File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of dental pulp stem cells isolated from canine premolars

TitleCharacterization of dental pulp stem cells isolated from canine premolars
Authors
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com
Citation
The Journal of Endodontics, 2011, v. 37 n. 8, p. 1074-1080 How to Cite?
AbstractINTRODUCTION: Dogs are commonly used animal models for regenerative endodontics research. Although several studies have used stem cells isolated from dog teeth to investigate the dentin/pulp regeneration in vivo, less attention has been paid for the characterization of these cells. Therefore, this study aimed to characterize the dental pulp stem cells isolated from dog teeth (cDPSCs) in order to further define the dog as an animal model for regenerative endodontics. METHODS: Stem cells were isolated from freshly extracted premolars of 10-month-old Beagles. The isolated cells were investigated for their stem cell properties by analysis of their clonogenic and growth characteristics; expression of mesenchymal stem cell markers; and evaluation of their osteo/odontogenic, adipogenic, and neurogenic potential. RESULTS: A colony formation assay showed the existence of a clonogenic cell population in cDPSCs isolated. The growth curves revealed a higher proliferation rate of cDPSCs compared with hBMMSCs. cDPSCs expressed mesenchymal stem cell markers STRO-1, CD146, and Nanog. However, they were negative for CD73, CD105, and CD45. cDPSCs were able to differentiate into odontoblast-like cells as shown by increased alkaline phosphatase activity, dentin sialoprotein expression, and formation of mineralized nodules. cDPSCs also showed the neurogenic and adipogenic differentiation potential at a lower level compared with those of hDPSCs and hBMMSCs. CONCLUSIONS: The results of this study confirmed the stem cell properties of cDPSCs at a comparable level to those of hDPSCs and hBMMSCs. Overall, the data presented in this study provide evidence in supportive of using cDPSCs and dogs as an animal model in dental tissue engineering via stem cell-based approaches.
Persistent Identifierhttp://hdl.handle.net/10722/137179
ISSN
2015 Impact Factor: 4.498
2015 SCImago Journal Rankings: 1.910
ISI Accession Number ID
Funding AgencyGrant Number
The University of Hong Kong, Hong Kong SAR, China200910159002
International Cooperation in Science and Technology Cooperation Project, Beijing, China2009DFA32950
Funding Information:

This work was supported by grants from the Seed Funding Programme for Basic Research of The University of Hong Kong, Hong Kong SAR, China (grant number 200910159002) and from the International Cooperation in Science and Technology Cooperation Project, Beijing, China (grant number 2009DFA32950).

References

 

DC FieldValueLanguage
dc.contributor.authorDissanayaka, WLen_HK
dc.contributor.authorZhu, Xen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2011-08-26T14:18:04Z-
dc.date.available2011-08-26T14:18:04Z-
dc.date.issued2011en_HK
dc.identifier.citationThe Journal of Endodontics, 2011, v. 37 n. 8, p. 1074-1080en_HK
dc.identifier.issn0022-0795en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137179-
dc.description.abstractINTRODUCTION: Dogs are commonly used animal models for regenerative endodontics research. Although several studies have used stem cells isolated from dog teeth to investigate the dentin/pulp regeneration in vivo, less attention has been paid for the characterization of these cells. Therefore, this study aimed to characterize the dental pulp stem cells isolated from dog teeth (cDPSCs) in order to further define the dog as an animal model for regenerative endodontics. METHODS: Stem cells were isolated from freshly extracted premolars of 10-month-old Beagles. The isolated cells were investigated for their stem cell properties by analysis of their clonogenic and growth characteristics; expression of mesenchymal stem cell markers; and evaluation of their osteo/odontogenic, adipogenic, and neurogenic potential. RESULTS: A colony formation assay showed the existence of a clonogenic cell population in cDPSCs isolated. The growth curves revealed a higher proliferation rate of cDPSCs compared with hBMMSCs. cDPSCs expressed mesenchymal stem cell markers STRO-1, CD146, and Nanog. However, they were negative for CD73, CD105, and CD45. cDPSCs were able to differentiate into odontoblast-like cells as shown by increased alkaline phosphatase activity, dentin sialoprotein expression, and formation of mineralized nodules. cDPSCs also showed the neurogenic and adipogenic differentiation potential at a lower level compared with those of hDPSCs and hBMMSCs. CONCLUSIONS: The results of this study confirmed the stem cell properties of cDPSCs at a comparable level to those of hDPSCs and hBMMSCs. Overall, the data presented in this study provide evidence in supportive of using cDPSCs and dogs as an animal model in dental tissue engineering via stem cell-based approaches.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.jendodon.comen_HK
dc.relation.ispartofThe Journal of Endodonticsen_HK
dc.subject.meshAdult Stem Cellsen_HK
dc.subject.meshDental Pulp - cytologyen_HK
dc.subject.meshMesenchymal Stem Cells - metabolismen_HK
dc.subject.meshOdontoblasts - cytology - metabolismen_HK
dc.subject.meshTissue Engineeringen_HK
dc.titleCharacterization of dental pulp stem cells isolated from canine premolarsen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, C: zhangcf@hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_HK
dc.identifier.authorityZhang, C=rp01408en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.joen.2011.04.004en_HK
dc.identifier.pmid21763897-
dc.identifier.scopuseid_2-s2.0-79960471046en_HK
dc.identifier.hkuros190411en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960471046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1074en_HK
dc.identifier.epage1080en_HK
dc.identifier.eissn1878-3554-
dc.identifier.isiWOS:000294091500007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridZhang, C=7405494609en_HK
dc.identifier.scopusauthoridZhu, X=36659978300en_HK
dc.identifier.scopusauthoridDissanayaka, WL=36196419000en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats