Article: Interleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression
| Title | Interleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Li, J1 Lau, GKK1 Chen, L1 Dong, Ss1 Lan, HY4 Huang, XR4 Li, Y1 Luk, JM2 Yuan, YF3 Guan, Xy1 3 | ||||||||
| Issue Date | 2011 | ||||||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||||
| Citation | Plos One, 2011, v. 6 n. 7 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0021816 | ||||||||
| Abstract | Background: IL-17A is a pro-inflammatory cytokine that plays important role in inflammatory disease pathology and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the progression of hepatocellular carcinoma (HCC). Methodology and Principal Finding: Expression pattern of IL-17A in clinical HCC samples (n = 43) was determined by immunohistochemistry staining. Transcript levels of MMP2, MMP9 and IL-17A were measured in another 50 pairs (including tumor and related non-tumor tissues) HCC samples. Cell growth, focus formation, cell migration, invasion and western blot assays were used to characterize the functional and signaling mechanisms in IL-17A-treated HCC. Association study was used to identify clinical significance of IL-17A in HCC. Compared with paired non-tumor tissue, higher frequency of IL-17A-positive cells was detected in tumor tissues in HCCs with metastasis, and the frequency of IL-17A-positive cells was also significantly associated with poor prognosis of HCC (P = 0.01). Functional study found that IL-17A could promote HCC cell migration and invasion. Further molecular analysis also showed that IL-17A could upregulate MMP2 and MMP9 expression via NF-κB signaling activation. Conclusions: IL-17A could promote HCC metastasis by the upregulation of MMP2 and MMP9 expression via activating NF-κB signaling pathway. © 2011 Li et al. | ||||||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0021816 | ||||||||
| ISI Accession Number ID | WOS:000292655400021
Funding Information: This work was supported by a Hong Kong Research Grant Council Grant (HKU 7656/07M), Hong Kong RGC Collaborative Research Grants (HKU5/CRF/08 and HKU7/CRG09) and the "Hundred Talents Program'' at Sun Yat-sen University (85000-3171311). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||||
| PubMed Central ID | PMC3131399 | ||||||||
| References | References in Scopus | ||||||||
| Grants | Liver Transplantation Research Centre: A Multidisciplinary Study for Liver Graft Injury |
| dc.contributor.author | Li, J | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Lau, GKK | ||||||||
| dc.contributor.author | Chen, L | ||||||||
| dc.contributor.author | Dong, Ss | ||||||||
| dc.contributor.author | Lan, HY | ||||||||
| dc.contributor.author | Huang, XR | ||||||||
| dc.contributor.author | Li, Y | ||||||||
| dc.contributor.author | Luk, JM | ||||||||
| dc.contributor.author | Yuan, YF | ||||||||
| dc.contributor.author | Guan, Xy | ||||||||
| dc.date.accessioned | 2011-08-26T14:22:19Z | ||||||||
| dc.date.available | 2011-08-26T14:22:19Z | ||||||||
| dc.date.issued | 2011 | ||||||||
| dc.description.abstract | Background: IL-17A is a pro-inflammatory cytokine that plays important role in inflammatory disease pathology and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the progression of hepatocellular carcinoma (HCC). Methodology and Principal Finding: Expression pattern of IL-17A in clinical HCC samples (n = 43) was determined by immunohistochemistry staining. Transcript levels of MMP2, MMP9 and IL-17A were measured in another 50 pairs (including tumor and related non-tumor tissues) HCC samples. Cell growth, focus formation, cell migration, invasion and western blot assays were used to characterize the functional and signaling mechanisms in IL-17A-treated HCC. Association study was used to identify clinical significance of IL-17A in HCC. Compared with paired non-tumor tissue, higher frequency of IL-17A-positive cells was detected in tumor tissues in HCCs with metastasis, and the frequency of IL-17A-positive cells was also significantly associated with poor prognosis of HCC (P = 0.01). Functional study found that IL-17A could promote HCC cell migration and invasion. Further molecular analysis also showed that IL-17A could upregulate MMP2 and MMP9 expression via NF-κB signaling activation. Conclusions: IL-17A could promote HCC metastasis by the upregulation of MMP2 and MMP9 expression via activating NF-κB signaling pathway. © 2011 Li et al. | ||||||||
| dc.description.grant | Liver Transplantation Research Centre: A Multidisciplinary Study for Liver Graft Injury | ||||||||
| dc.description.grantcode | 99532 | ||||||||
| dc.description.nature | published_or_final_version | ||||||||
| dc.identifier.citation | Plos One, 2011, v. 6 n. 7 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0021816 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0021816 | ||||||||
| dc.identifier.epage | e21816 | ||||||||
| dc.identifier.hkuros | 190890 | ||||||||
| dc.identifier.isi | WOS:000292655400021
Funding Information: This work was supported by a Hong Kong Research Grant Council Grant (HKU 7656/07M), Hong Kong RGC Collaborative Research Grants (HKU5/CRF/08 and HKU7/CRG09) and the "Hundred Talents Program'' at Sun Yat-sen University (85000-3171311). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||||
| dc.identifier.issue | 7 | ||||||||
| dc.identifier.pmcid | PMC3131399 | ||||||||
| dc.identifier.pmid | 21760911 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-79960061468 | ||||||||
| dc.identifier.spage | e21816 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/137273 | ||||||||
| dc.identifier.volume | 6 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||||
| dc.publisher.place | United States | ||||||||
| dc.relation.ispartof | PLoS ONE | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||
| dc.subject.mesh | Carcinoma, Hepatocellular - enzymology - genetics - pathology | ||||||||
| dc.subject.mesh | Interleukin-17 - pharmacology | ||||||||
| dc.subject.mesh | Liver Neoplasms - enzymology - genetics - pathology | ||||||||
| dc.subject.mesh | Matrix Metalloproteinase 2 - biosynthesis - genetics | ||||||||
| dc.subject.mesh | Matrix Metalloproteinase 9 - biosynthesis - genetics | ||||||||
| dc.title | Interleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression | ||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- Yong Loo Lin School of Medicine
- Sun Yat-Sen University Cancer Center
- The University of Hong Kong