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Article: Tracking bismuth antiulcer drug uptake in single helicobacter pylori cells

TitleTracking bismuth antiulcer drug uptake in single helicobacter pylori cells
Authors
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html
Citation
Journal Of The American Chemical Society, 2011, v. 133 n. 19, p. 7355-7357 How to Cite?
AbstractBismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and ∼2.9 × 107 Mg atoms/cell was determined for the wild-type. Bacteria treated with a Bi antiulcer drug deposited nearly 1.0 × 106 Bi atoms/cell, whereas the uptake process took ∼3 h to reach the half-maximum. Interference of ferric ions on bismuth uptake was demonstrated, suggesting that the metallodrug can utilize certain iron-transport pathways in the pathogen. The approach provides a general strategy for monitoring metals in single cells, facilitating exploration of metal-relevant bioprocesses. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/137219
ISSN
2015 Impact Factor: 13.038
2015 SCImago Journal Rankings: 7.123
ISI Accession Number ID
Funding AgencyGrant Number
Grants Council of Hong Kong SAR, P. R. ChinaHKU7043/06P
HKU1/07C
HKU7042/07P
HKU7006/09P
HKU7049/09P
N_HKU752/09
Croucher Foundation
Seed Funding Scheme for Basic Research of the University of Hong Kong
Funding Information:

This work was supported by the Research Grants Council of Hong Kong SAR, P. R. China (Projects HKU7043/06P, HKU1/07C, HKU7042/07P, HKU7006/09P, HKU7049/09P, and N_HKU752/09), the Croucher Foundation, and the Seed Funding Scheme for Basic Research of the University of Hong Kong. C.N.T. is grateful to F. Ng and Prof. B. J. Zheng from the LKS Faculty of Medicine, HKU, for assistance with H. pylori culture at the initial stage of the project.

References

 

DC FieldValueLanguage
dc.contributor.authorTsang, CNen_HK
dc.contributor.authorHo, KSen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorChan, WTen_HK
dc.date.accessioned2011-08-26T14:19:14Z-
dc.date.available2011-08-26T14:19:14Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of The American Chemical Society, 2011, v. 133 n. 19, p. 7355-7357en_HK
dc.identifier.issn0002-7863en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137219-
dc.description.abstractBismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and ∼2.9 × 107 Mg atoms/cell was determined for the wild-type. Bacteria treated with a Bi antiulcer drug deposited nearly 1.0 × 106 Bi atoms/cell, whereas the uptake process took ∼3 h to reach the half-maximum. Interference of ferric ions on bismuth uptake was demonstrated, suggesting that the metallodrug can utilize certain iron-transport pathways in the pathogen. The approach provides a general strategy for monitoring metals in single cells, facilitating exploration of metal-relevant bioprocesses. © 2011 American Chemical Society.en_HK
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.htmlen_HK
dc.relation.ispartofJournal of the American Chemical Societyen_HK
dc.subject.meshAnti-Ulcer Agents - pharmacokinetics-
dc.subject.meshBiological Transport-
dc.subject.meshBismuth - pharmacokinetics-
dc.subject.meshHelicobacter pylori - metabolism-
dc.titleTracking bismuth antiulcer drug uptake in single helicobacter pylori cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_HK
dc.identifier.emailChan, WT:wtchan@hku.hken_HK
dc.identifier.authoritySun, H=rp00777en_HK
dc.identifier.authorityChan, WT=rp00668en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/ja2013278en_HK
dc.identifier.pmid21517022-
dc.identifier.scopuseid_2-s2.0-79955881998en_HK
dc.identifier.hkuros189542en_US
dc.identifier.hkuros232301-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955881998&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume133en_HK
dc.identifier.issue19en_HK
dc.identifier.spage7355en_HK
dc.identifier.epage7357en_HK
dc.identifier.eissn1520-5126-
dc.identifier.isiWOS:000290782200031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTsang, CN=36624389600en_HK
dc.identifier.scopusauthoridHo, KS=36162334100en_HK
dc.identifier.scopusauthoridSun, H=7404827446en_HK
dc.identifier.scopusauthoridChan, WT=7403918827en_HK

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