effects. Here we proposed that HBV affected ERs expression in viral oncogenesis, which might be triggered by miRNAs. The protein expression of ERα in HepG2.2.15 cells was much stronger than that in HepG2 cells, which was not consistent with its mRNA expression in these cells. MicroRNA-130a (miR-130a) was predicted to be a regulator of ERα by targeting its 3′ untranslated region (3′-UTR). The enhanced green fluorescence protein (EGFP) reporter experiment confirmed the direct interaction of miR-130a and ERα. Moreover, ERα protein level was inversely correlated with the miR-130a level. Taken together, our studies supported that HBV infection might attenuate miR-130a expression and ERα was a direct target of miR-130a. Difference in miR-130a levels between HepG2 and HepG2.2.15 cells resulted in the difference in ERα expression, implying host-virus crosstalk in viral pathogenesis mediated by miRNAs. © The Author 2011.
Persistent Identifierhttp://hdl.handle.net/10722/137380
ISSN
2015 Impact Factor: 2.124
2015 SCImago Journal Rankings: 0.881
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, Len_HK
dc.contributor.authorPu, Yen_HK
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorLiu, Ten_HK
dc.contributor.authorTang, Hen_HK
dc.contributor.authorXiang, Ten_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorRen, Gen_HK
dc.date.accessioned2011-08-26T14:24:09Z-
dc.date.available2011-08-26T14:24:09Z-
dc.date.issued2011en_HK
dc.identifier.citationActa Biochimica Et Biophysica Sinica, 2011, v. 43 n. 8, p. 640-646en_HK
dc.identifier.issn1672-9145en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137380-
dc.description.abstractThe estrogen receptors (ERs) play important roles in hepatocarcinogenesis, which is always fostered by persistent hepatitis B virus (HBV) infection. Recent studies have linked microRNAs (miRNAs) to viral pathogenesis or oncogenesis. ERα could lead to cell cycle progression or inhibition of apoptosis, while ERβ had opposite effects. Here we proposed that HBV affected ERs expression in viral oncogenesis, which might be triggered by miRNAs. The protein expression of ERα in HepG2.2.15 cells was much stronger than that in HepG2 cells, which was not consistent with its mRNA expression in these cells. MicroRNA-130a (miR-130a) was predicted to be a regulator of ERα by targeting its 3′ untranslated region (3′-UTR). The enhanced green fluorescence protein (EGFP) reporter experiment confirmed the direct interaction of miR-130a and ERα. Moreover, ERα protein level was inversely correlated with the miR-130a level. Taken together, our studies supported that HBV infection might attenuate miR-130a expression and ERα was a direct target of miR-130a. Difference in miR-130a levels between HepG2 and HepG2.2.15 cells resulted in the difference in ERα expression, implying host-virus crosstalk in viral pathogenesis mediated by miRNAs. © The Author 2011.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's website is located at http://abbs.oxfordjournals.org/-
dc.relation.ispartofActa Biochimica et Biophysica Sinicaen_HK
dc.subjectestrogen receptor αen_HK
dc.subjecthepatitis B virusen_HK
dc.subjectmicroRNAen_HK
dc.subjectmiR-130aen_HK
dc.titleThe hepatitis B virus-associated estrogen receptor alpha (ERα) was regulated by microRNA-130a in HepG2.2.15 human hepatocellular carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1672-9145&volume=43&issue=8&spage=640&epage=646&date=2011&atitle=The+hepatitis+B+virus-associated+estrogen+receptor+alpha+(ERα)+was+regulated+by+microRNA-130a+in+HepG2.2.15+human+hepatocellular+carcinoma+cells-
dc.identifier.emailWong, DKH:danywong@hku.hken_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/abbs/gmr051en_HK
dc.identifier.pmid21712254-
dc.identifier.scopuseid_2-s2.0-79961082599en_HK
dc.identifier.hkuros189837en_US
dc.identifier.hkuros211255-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79961082599&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue8en_HK
dc.identifier.spage640en_HK
dc.identifier.epage646en_HK
dc.identifier.isiWOS:000293299600008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTang, L=45561695500en_HK
dc.identifier.scopusauthoridPu, Y=35189384300en_HK
dc.identifier.scopusauthoridWong, DKH=7401535819en_HK
dc.identifier.scopusauthoridLiu, T=37102150800en_HK
dc.identifier.scopusauthoridTang, H=7403125088en_HK
dc.identifier.scopusauthoridXiang, T=7006837617en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridRen, G=25422664000en_HK

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