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Article: Differential Phytate Utilization in Candida species

TitleDifferential Phytate Utilization in Candida species
Authors
KeywordsCandida
Phytase
Phytate hydrolysis
Issue Date2011
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2011, v. 172 n. 6, p. 473-479 How to Cite?
AbstractThe present study was undertaken to evaluate and characterize the phytase activity in different Candida species. A total of 113 Candida isolates representing eight species were examined for phytase activity by an agar plate assay using the calcium salt of phytic acid as the sole phosphorus source. A phytase-positive phenotype was identified by the formation of a clear halo around a fungal colony. Cell-bound differential phytase activity was observed in Candida isolates at inter- and intra-species levels. Although phytase activity was not affected by the supplementation of external phosphate in C. albicans, C. dubliniensis, C. glabrata, and C. kefyr, elevated phytase activity was evident in C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis in phosphate-free medium. Further characterization showed that, in general, relatively higher phytase activity was observed at more acidic pHs, and the phytase activity increased with incubation temperature, reaching a maximum at 55 or 65°C. Taken together, the findings demonstrated, for the first time, differential phytase activities in different Candida species. Phytase activity may be a contributing factor to fungal survival and proliferation within the human gastrointestinal tract, where nutrients are usually scarce. © 2011 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/137180
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong201003159008
Funding Information:

The research was supported by the Seed Funding Programme for Basic Research from the University of Hong Kong (Project No. 201003159008). The author thanks Prof. L. P. Samaranayake (The University of Hong Kong) for providing some of the Candida strains, Dr. Trevor Lane for editorial assistance, and Mrs. P. W. Y. Ho for helpful discussion.

References

 

DC FieldValueLanguage
dc.contributor.authorTsang, PWKen_HK
dc.date.accessioned2011-08-26T14:18:04Z-
dc.date.available2011-08-26T14:18:04Z-
dc.date.issued2011en_HK
dc.identifier.citationMycopathologia, 2011, v. 172 n. 6, p. 473-479en_HK
dc.identifier.issn0301-486Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/137180-
dc.description.abstractThe present study was undertaken to evaluate and characterize the phytase activity in different Candida species. A total of 113 Candida isolates representing eight species were examined for phytase activity by an agar plate assay using the calcium salt of phytic acid as the sole phosphorus source. A phytase-positive phenotype was identified by the formation of a clear halo around a fungal colony. Cell-bound differential phytase activity was observed in Candida isolates at inter- and intra-species levels. Although phytase activity was not affected by the supplementation of external phosphate in C. albicans, C. dubliniensis, C. glabrata, and C. kefyr, elevated phytase activity was evident in C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis in phosphate-free medium. Further characterization showed that, in general, relatively higher phytase activity was observed at more acidic pHs, and the phytase activity increased with incubation temperature, reaching a maximum at 55 or 65°C. Taken together, the findings demonstrated, for the first time, differential phytase activities in different Candida species. Phytase activity may be a contributing factor to fungal survival and proliferation within the human gastrointestinal tract, where nutrients are usually scarce. © 2011 Springer Science+Business Media B.V.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_HK
dc.relation.ispartofMycopathologiaen_HK
dc.rightsThe original publication is available at www.springerlink.comen_US
dc.subjectCandidaen_HK
dc.subjectPhytaseen_HK
dc.subjectPhytate hydrolysisen_HK
dc.titleDifferential Phytate Utilization in Candida speciesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-486X&volume=172&issue=6&spage=473&epage=479&date=2011&atitle=Differential+phytate+utilization+in+Candida+species-
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11046-011-9453-3en_HK
dc.identifier.pmid21792623-
dc.identifier.scopuseid_2-s2.0-80855138664en_HK
dc.identifier.hkuros191021en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80855138664&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume172en_HK
dc.identifier.issue6en_HK
dc.identifier.spage473en_HK
dc.identifier.epage479en_HK
dc.identifier.isiWOS:000297626300007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.citeulike9668385-

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