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Article: Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms

TitleEffects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms
Authors
Issue Date2012
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439
Citation
International Journal Of Paediatric Dentistry, 2012, v. 22 n. 1, p. 2-10 How to Cite?
AbstractBackground. Silver diamine fluoride (SDF) has been shown to be a successful treatment for arresting caries. However, the mechanism of SDF is to be elucidated. Aim. To characterize the effects of SDF on dentine carious induced by Streptococcus mutans and Actinomyces naeslundii. Design. Thirty-two artificially demineralized human dentine blocks were inoculated: 16 with S. mutans and 16 with A. naeslundii. Either SDF or water was applied to eight blocks in each group. Biofilm morphology, microbial kinetics and viability were evaluated by scanning electron microscopy, colony forming units, and confocal microscopy. The crosssection of the dentine carious lesions were assessed by microhardness testing, scanning electron microscopy with energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. Results. Biofilm counts were reduced in SDF group than control (P<0.01). Surfaces of carious lesions were harder after SDF application than after water application (P<0.05), in S. mutans group, Ca and P weight percentage after SDF application than after water application (P<0.05). Lesions showed a significantly reduced level of matrix to phosphate after SDF treatment (P<0.05). Conclusion. Present study showed that SDF posses an anti-microbial activity against cariogenic biofilm of S. mutans or A. naeslundii formed on dentine surfaces. SDF slowed down demineralization of dentine. This dual activity could be the reason behind clinical success of SDF. © 2011 The Authors. International Journal of Paediatric Dentistry © 2011 BSPD, IAPD and Blackwell Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/137184
ISSN
2015 Impact Factor: 1.303
2015 SCImago Journal Rankings: 0.721
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200802159003
Funding Information:

The authors thank Ms Joyce Yau for assistance in the microbiological work, Mr Simon Lee for support in the SEM study, Dr Borong Shi for assistance in FTIR analysis, and Dr Trevor Lane for editorial assistance. This study was supported by Grant No. 200802159003 of the Seed Fund for Basic Research, The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChu, CHen_HK
dc.contributor.authorMei, Len_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorLo, ECMen_HK
dc.date.accessioned2011-08-26T14:18:07Z-
dc.date.available2011-08-26T14:18:07Z-
dc.date.issued2012en_HK
dc.identifier.citationInternational Journal Of Paediatric Dentistry, 2012, v. 22 n. 1, p. 2-10en_HK
dc.identifier.issn0960-7439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137184-
dc.description.abstractBackground. Silver diamine fluoride (SDF) has been shown to be a successful treatment for arresting caries. However, the mechanism of SDF is to be elucidated. Aim. To characterize the effects of SDF on dentine carious induced by Streptococcus mutans and Actinomyces naeslundii. Design. Thirty-two artificially demineralized human dentine blocks were inoculated: 16 with S. mutans and 16 with A. naeslundii. Either SDF or water was applied to eight blocks in each group. Biofilm morphology, microbial kinetics and viability were evaluated by scanning electron microscopy, colony forming units, and confocal microscopy. The crosssection of the dentine carious lesions were assessed by microhardness testing, scanning electron microscopy with energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. Results. Biofilm counts were reduced in SDF group than control (P<0.01). Surfaces of carious lesions were harder after SDF application than after water application (P<0.05), in S. mutans group, Ca and P weight percentage after SDF application than after water application (P<0.05). Lesions showed a significantly reduced level of matrix to phosphate after SDF treatment (P<0.05). Conclusion. Present study showed that SDF posses an anti-microbial activity against cariogenic biofilm of S. mutans or A. naeslundii formed on dentine surfaces. SDF slowed down demineralization of dentine. This dual activity could be the reason behind clinical success of SDF. © 2011 The Authors. International Journal of Paediatric Dentistry © 2011 BSPD, IAPD and Blackwell Publishing Ltd.en_HK
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439en_HK
dc.relation.ispartofInternational Journal of Paediatric Dentistryen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.comen_US
dc.subject.meshActinomyces - drug effects-
dc.subject.meshCariostatic Agents - pharmacology-
dc.subject.meshDental Caries - microbiology - pathology - prevention and control-
dc.subject.meshDentin - drug effects - microbiology - pathology - ultrastructure-
dc.subject.meshQuaternary Ammonium Compounds - pharmacology-
dc.titleEffects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.emailChu, CH:chchu@hku.hken_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailLo, ECM:hrdplcm@hkucc.hku.hken_HK
dc.identifier.authorityChu, CH=rp00022en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authorityLo, ECM=rp00015en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1365-263X.2011.01149.xen_HK
dc.identifier.pmid21702854-
dc.identifier.scopuseid_2-s2.0-83555166203en_HK
dc.identifier.hkuros191870en_US
dc.identifier.hkuros200147-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83555166203&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue1en_HK
dc.identifier.spage2en_HK
dc.identifier.epage10en_HK
dc.identifier.eissn1365-263X-
dc.identifier.isiWOS:000297954300002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChu, CH=7404345729en_HK
dc.identifier.scopusauthoridMei, L=54780178100en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridLo, ECM=7101705982en_HK

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