Article: Overexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinoma

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TitleOverexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinoma
AuthorsXie, F3
Liu, H3
Zhu, YH3
Qin, YR4
Dai, Y3
Zeng, T3
Chen, L1
Nie, C3
Tang, H2
Li, Y3
Fu, L1 3
Guan, XY1 3
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccancer/
CitationBmc Cancer, 2011, v. 11 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1471-2407-11-86
AbstractBackground: By using cDNA microarray analysis, we identified a G protein-coupled receptor, GPR39, that is significantly up-regulated in ESCC. The aim of this study is to investigate the role of GPR39 in human esophageal cancer development, and to examine the prevalence and clinical significance of GPR39 overexpression in ESCC.Methods: The mRNA expression level of GPR39 was analyzed in 9 ESCC cell lines and 50 primary ESCC tumors using semi-quantitative RT-PCR. Immunohistochemistry was used to assess GPR39 protein expression in tissue arrays containing 300 primary ESCC cases. In vitro and in vivo studies were done to elucidate the tumorigenic role of GPR39 in ESCC cells.Results: We found that GPR39 was frequently overexpressed in primary ESCCs in both mRNA level (27/50, 54%) and protein level (121/207, 58.5%), which was significantly associated with the lymph node metastasis and advanced TNM stage (P < 0.01). Functional studies showed that GPR39 has a strong tumorigenic ability. Introduction of GPR39 gene into ESCC cell line KYSE30 could promote cell proliferation, increase foci formation, colony formation in soft agar, and tumor formation in nude mice. The mechanism by which amplified GPR39 induces tumorigenesis was associated with its role in promoting G1/S transition via up-regulation of cyclin D1 and CDK6. Further study found GPR39 could enhance cell motility and invasiveness by inducing EMT and remodeling cytoskeleton. Moreover, depletion of endogenous GPR39 by siRNA could effectively decrease the oncogenicity of ESCC cells.Conclusions: The present study suggests that GPR39 plays an important tumorigenic role in the development and progression of ESCC. © 2011 Xie et al; licensee BioMed Central Ltd.
ISSN1471-2407
2011 Impact Factor: 3.011
2011 SCImago Journal Rankings: 0.342
DOIhttp://dx.doi.org/10.1186/1471-2407-11-86
ISI Accession Number IDWOS:000288181800001
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Major State Basic Research Program of China2006CB910104
Research Fund for the Doctoral Program of Higher Education of China20070558272
Research Grant Council Central AllocationHKUST 2/06C
Funding Information:

This work was supported by Grants from National Natural Science Foundation of China (30772475, 30700820 and 30971606), Sun Yat-Sen University "Hundred Talents Program" (85000-3171311), Grant from the Major State Basic Research Program of China (2006CB910104), Research Fund for the Doctoral Program of Higher Education of China (20070558272) and Research Grant Council Central Allocation (HKUST 2/06C).

