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Article: Functional and sequence analysis of human neuroglobin gene promoter region

TitleFunctional and sequence analysis of human neuroglobin gene promoter region
Authors
KeywordsMethylation
Neuroglobin (NGB)
Promoter
Sp1
Sp3
Transcription start site
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713381/description?navopenmenu=-2
Citation
Biochimica Et Biophysica Acta - Gene Regulatory Mechanisms, 2011, v. 1809 n. 4-6, p. 236-244 How to Cite?
AbstractNeuroglobin (Ngb), a recently found oxygen-binding protein belonging to the vertebrate globin family, is mainly expressed in neurons of brains and eyes. Current studies have revealed diverse potential functions of Ngb and it was found to be able to reduce the severity of stroke and Alzheimer's disease, implying its importance in brains. However, the mechanism of Ngb regulation of transcription has not been elucidated yet. In this study, we analyzed the 5'-flanking region of human neuroglobin gene (NGB) and identified a transcription start site (TSS) located at -306. bp relative to the translation start site ATG. We characterized the proximal promoter of NGB and found two GC-boxes located at -16 and +. 30. bp relative to the TSS which are bound by transcription factor Sp1 and Sp3. Mutation of either GC-box led to a significant reduction in NGB promoter activity, while overexpression of Sp1 and Sp3 resulted in activation of the promoter. However, two putative NRSE sites (-359 and -127. bp relative to the TSS) apparently showed no influence on NGB tissue-specific expression. Treatment of two non-neuronal cell lines HeLa and BEAS-2B with 5-aza-2'-deoxycytidine remarkably induced NGB expression, suggesting a potential role of DNA methylation in regulating NGB tissue-specific expression. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/137217
ISSN
2015 Impact Factor: 5.373
2015 SCImago Journal Rankings: 4.460
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200811159093
Funding Information:

We thank Dr. Michael Antoniou (King's College, London) for his helpful suggestions on this study. This work was supported by Seed funding for basic research (200811159093), The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Wen_HK
dc.contributor.authorTian, Zen_HK
dc.contributor.authorSha, Sen_HK
dc.contributor.authorCheng, LYLen_HK
dc.contributor.authorPhilipsen, Sen_HK
dc.contributor.authorTanUn, KCen_HK
dc.date.accessioned2011-08-26T14:19:04Z-
dc.date.available2011-08-26T14:19:04Z-
dc.date.issued2011en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Regulatory Mechanisms, 2011, v. 1809 n. 4-6, p. 236-244en_HK
dc.identifier.issn1874-9399en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137217-
dc.description.abstractNeuroglobin (Ngb), a recently found oxygen-binding protein belonging to the vertebrate globin family, is mainly expressed in neurons of brains and eyes. Current studies have revealed diverse potential functions of Ngb and it was found to be able to reduce the severity of stroke and Alzheimer's disease, implying its importance in brains. However, the mechanism of Ngb regulation of transcription has not been elucidated yet. In this study, we analyzed the 5'-flanking region of human neuroglobin gene (NGB) and identified a transcription start site (TSS) located at -306. bp relative to the translation start site ATG. We characterized the proximal promoter of NGB and found two GC-boxes located at -16 and +. 30. bp relative to the TSS which are bound by transcription factor Sp1 and Sp3. Mutation of either GC-box led to a significant reduction in NGB promoter activity, while overexpression of Sp1 and Sp3 resulted in activation of the promoter. However, two putative NRSE sites (-359 and -127. bp relative to the TSS) apparently showed no influence on NGB tissue-specific expression. Treatment of two non-neuronal cell lines HeLa and BEAS-2B with 5-aza-2'-deoxycytidine remarkably induced NGB expression, suggesting a potential role of DNA methylation in regulating NGB tissue-specific expression. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713381/description?navopenmenu=-2en_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Regulatory Mechanismsen_HK
dc.subjectMethylationen_HK
dc.subjectNeuroglobin (NGB)en_HK
dc.subjectPromoteren_HK
dc.subjectSp1en_HK
dc.subjectSp3en_HK
dc.subjectTranscription start siteen_HK
dc.subject.mesh5' Flanking Region - geneticsen_HK
dc.subject.meshAzacitidine - analogs & derivatives - pharmacologyen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBinding Sites - geneticsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromatin Immunoprecipitationen_HK
dc.subject.meshElectrophoretic Mobility Shift Assayen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshGlobins - genetics - metabolismen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLuciferases - genetics - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMutagenesis, Site-Directeden_HK
dc.subject.meshNerve Tissue Proteins - genetics - metabolismen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshRegulatory Sequences, Nucleic Acid - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSp1 Transcription Factor - metabolismen_HK
dc.subject.meshSp3 Transcription Factor - metabolismen_HK
dc.subject.meshTranscription Initiation Siteen_HK
dc.titleFunctional and sequence analysis of human neuroglobin gene promoter regionen_HK
dc.typeArticleen_HK
dc.identifier.emailCheng, LYL: lcheng@hkucc.hku.hken_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityCheng, LYL=rp00317en_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbagrm.2011.02.003en_HK
dc.identifier.pmid21362510-
dc.identifier.scopuseid_2-s2.0-79957788312en_HK
dc.identifier.hkuros192006en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957788312&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1809en_HK
dc.identifier.issue4-6en_HK
dc.identifier.spage236en_HK
dc.identifier.epage244en_HK
dc.identifier.isiWOS:000291900200003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, W=36066941200en_HK
dc.identifier.scopusauthoridTian, Z=49461844800en_HK
dc.identifier.scopusauthoridSha, S=49461779600en_HK
dc.identifier.scopusauthoridCheng, LYL=7403337122en_HK
dc.identifier.scopusauthoridPhilipsen, S=7003745052en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK

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