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Article: The antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro

TitleThe antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitro
Authors
KeywordsAntimicrobial
Fructus mume
Orthodontic
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2011, v. 56 n. 1, p. 16-21 How to Cite?
AbstractObjective: The aim of this study was to evaluate the antimicrobial efficacy of Traditional Chinese Medicine Fructus mume on a monospecies-biofilm model established on orthodontic brackets in vitro. Materials and methods: The antimicrobial effect of Fructus mume aqueous extract on the planktonic Streptococcus mutans (S. mutans) was tested by microdilution method (MIC). The cell viability of S. mutans biofilm on Damon3 MX bracket (Ormco, USA) after exposed to Fructus mume extract was quantified by XTT reduction assay. Visualization of the samples was performed by fluorescence microscope and confocal laser scanning microscopy (CLSM). Results: HPLC analysis revealed that the main compounds of Fructus mume are organic acids. The MIC of Fructus mume extract on the planktonic S. mutans was 50 mg/mL. The optical density (OD) values, measured by XTT reduction assay from S. mutans biofilms after 1-min exposure to different test agents, demonstrated that the cell viability of S. mutans biofilms exposed to 250 mg/mL Fructus mume extract < BHI (-) (p < 0.01). Microscope image showed that Fructus mume extract obviously increased the amount of dead bacteria on the surface of bracket. Conclusion: Fructus mume extract showed antimicrobial effect on S. mutans biofilm on orthodontic bracket in vitro which may indicate its potential use as an oral antimicrobial agent for orthodontic patients. © 2010 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/137187
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
Funding AgencyGrant Number
International Association for Dental Research/GlaxoSmithKline
Funding Information:

Funding: This study was supported by the 2008 Innovation in Oral Care Awards (International Association for Dental Research/GlaxoSmithKline).

References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWong, RWKen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorHägg, Uen_HK
dc.contributor.authorMcGrath, Cen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorKao, Ren_HK
dc.date.accessioned2011-08-26T14:18:09Z-
dc.date.available2011-08-26T14:18:09Z-
dc.date.issued2011en_HK
dc.identifier.citationArchives Of Oral Biology, 2011, v. 56 n. 1, p. 16-21en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137187-
dc.description.abstractObjective: The aim of this study was to evaluate the antimicrobial efficacy of Traditional Chinese Medicine Fructus mume on a monospecies-biofilm model established on orthodontic brackets in vitro. Materials and methods: The antimicrobial effect of Fructus mume aqueous extract on the planktonic Streptococcus mutans (S. mutans) was tested by microdilution method (MIC). The cell viability of S. mutans biofilm on Damon3 MX bracket (Ormco, USA) after exposed to Fructus mume extract was quantified by XTT reduction assay. Visualization of the samples was performed by fluorescence microscope and confocal laser scanning microscopy (CLSM). Results: HPLC analysis revealed that the main compounds of Fructus mume are organic acids. The MIC of Fructus mume extract on the planktonic S. mutans was 50 mg/mL. The optical density (OD) values, measured by XTT reduction assay from S. mutans biofilms after 1-min exposure to different test agents, demonstrated that the cell viability of S. mutans biofilms exposed to 250 mg/mL Fructus mume extract < BHI (-) (p < 0.01). Microscope image showed that Fructus mume extract obviously increased the amount of dead bacteria on the surface of bracket. Conclusion: Fructus mume extract showed antimicrobial effect on S. mutans biofilm on orthodontic bracket in vitro which may indicate its potential use as an oral antimicrobial agent for orthodontic patients. © 2010 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Archives of Oral Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Archives of Oral Biology, 2011, v. 56 n. 1, p. 16-21. DOI: 10.1016/j.archoralbio.2010.08.006-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAntimicrobialen_HK
dc.subjectFructus mumeen_HK
dc.subjectOrthodonticen_HK
dc.subject.meshAnti-Bacterial Agents - pharmacologyen_US
dc.subject.meshBiofilms - drug effectsen_US
dc.subject.meshDrugs, Chinese Herbal - chemistry - pharmacologyen_US
dc.subject.meshOrthodontic Brackets - microbiologyen_US
dc.subject.meshPlant Extracts - analysis - pharmacologyen_US
dc.titleThe antimicrobial efficacy of Fructus mume extract on orthodontic bracket: A monospecies-biofilm model study in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=56&issue=1&spage=16&epage=21&date=2011&atitle=The+antimicrobial+efficacy+of+Fructus+mume+extract+on+orthodontic+bracket:+a+monospecies-biofilm+model+study+in+vitroen_US
dc.identifier.emailWong, RWK:fyoung@hkucc.hku.hken_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailHägg, U:euohagg@hkusua.hku.hken_HK
dc.identifier.emailMcGrath, C:mcgrathc@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailKao, R:rytkao@hkucc.hku.hken_HK
dc.identifier.authorityWong, RWK=rp00038en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authorityHägg, U=rp00020en_HK
dc.identifier.authorityMcGrath, C=rp00037en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityKao, R=rp00481en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.archoralbio.2010.08.006en_HK
dc.identifier.pmid20864088-
dc.identifier.scopuseid_2-s2.0-78650176307en_HK
dc.identifier.hkuros191874en_US
dc.identifier.hkuros183783-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650176307&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue1en_HK
dc.identifier.spage16en_HK
dc.identifier.epage21en_HK
dc.identifier.isiWOS:000286548400002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, Y=37116729600en_HK
dc.identifier.scopusauthoridWong, RWK=7402127170en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridHägg, U=7006790279en_HK
dc.identifier.scopusauthoridMcGrath, C=7102335507en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridKao, R=7101675499en_HK
dc.identifier.citeulike7890053-

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