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Article: In Vivo Proton Magnetic Resonance Spectroscopy of Hepatic Ischemia/Reperfusion Injury in an Experimental Model

TitleIn Vivo Proton Magnetic Resonance Spectroscopy of Hepatic Ischemia/Reperfusion Injury in an Experimental Model
Authors
KeywordsCholine
Ischemia/reperfusion injury
Liver
MRI
Spectroscopy
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/arad
Citation
Academic Radiology, 2011, v. 18 n. 2, p. 246-252 How to Cite?
AbstractRationale and Objectives: Hepatic ischemia/reperfusion injury (IRI) occurs during certain hepatobiliary surgeries, hemorrhagic shock, and veno-occlusive disease. Biochemical changes caused by hepatic IRI lead to hepatocellular remodeling, including cellular regeneration or irreversible apoptosis. This study aims to characterize and monitor the metabolic changes in hepatic IRI using proton magnetic resonance spectroscopy (1H MRS). Materials and Methods: Sprague-Dawley rats (n = 8) were scanned with 1H MRS using 5.0 × 5.0 × 5.0 mm3 voxel over a homogeneous liver parenchyma at 7 Tesla with a respiratory-gated point-resolved spectroscopy sequence at 1 day before, 6 hours, 1 day, and 1 week after 30 minutes total hepatic IRI. Signal integral ratios of choline-containing compounds (CCC), glycogen and glucose complex (Glyu), methylene proton ((-CH2-)n), and methene proton (-CH=CH-) to lipid (integral sum of methyl proton (-CH3), (-CH2-)n and -CH=CH-) were quantified by areas under peaks longitudinally. Results: The CCC-to-lipid and Glyu-to-lipid ratios at 6 hours after IRI were significantly higher than those at 1 day before, 1 day, and 1 week after injury. The (-CH2-)n-to-lipid, and -CH=CH-to-lipid ratios showed no significant differences over different time points. Hepatocellular regeneration was observed at 6 hours after IRI in histology with immunohistochemical technique. Conclusions: Changes in CCC-to-lipid and Glyu-to-lipid ratios likely reflect the hepatocellular remodeling and impaired glucose utilization upon hepatic IRI, respectively. The experimental findings in the current study demonstrated that 1H MRS is a valuable tool for characterizing either global or regional metabolic changes in liver noninvasively and longitudinally. Such capability has the potential to lead to early diagnosis and detection of impaired liver function. © 2011 AUR.
Persistent Identifierhttp://hdl.handle.net/10722/137282
ISSN
2015 Impact Factor: 1.966
2015 SCImago Journal Rankings: 1.008
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilGRF HKU7793/08M
Funding Information:

From the Laboratory of Biomedical Imaging and Signal Processing (A.M.C., K.W.Y.C., S.J.F., J.Y., J.S.C., E.X.W.) and Departments of Electrical and Electronic Engineering (A.M.C., K.W.Y.C., S.J.F., J.Y., J.S.C., E.X.W.), Diagnostic Radiology (P.-L.K.), and Medicine (E.X.W.), The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Department of Diagnostic Radiology of the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an, Shannxi Province, China (J.Y.). Received April 8, 2010; accepted September 20, 2010. Supported in part by Hong Kong Research Grant Council (GRF HKU7793/08M). Address correspondence to: E.X.W. e-mail: ewu@eee.hku.hk

References

 

DC FieldValueLanguage
dc.contributor.authorChow, AMen_HK
dc.contributor.authorChan, KWYen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorCheung, JSen_HK
dc.contributor.authorKhong, PLen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2011-08-26T14:22:30Z-
dc.date.available2011-08-26T14:22:30Z-
dc.date.issued2011en_HK
dc.identifier.citationAcademic Radiology, 2011, v. 18 n. 2, p. 246-252en_HK
dc.identifier.issn1076-6332en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137282-
dc.description.abstractRationale and Objectives: Hepatic ischemia/reperfusion injury (IRI) occurs during certain hepatobiliary surgeries, hemorrhagic shock, and veno-occlusive disease. Biochemical changes caused by hepatic IRI lead to hepatocellular remodeling, including cellular regeneration or irreversible apoptosis. This study aims to characterize and monitor the metabolic changes in hepatic IRI using proton magnetic resonance spectroscopy (1H MRS). Materials and Methods: Sprague-Dawley rats (n = 8) were scanned with 1H MRS using 5.0 × 5.0 × 5.0 mm3 voxel over a homogeneous liver parenchyma at 7 Tesla with a respiratory-gated point-resolved spectroscopy sequence at 1 day before, 6 hours, 1 day, and 1 week after 30 minutes total hepatic IRI. Signal integral ratios of choline-containing compounds (CCC), glycogen and glucose complex (Glyu), methylene proton ((-CH2-)n), and methene proton (-CH=CH-) to lipid (integral sum of methyl proton (-CH3), (-CH2-)n and -CH=CH-) were quantified by areas under peaks longitudinally. Results: The CCC-to-lipid and Glyu-to-lipid ratios at 6 hours after IRI were significantly higher than those at 1 day before, 1 day, and 1 week after injury. The (-CH2-)n-to-lipid, and -CH=CH-to-lipid ratios showed no significant differences over different time points. Hepatocellular regeneration was observed at 6 hours after IRI in histology with immunohistochemical technique. Conclusions: Changes in CCC-to-lipid and Glyu-to-lipid ratios likely reflect the hepatocellular remodeling and impaired glucose utilization upon hepatic IRI, respectively. The experimental findings in the current study demonstrated that 1H MRS is a valuable tool for characterizing either global or regional metabolic changes in liver noninvasively and longitudinally. Such capability has the potential to lead to early diagnosis and detection of impaired liver function. © 2011 AUR.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/araden_HK
dc.relation.ispartofAcademic Radiologyen_HK
dc.subjectCholineen_HK
dc.subjectIschemia/reperfusion injuryen_HK
dc.subjectLiveren_HK
dc.subjectMRIen_HK
dc.subjectSpectroscopyen_HK
dc.subject.meshCholine - metabolism-
dc.subject.meshLipid Metabolism-
dc.subject.meshLiver - blood supply - metabolism-
dc.subject.meshMagnetic Resonance Spectroscopy - diagnostic use-
dc.subject.meshReperfusion Injury - diagnosis - metabolism-
dc.titleIn Vivo Proton Magnetic Resonance Spectroscopy of Hepatic Ischemia/Reperfusion Injury in an Experimental Modelen_HK
dc.typeArticleen_HK
dc.identifier.emailKhong, PL:plkhong@hkucc.hku.hken_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authorityKhong, PL=rp00467en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.acra.2010.09.019en_HK
dc.identifier.pmid21111640-
dc.identifier.scopuseid_2-s2.0-78651108437en_HK
dc.identifier.hkuros191958en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78651108437&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue2en_HK
dc.identifier.spage246en_HK
dc.identifier.epage252en_HK
dc.identifier.isiWOS:000286699200017-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridChan, KWY=35763564900en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridYang, J=23391308100en_HK
dc.identifier.scopusauthoridCheung, JS=16174280400en_HK
dc.identifier.scopusauthoridKhong, PL=7006693233en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.citeulike8345771-

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