File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Overexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinoma

TitleOverexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinoma
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccancer/
Citation
Bmc Cancer, 2011, v. 11 How to Cite?
Abstract
Background: By using cDNA microarray analysis, we identified a G protein-coupled receptor, GPR39, that is significantly up-regulated in ESCC. The aim of this study is to investigate the role of GPR39 in human esophageal cancer development, and to examine the prevalence and clinical significance of GPR39 overexpression in ESCC.Methods: The mRNA expression level of GPR39 was analyzed in 9 ESCC cell lines and 50 primary ESCC tumors using semi-quantitative RT-PCR. Immunohistochemistry was used to assess GPR39 protein expression in tissue arrays containing 300 primary ESCC cases. In vitro and in vivo studies were done to elucidate the tumorigenic role of GPR39 in ESCC cells.Results: We found that GPR39 was frequently overexpressed in primary ESCCs in both mRNA level (27/50, 54%) and protein level (121/207, 58.5%), which was significantly associated with the lymph node metastasis and advanced TNM stage (P < 0.01). Functional studies showed that GPR39 has a strong tumorigenic ability. Introduction of GPR39 gene into ESCC cell line KYSE30 could promote cell proliferation, increase foci formation, colony formation in soft agar, and tumor formation in nude mice. The mechanism by which amplified GPR39 induces tumorigenesis was associated with its role in promoting G1/S transition via up-regulation of cyclin D1 and CDK6. Further study found GPR39 could enhance cell motility and invasiveness by inducing EMT and remodeling cytoskeleton. Moreover, depletion of endogenous GPR39 by siRNA could effectively decrease the oncogenicity of ESCC cells.Conclusions: The present study suggests that GPR39 plays an important tumorigenic role in the development and progression of ESCC. © 2011 Xie et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/137267
ISSN
2013 Impact Factor: 3.319
2013 SCImago Journal Rankings: 1.686
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Major State Basic Research Program of China2006CB910104
Research Fund for the Doctoral Program of Higher Education of China20070558272
Research Grant Council Central AllocationHKUST 2/06C
Funding Information:

This work was supported by Grants from National Natural Science Foundation of China (30772475, 30700820 and 30971606), Sun Yat-Sen University "Hundred Talents Program" (85000-3171311), Grant from the Major State Basic Research Program of China (2006CB910104), Research Fund for the Doctoral Program of Higher Education of China (20070558272) and Research Grant Council Central Allocation (HKUST 2/06C).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorXie, Fen_HK
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorZhu, YHen_HK
dc.contributor.authorQin, YRen_HK
dc.contributor.authorDai, Yen_HK
dc.contributor.authorZeng, Ten_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorNie, Cen_HK
dc.contributor.authorTang, Hen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorFu, Len_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2011-08-26T14:22:07Z-
dc.date.available2011-08-26T14:22:07Z-
dc.date.issued2011en_HK
dc.identifier.citationBmc Cancer, 2011, v. 11en_HK
dc.identifier.issn1471-2407en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137267-
dc.description.abstractBackground: By using cDNA microarray analysis, we identified a G protein-coupled receptor, GPR39, that is significantly up-regulated in ESCC. The aim of this study is to investigate the role of GPR39 in human esophageal cancer development, and to examine the prevalence and clinical significance of GPR39 overexpression in ESCC.Methods: The mRNA expression level of GPR39 was analyzed in 9 ESCC cell lines and 50 primary ESCC tumors using semi-quantitative RT-PCR. Immunohistochemistry was used to assess GPR39 protein expression in tissue arrays containing 300 primary ESCC cases. In vitro and in vivo studies were done to elucidate the tumorigenic role of GPR39 in ESCC cells.Results: We found that GPR39 was frequently overexpressed in primary ESCCs in both mRNA level (27/50, 54%) and protein level (121/207, 58.5%), which was significantly associated with the lymph node metastasis and advanced TNM stage (P < 0.01). Functional studies showed that GPR39 has a strong tumorigenic ability. Introduction of GPR39 gene into ESCC cell line KYSE30 could promote cell proliferation, increase foci formation, colony formation in soft agar, and tumor formation in nude mice. The mechanism by which amplified GPR39 induces tumorigenesis was associated with its role in promoting G1/S transition via up-regulation of cyclin D1 and CDK6. Further study found GPR39 could enhance cell motility and invasiveness by inducing EMT and remodeling cytoskeleton. Moreover, depletion of endogenous GPR39 by siRNA could effectively decrease the oncogenicity of ESCC cells.Conclusions: The present study suggests that GPR39 plays an important tumorigenic role in the development and progression of ESCC. © 2011 Xie et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccancer/en_HK
dc.relation.ispartofBMC Canceren_HK
dc.rightsBMC Cancer. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCarcinoma, Squamous Cell - genetics - metabolism - pathology-
dc.subject.meshCell Transformation, Neoplastic - genetics - metabolism - pathology-
dc.subject.meshEsophageal Neoplasms - genetics - metabolism - pathology-
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolism - physiology-
dc.subject.meshTransplantation, Heterologous-
dc.titleOverexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailFu, L:gracefu@graduate.hku.hken_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityFu, L=rp01435en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-2407-11-86en_HK
dc.identifier.pmid21352519-
dc.identifier.pmcidPMC3053269-
dc.identifier.scopuseid_2-s2.0-79951958514en_HK
dc.identifier.hkuros190587en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79951958514&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.isiWOS:000288181800001-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectEsophageal Carcinoma Research Center-
dc.relation.projectEsophageal Carcinoma Research Center-
dc.relation.projectEsophageal Carcinoma Research Center-
dc.relation.projectEsophageal Carcinoma Research Center-
dc.relation.projectEsophageal Carcinoma Research Center-
dc.relation.projectEsophageal Carcinoma Research Center-
dc.identifier.scopusauthoridXie, F=22959384700en_HK
dc.identifier.scopusauthoridLiu, H=34877073600en_HK
dc.identifier.scopusauthoridZhu, YH=19338197800en_HK
dc.identifier.scopusauthoridQin, YR=7403100680en_HK
dc.identifier.scopusauthoridDai, Y=37014328400en_HK
dc.identifier.scopusauthoridZeng, T=37015099200en_HK
dc.identifier.scopusauthoridChen, L=23569135400en_HK
dc.identifier.scopusauthoridNie, C=37014834800en_HK
dc.identifier.scopusauthoridTang, H=36946431400en_HK
dc.identifier.scopusauthoridLi, Y=36078298200en_HK
dc.identifier.scopusauthoridFu, L=22979236700en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.citeulike8895393-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats