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Article: Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
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TitleHistidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
 
AuthorsGe, R2
Sun, X3
Wang, D2
Zhou, Q2
Sun, H1
 
KeywordsAmyloid
Gastric epithelial cell
Helicobacter pylori
Hpn
Mitochondria
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
 
CitationBiochimica Et Biophysica Acta - Molecular Cell Research, 2011, v. 1813 n. 8, p. 1422-1427 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbamcr.2011.04.005
 
AbstractHelicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.
 
ISSN0167-4889
2013 Impact Factor: 5.297
2013 SCImago Journal Rankings: 2.999
 
DOIhttp://dx.doi.org/10.1016/j.bbamcr.2011.04.005
 
ISI Accession Number IDWOS:000292945200004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGe, R
 
dc.contributor.authorSun, X
 
dc.contributor.authorWang, D
 
dc.contributor.authorZhou, Q
 
dc.contributor.authorSun, H
 
dc.date.accessioned2011-08-26T14:19:15Z
 
dc.date.available2011-08-26T14:19:15Z
 
dc.date.issued2011
 
dc.description.abstractHelicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2011, v. 1813 n. 8, p. 1422-1427 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbamcr.2011.04.005
 
dc.identifier.citeulike9227500
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbamcr.2011.04.005
 
dc.identifier.epage1427
 
dc.identifier.hkuros189548
 
dc.identifier.hkuros211146
 
dc.identifier.isiWOS:000292945200004
 
dc.identifier.issn0167-4889
2013 Impact Factor: 5.297
2013 SCImago Journal Rankings: 2.999
 
dc.identifier.issue8
 
dc.identifier.pmid21539864
 
dc.identifier.scopuseid_2-s2.0-79959365764
 
dc.identifier.spage1422
 
dc.identifier.urihttp://hdl.handle.net/10722/137221
 
dc.identifier.volume1813
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBacterial Proteins - chemistry - physiology - ultrastructure
 
dc.subject.meshEpithelial Cells - microbiology - pathology
 
dc.subject.meshGastric Mucosa - microbiology - pathology
 
dc.subject.meshHelicobacter pylori - pathogenicity - physiology
 
dc.subject.meshProteins - chemistry - physiology - ultrastructure
 
dc.subjectAmyloid
 
dc.subjectGastric epithelial cell
 
dc.subjectHelicobacter pylori
 
dc.subjectHpn
 
dc.subjectMitochondria
 
dc.titleHistidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University
  3. Jinan University