Article: Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells

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TitleHistidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
AuthorsGe, R2
Sun, X3
Wang, D2
Zhou, Q2
Sun, H1
KeywordsAmyloid
Gastric epithelial cell
Helicobacter pylori
Hpn
Mitochondria
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
CitationBiochimica Et Biophysica Acta - Molecular Cell Research, 2011, v. 1813 n. 8, p. 1422-1427 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbamcr.2011.04.005
AbstractHelicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.
ISSN0167-4889
2011 Impact Factor: 5.538
2011 SCImago Journal Rankings: 0.707
DOIhttp://dx.doi.org/10.1016/j.bbamcr.2011.04.005
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorGe, R
dc.contributor.authorSun, X
dc.contributor.authorWang, D
dc.contributor.authorZhou, Q
dc.contributor.authorSun, H
dc.date.accessioned2011-08-26T14:19:15Z
dc.date.available2011-08-26T14:19:15Z
dc.date.issued2011
dc.description.abstractHelicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections. © 2011 Elsevier B.V.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2011, v. 1813 n. 8, p. 1422-1427 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbamcr.2011.04.005
dc.identifier.citeulike9227500
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbamcr.2011.04.005
dc.identifier.epage1427
dc.identifier.hkuros189548
dc.identifier.hkuros211146
dc.identifier.isiWOS:000292945200004
dc.identifier.issn0167-4889
2011 Impact Factor: 5.538
2011 SCImago Journal Rankings: 0.707
dc.identifier.issue8
dc.identifier.pmid21539864
dc.identifier.scopuseid_2-s2.0-79959365764
dc.identifier.spage1422
dc.identifier.urihttp://hdl.handle.net/10722/137221
dc.identifier.volume1813
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
dc.publisher.placeNetherlands
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Research
dc.relation.referencesReferences in Scopus
dc.subject.meshBacterial Proteins - chemistry - physiology - ultrastructure
dc.subject.meshEpithelial Cells - microbiology - pathology
dc.subject.meshGastric Mucosa - microbiology - pathology
dc.subject.meshHelicobacter pylori - pathogenicity - physiology
dc.subject.meshProteins - chemistry - physiology - ultrastructure
dc.subjectAmyloid
dc.subjectGastric epithelial cell
dc.subjectHelicobacter pylori
dc.subjectHpn
dc.subjectMitochondria
dc.titleHistidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University
  3. Jinan University