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Article: Interleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression
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TitleInterleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression
 
AuthorsLi, J1
Lau, GKK1
Chen, L1
Dong, Ss1
Lan, HY4
Huang, XR4
Li, Y1
Luk, JM2
Yuan, YF3
Guan, Xy1 3
 
Issue Date2011
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPlos One, 2011, v. 6 n. 7 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0021816
 
AbstractBackground: IL-17A is a pro-inflammatory cytokine that plays important role in inflammatory disease pathology and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the progression of hepatocellular carcinoma (HCC). Methodology and Principal Finding: Expression pattern of IL-17A in clinical HCC samples (n = 43) was determined by immunohistochemistry staining. Transcript levels of MMP2, MMP9 and IL-17A were measured in another 50 pairs (including tumor and related non-tumor tissues) HCC samples. Cell growth, focus formation, cell migration, invasion and western blot assays were used to characterize the functional and signaling mechanisms in IL-17A-treated HCC. Association study was used to identify clinical significance of IL-17A in HCC. Compared with paired non-tumor tissue, higher frequency of IL-17A-positive cells was detected in tumor tissues in HCCs with metastasis, and the frequency of IL-17A-positive cells was also significantly associated with poor prognosis of HCC (P = 0.01). Functional study found that IL-17A could promote HCC cell migration and invasion. Further molecular analysis also showed that IL-17A could upregulate MMP2 and MMP9 expression via NF-κB signaling activation. Conclusions: IL-17A could promote HCC metastasis by the upregulation of MMP2 and MMP9 expression via activating NF-κB signaling pathway. © 2011 Li et al.
 
ISSN1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0021816
 
PubMed Central IDPMC3131399
 
ISI Accession Number IDWOS:000292655400021
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 7656/07M
Hong Kong RGCHKU5/CRF/08
HKU7/CRG09
Sun Yat-sen University85000-3171311
Funding Information:

This work was supported by a Hong Kong Research Grant Council Grant (HKU 7656/07M), Hong Kong RGC Collaborative Research Grants (HKU5/CRF/08 and HKU7/CRG09) and the "Hundred Talents Program'' at Sun Yat-sen University (85000-3171311). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
GrantsLiver Transplantation Research Centre: A Multidisciplinary Study for Liver Graft Injury
 
DC FieldValue
dc.contributor.authorLi, J
 
dc.contributor.authorLau, GKK
 
dc.contributor.authorChen, L
 
dc.contributor.authorDong, Ss
 
dc.contributor.authorLan, HY
 
dc.contributor.authorHuang, XR
 
dc.contributor.authorLi, Y
 
dc.contributor.authorLuk, JM
 
dc.contributor.authorYuan, YF
 
dc.contributor.authorGuan, Xy
 
dc.date.accessioned2011-08-26T14:22:19Z
 
dc.date.available2011-08-26T14:22:19Z
 
dc.date.issued2011
 
dc.description.abstractBackground: IL-17A is a pro-inflammatory cytokine that plays important role in inflammatory disease pathology and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the progression of hepatocellular carcinoma (HCC). Methodology and Principal Finding: Expression pattern of IL-17A in clinical HCC samples (n = 43) was determined by immunohistochemistry staining. Transcript levels of MMP2, MMP9 and IL-17A were measured in another 50 pairs (including tumor and related non-tumor tissues) HCC samples. Cell growth, focus formation, cell migration, invasion and western blot assays were used to characterize the functional and signaling mechanisms in IL-17A-treated HCC. Association study was used to identify clinical significance of IL-17A in HCC. Compared with paired non-tumor tissue, higher frequency of IL-17A-positive cells was detected in tumor tissues in HCCs with metastasis, and the frequency of IL-17A-positive cells was also significantly associated with poor prognosis of HCC (P = 0.01). Functional study found that IL-17A could promote HCC cell migration and invasion. Further molecular analysis also showed that IL-17A could upregulate MMP2 and MMP9 expression via NF-κB signaling activation. Conclusions: IL-17A could promote HCC metastasis by the upregulation of MMP2 and MMP9 expression via activating NF-κB signaling pathway. © 2011 Li et al.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPlos One, 2011, v. 6 n. 7 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0021816
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0021816
 
dc.identifier.epagee21816
 
dc.identifier.hkuros190890
 
dc.identifier.isiWOS:000292655400021
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 7656/07M
Hong Kong RGCHKU5/CRF/08
HKU7/CRG09
Sun Yat-sen University85000-3171311
Funding Information:

This work was supported by a Hong Kong Research Grant Council Grant (HKU 7656/07M), Hong Kong RGC Collaborative Research Grants (HKU5/CRF/08 and HKU7/CRG09) and the "Hundred Talents Program'' at Sun Yat-sen University (85000-3171311). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.512
 
dc.identifier.issue7
 
dc.identifier.pmcidPMC3131399
 
dc.identifier.pmid21760911
 
dc.identifier.scopuseid_2-s2.0-79960061468
 
dc.identifier.spagee21816
 
dc.identifier.urihttp://hdl.handle.net/10722/137273
 
dc.identifier.volume6
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS ONE
 
dc.relation.projectLiver Transplantation Research Centre: A Multidisciplinary Study for Liver Graft Injury
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCarcinoma, Hepatocellular - enzymology - genetics - pathology
 
dc.subject.meshInterleukin-17 - pharmacology
 
dc.subject.meshLiver Neoplasms - enzymology - genetics - pathology
 
dc.subject.meshMatrix Metalloproteinase 2 - biosynthesis - genetics
 
dc.subject.meshMatrix Metalloproteinase 9 - biosynthesis - genetics
 
dc.titleInterleukin 17a promotes hepatocellular carcinoma metastasis via NF-kB induced matrix metalloproteinases 2 and 9 expression
 
dc.typeArticle
 
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<contributor.author>Lan, HY</contributor.author>
<contributor.author>Huang, XR</contributor.author>
<contributor.author>Li, Y</contributor.author>
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<description.abstract>Background: IL-17A is a pro-inflammatory cytokine that plays important role in inflammatory disease pathology and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the progression of hepatocellular carcinoma (HCC). Methodology and Principal Finding: Expression pattern of IL-17A in clinical HCC samples (n = 43) was determined by immunohistochemistry staining. Transcript levels of MMP2, MMP9 and IL-17A were measured in another 50 pairs (including tumor and related non-tumor tissues) HCC samples. Cell growth, focus formation, cell migration, invasion and western blot assays were used to characterize the functional and signaling mechanisms in IL-17A-treated HCC. Association study was used to identify clinical significance of IL-17A in HCC. Compared with paired non-tumor tissue, higher frequency of IL-17A-positive cells was detected in tumor tissues in HCCs with metastasis, and the frequency of IL-17A-positive cells was also significantly associated with poor prognosis of HCC (P = 0.01). Functional study found that IL-17A could promote HCC cell migration and invasion. Further molecular analysis also showed that IL-17A could upregulate MMP2 and MMP9 expression via NF-&#954;B signaling activation. Conclusions: IL-17A could promote HCC metastasis by the upregulation of MMP2 and MMP9 expression via activating NF-&#954;B signaling pathway. &#169; 2011 Li et al.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Yong Loo Lin School of Medicine
  3. Sun Yat-Sen University Cancer Center
  4. The University of Hong Kong