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Article: The expression and roles of inhibitor of DNA binding helix-loop-helix proteins in the developing and adult mouse retina

TitleThe expression and roles of inhibitor of DNA binding helix-loop-helix proteins in the developing and adult mouse retina
Authors
KeywordsDevelopment
Differentiation
Retinal neurons
Transcription factor
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 2011, v. 175, p. 367-379 How to Cite?
AbstractInhibitor of DNA binding (Id) proteins bind to and inhibit the function of basic helix-loop-helix (bHLH) transcription factors including those that regulate retinal development. However, little is known about the role of Id proteins in the growth and differentiation of the retina during development. The purpose of this study is to observe the expression of Id proteins in the developing and adult mouse retinas as the first step in investigating the functions of Id family members in the eye. The expression of Id1-4 was examined by real-time PCR, Western blot, and immunohistochemistry in wild-type and Id1/Id3 double-knockout mice. Id1-4 genes and proteins showed high expression levels in the retina at embryonic and early postnatal stages, whereas declined in the adult. Expression of Id proteins was observed in the inner neuroblastic layer (NBL) at embryonic (E) day 13.5 through 16.5. Id4 expression began at E18.5. By E18.5 and postnatal day 1, the expression of Id1-4 exhibited distinct yet overlapping patterns in the ganglion cell layer and inner part of NBL. In the adult, Ids were expressed in retinal ganglion cells, amacrine cells, bipolar cells, and horizontal cells. No Id expression was found in Müller cells. Id1 and Id3 double-knockout mice (Id1-/-/Id3-/-) showed smaller retinal size compared to wild-type or heterozygous littermates. However, histological analyses in Id1 and Id3 single-knockout retinas revealed no obvious defects in developmental phenotype. Our results indicate that the expression of the Id family may play an important role in regulating retinal progenitor cell proliferation and differentiation. © 2011 IBRO.
Persistent Identifierhttp://hdl.handle.net/10722/137206
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200611159203
Regional Grant Council of Hong Kong10208603
Funding Information:

We thank Dr. R. Benezar for the Id1/Id3 heterozygous knockout mice, E. Campbell for editing of the manuscript, and Dr. T.H. Chu for the Photoshop work. This study was supported by grants from the University of Hong Kong Seed Funding Program for Basic Research (No. 200611159203) and General Research Fund (No. 10208603) from the Regional Grant Council of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorDu, Yen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2011-08-26T14:18:47Z-
dc.date.available2011-08-26T14:18:47Z-
dc.date.issued2011en_HK
dc.identifier.citationNeuroscience, 2011, v. 175, p. 367-379en_HK
dc.identifier.issn0306-4522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137206-
dc.description.abstractInhibitor of DNA binding (Id) proteins bind to and inhibit the function of basic helix-loop-helix (bHLH) transcription factors including those that regulate retinal development. However, little is known about the role of Id proteins in the growth and differentiation of the retina during development. The purpose of this study is to observe the expression of Id proteins in the developing and adult mouse retinas as the first step in investigating the functions of Id family members in the eye. The expression of Id1-4 was examined by real-time PCR, Western blot, and immunohistochemistry in wild-type and Id1/Id3 double-knockout mice. Id1-4 genes and proteins showed high expression levels in the retina at embryonic and early postnatal stages, whereas declined in the adult. Expression of Id proteins was observed in the inner neuroblastic layer (NBL) at embryonic (E) day 13.5 through 16.5. Id4 expression began at E18.5. By E18.5 and postnatal day 1, the expression of Id1-4 exhibited distinct yet overlapping patterns in the ganglion cell layer and inner part of NBL. In the adult, Ids were expressed in retinal ganglion cells, amacrine cells, bipolar cells, and horizontal cells. No Id expression was found in Müller cells. Id1 and Id3 double-knockout mice (Id1-/-/Id3-/-) showed smaller retinal size compared to wild-type or heterozygous littermates. However, histological analyses in Id1 and Id3 single-knockout retinas revealed no obvious defects in developmental phenotype. Our results indicate that the expression of the Id family may play an important role in regulating retinal progenitor cell proliferation and differentiation. © 2011 IBRO.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_HK
dc.relation.ispartofNeuroscienceen_HK
dc.subjectDevelopmenten_HK
dc.subjectDifferentiationen_HK
dc.subjectRetinal neuronsen_HK
dc.subjectTranscription factoren_HK
dc.titleThe expression and roles of inhibitor of DNA binding helix-loop-helix proteins in the developing and adult mouse retinaen_HK
dc.typeArticleen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neuroscience.2010.12.007en_HK
dc.identifier.pmid21145943-
dc.identifier.scopuseid_2-s2.0-78951486126en_HK
dc.identifier.hkuros191953en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78951486126&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume175en_HK
dc.identifier.spage367en_HK
dc.identifier.epage379en_HK
dc.identifier.eissn1873-7544-
dc.identifier.isiWOS:000287106300034-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridDu, Y=36993717500en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.citeulike8449170-

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