PubMed Central IDPMC3053269
ReferencesReferences in Scopus
GrantsEsophageal Carcinoma Research Center
Esophageal Carcinoma Research Center
Esophageal Carcinoma Research Center
Esophageal Carcinoma Research Center
Esophageal Carcinoma Research Center
Esophageal Carcinoma Research Center
DC Field
Value
dc.contributor.authorXie, F
dc.contributor.authorLiu, H
dc.contributor.authorZhu, YH
dc.contributor.authorQin, YR
dc.contributor.authorDai, Y
dc.contributor.authorZeng, T
dc.contributor.authorChen, L
dc.contributor.authorNie, C
dc.contributor.authorTang, H
dc.contributor.authorLi, Y
dc.contributor.authorFu, L
dc.contributor.authorGuan, XY
dc.date.accessioned2011-08-26T14:22:07Z
dc.date.available2011-08-26T14:22:07Z
dc.date.issued2011
dc.description.abstractBackground: By using cDNA microarray analysis, we identified a G protein-coupled receptor, GPR39, that is significantly up-regulated in ESCC. The aim of this study is to investigate the role of GPR39 in human esophageal cancer development, and to examine the prevalence and clinical significance of GPR39 overexpression in ESCC.Methods: The mRNA expression level of GPR39 was analyzed in 9 ESCC cell lines and 50 primary ESCC tumors using semi-quantitative RT-PCR. Immunohistochemistry was used to assess GPR39 protein expression in tissue arrays containing 300 primary ESCC cases. In vitro and in vivo studies were done to elucidate the tumorigenic role of GPR39 in ESCC cells.Results: We found that GPR39 was frequently overexpressed in primary ESCCs in both mRNA level (27/50, 54%) and protein level (121/207, 58.5%), which was significantly associated with the lymph node metastasis and advanced TNM stage (P < 0.01). Functional studies showed that GPR39 has a strong tumorigenic ability. Introduction of GPR39 gene into ESCC cell line KYSE30 could promote cell proliferation, increase foci formation, colony formation in soft agar, and tumor formation in nude mice. The mechanism by which amplified GPR39 induces tumorigenesis was associated with its role in promoting G1/S transition via up-regulation of cyclin D1 and CDK6. Further study found GPR39 could enhance cell motility and invasiveness by inducing EMT and remodeling cytoskeleton. Moreover, depletion of endogenous GPR39 by siRNA could effectively decrease the oncogenicity of ESCC cells.Conclusions: The present study suggests that GPR39 plays an important tumorigenic role in the development and progression of ESCC. © 2011 Xie et al; licensee BioMed Central Ltd.
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantEsophageal Carcinoma Research Center
dc.description.grantcode96293
dc.description.grantcode96288
dc.description.grantcode96292
dc.description.grantcode96289
dc.description.grantcode96290
dc.description.grantcode96291
dc.description.naturepublished_or_final_version
dc.identifier.citationBmc Cancer, 2011, v. 11 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1471-2407-11-86
dc.identifier.citeulike8895393
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2407-11-86
dc.identifier.hkuros190587
dc.identifier.isiWOS:000288181800001
Funding AgencyGrant Number
National Natural Science Foundation of China30772475
30700820
30971606
Sun Yat-Sen University85000-3171311
Major State Basic Research Program of China2006CB910104
Research Fund for the Doctoral Program of Higher Education of China20070558272
Research Grant Council Central AllocationHKUST 2/06C
Funding Information:

This work was supported by Grants from National Natural Science Foundation of China (30772475, 30700820 and 30971606), Sun Yat-Sen University "Hundred Talents Program" (85000-3171311), Grant from the Major State Basic Research Program of China (2006CB910104), Research Fund for the Doctoral Program of Higher Education of China (20070558272) and Research Grant Council Central Allocation (HKUST 2/06C).

dc.identifier.issn1471-2407
2011 Impact Factor: 3.011
2011 SCImago Journal Rankings: 0.342
dc.identifier.pmcidPMC3053269
dc.identifier.pmid21352519
dc.identifier.scopuseid_2-s2.0-79951958514
dc.identifier.urihttp://hdl.handle.net/10722/137267
dc.identifier.volume11
dc.languageeng
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmccancer/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofBMC Cancer
dc.relation.referencesReferences in Scopus
dc.rightsBMC Cancer. Copyright © BioMed Central Ltd.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshCarcinoma, Squamous Cell - genetics - metabolism - pathology
dc.subject.meshCell Transformation, Neoplastic - genetics - metabolism - pathology
dc.subject.meshEsophageal Neoplasms - genetics - metabolism - pathology
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolism - physiology
dc.subject.meshTransplantation, Heterologous
dc.titleOverexpression of GPR39 contributes to malignant development of human esophageal squamous cell carcinoma
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Zhengzhou University
  3. Sun Yat-Sen University
  4. First Affiliated Hospital of Zhengzhou University