File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Biodegradation of naphthalene by enriched marine denitrifying bacteria

TitleBiodegradation of naphthalene by enriched marine denitrifying bacteria
Authors
KeywordsDenitrifying degradation
Enrichment
NahAc gene
PAHs
QPCR
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration And Biodegradation, 2011, v. 65 n. 1, p. 204-211 How to Cite?
AbstractNumerous studies have been investigated on the PAHs biodegradation in aerobic and anaerobic environments; however, the biodegradation of PAHs under anoxic conditions, especially denitrifying conditions, has drawn less attention. In this study, four series of batch experiments were conducted to investigate the effect of temperature, pH, naphthalene concentration and nitrate concentration on the naphthalene degradation under denitrification condition. Our results showed that the degradation of naphthalene was most favorable at pH 7 and 25°C. Results also indicated that 30mg/l naphthalene inhibited the biodegradation and the removal efficiency was only 20.2%. Significant degradation (91.7% and 96.3%) of naphthalene occurred when nitrate concentrations were 1.0 and 5.0mM. Moreover, the maximum degradation rates were 0.13 and 0.18mg-NAP/(lh) depending on the concentration of nitrate. Based on 16S rDNA analysis, the denitrifying enriched culture was mainly composed of γ-Proteobacteria (19 clones out of a total of 23 clones) and Actinobacteria (4 clones). Using a primer set specific for naphthalene degrading functional gene nahAc, two operational taxonomy units were obtained in the clone library of nahAc. Both of them were closely related to nahAc genes of known species of Pseudomonas. Quantitative polymerase chain reaction (qPCR) was employed to quantify the change of naphthalene-degrading population during the degradation of naphthalene using nahAc gene as the biomarker. The maximum degradation rate and removal efficiency were strongly correlated with nahAc gene copy number, with R 2 of 0.69 and 0.79, respectively. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/137229
ISSN
2015 Impact Factor: 2.429
2015 SCImago Journal Rankings: 0.919
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU7122/08E
Dr. Stephen SF Hui Trust200903173001
HKU
Funding Information:

The authors wish to thank the Hong Kong Research Grants Council (HKU7122/08E) and Dr. Stephen SF Hui Trust Fund (200903173001) for the financial support of this study, and Xiaoying Lu wishes to thank HKU for the postgraduate studentship.

References

 

DC FieldValueLanguage
dc.contributor.authorLu, Xen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorHanPing Fang, Hen_HK
dc.contributor.authorLeung, KMYen_HK
dc.contributor.authorZhang, Gen_HK
dc.date.accessioned2011-08-26T14:21:36Z-
dc.date.available2011-08-26T14:21:36Z-
dc.date.issued2011en_HK
dc.identifier.citationInternational Biodeterioration And Biodegradation, 2011, v. 65 n. 1, p. 204-211en_HK
dc.identifier.issn0964-8305en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137229-
dc.description.abstractNumerous studies have been investigated on the PAHs biodegradation in aerobic and anaerobic environments; however, the biodegradation of PAHs under anoxic conditions, especially denitrifying conditions, has drawn less attention. In this study, four series of batch experiments were conducted to investigate the effect of temperature, pH, naphthalene concentration and nitrate concentration on the naphthalene degradation under denitrification condition. Our results showed that the degradation of naphthalene was most favorable at pH 7 and 25°C. Results also indicated that 30mg/l naphthalene inhibited the biodegradation and the removal efficiency was only 20.2%. Significant degradation (91.7% and 96.3%) of naphthalene occurred when nitrate concentrations were 1.0 and 5.0mM. Moreover, the maximum degradation rates were 0.13 and 0.18mg-NAP/(lh) depending on the concentration of nitrate. Based on 16S rDNA analysis, the denitrifying enriched culture was mainly composed of γ-Proteobacteria (19 clones out of a total of 23 clones) and Actinobacteria (4 clones). Using a primer set specific for naphthalene degrading functional gene nahAc, two operational taxonomy units were obtained in the clone library of nahAc. Both of them were closely related to nahAc genes of known species of Pseudomonas. Quantitative polymerase chain reaction (qPCR) was employed to quantify the change of naphthalene-degrading population during the degradation of naphthalene using nahAc gene as the biomarker. The maximum degradation rate and removal efficiency were strongly correlated with nahAc gene copy number, with R 2 of 0.69 and 0.79, respectively. © 2010 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_HK
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_HK
dc.subjectDenitrifying degradationen_HK
dc.subjectEnrichmenten_HK
dc.subjectNahAc geneen_HK
dc.subjectPAHsen_HK
dc.subjectQPCRen_HK
dc.titleBiodegradation of naphthalene by enriched marine denitrifying bacteriaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0964-8305&volume=65&issue=1&spage=204&epage=211&date=2011&atitle=Biodegradation+of+naphthalene+by+enriched+marine+denitrifying+bacteria-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_HK
dc.identifier.emailHanPing Fang, H: hrechef@hkucc.hku.hken_HK
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityHanPing Fang, H=rp00115en_HK
dc.identifier.authorityLeung, KMY=rp00733en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ibiod.2010.11.004en_HK
dc.identifier.scopuseid_2-s2.0-78650677397en_HK
dc.identifier.hkuros190353en_US
dc.identifier.hkuros208095-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650677397&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue1en_HK
dc.identifier.spage204en_HK
dc.identifier.epage211en_HK
dc.identifier.isiWOS:000286847000031-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLu, X=39561357500en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridHanPing Fang, H=7402542625en_HK
dc.identifier.scopusauthoridLeung, KMY=7401860738en_HK
dc.identifier.scopusauthoridZhang, G=26039620400en_HK
dc.identifier.citeulike8409985-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats