File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
  • Basic View
  • Metadata View
  • XML View
TitlePorphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
 
AuthorsHerath, TDK2
Wang, Y2
Seneviratne, CJ2
Lu, Q2
Darveau, RP3
Wang, CY1
Jin, L2
 
KeywordsCytokines
Human gingival fibroblasts
Lipopolysaccharide
Periodontal disease
Porphyromonas gingivalis
 
Issue Date2011
 
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
CitationJournal of Clinical Periodontology, 2011, v. 38 n. 8, p. 694-701 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2011.01741.x
 
AbstractAim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). Materials and methods: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.
 
ISSN0303-6979
2013 Impact Factor: 3.610
 
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2011.01741.x
 
ISI Accession Number IDWOS:000292693000002
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU766909M
International Association for Dental Research (IADR)/Lion Dental Research
Funding Information:

This study was supported by the Hong Kong Research Grants Council (HKU766909M to LJJ).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHerath, TDK
 
dc.contributor.authorWang, Y
 
dc.contributor.authorSeneviratne, CJ
 
dc.contributor.authorLu, Q
 
dc.contributor.authorDarveau, RP
 
dc.contributor.authorWang, CY
 
dc.contributor.authorJin, L
 
dc.date.accessioned2011-08-26T14:18:00Z
 
dc.date.available2011-08-26T14:18:00Z
 
dc.date.issued2011
 
dc.description.abstractAim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). Materials and methods: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal of Clinical Periodontology, 2011, v. 38 n. 8, p. 694-701 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2011.01741.x
 
dc.identifier.citeulike9552013
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2011.01741.x
 
dc.identifier.epage701
 
dc.identifier.hkuros189288
 
dc.identifier.isiWOS:000292693000002
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU766909M
International Association for Dental Research (IADR)/Lion Dental Research
Funding Information:

This study was supported by the Hong Kong Research Grants Council (HKU766909M to LJJ).

 
dc.identifier.issn0303-6979
2013 Impact Factor: 3.610
 
dc.identifier.issue8
 
dc.identifier.openurl
 
dc.identifier.pmid21752043
 
dc.identifier.scopuseid_2-s2.0-79960369022
 
dc.identifier.spage694
 
dc.identifier.urihttp://hdl.handle.net/10722/137172
 
dc.identifier.volume38
 
dc.languageeng
 
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
dc.publisher.placeDenmark
 
dc.relation.ispartofJournal of Clinical Periodontology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe definitive version is available at www.blackwell-synergy.com
 
dc.subjectCytokines
 
dc.subjectHuman gingival fibroblasts
 
dc.subjectLipopolysaccharide
 
dc.subjectPeriodontal disease
 
dc.subjectPorphyromonas gingivalis
 
dc.titlePorphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Herath, TDK</contributor.author>
<contributor.author>Wang, Y</contributor.author>
<contributor.author>Seneviratne, CJ</contributor.author>
<contributor.author>Lu, Q</contributor.author>
<contributor.author>Darveau, RP</contributor.author>
<contributor.author>Wang, CY</contributor.author>
<contributor.author>Jin, L</contributor.author>
<date.accessioned>2011-08-26T14:18:00Z</date.accessioned>
<date.available>2011-08-26T14:18:00Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>Journal of Clinical Periodontology, 2011, v. 38 n. 8, p. 694-701</identifier.citation>
<identifier.issn>0303-6979</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/137172</identifier.uri>
<description.abstract>Aim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-&#945; in human gingival fibroblasts (HGFs). Materials and methods: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1&#8195;ng-10&#8195;&#956;g/ml) and time-dependent (2-48&#8195;h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-&#945; transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-&#945; transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2&#8195;h of stimulation and gradually declined afterwards. Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.</description.abstract>
<language>eng</language>
<publisher>Blackwell Munksgaard. The Journal&apos;s web site is located at http://www.blackwellpublishing.com/journals/CPE</publisher>
<relation.ispartof>Journal of Clinical Periodontology</relation.ispartof>
<rights>The definitive version is available at www.blackwell-synergy.com</rights>
<subject>Cytokines</subject>
<subject>Human gingival fibroblasts</subject>
<subject>Lipopolysaccharide</subject>
<subject>Periodontal disease</subject>
<subject>Porphyromonas gingivalis</subject>
<title>Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0303-6979&amp;volume=38&amp;issue=8&amp;spage=694&amp;epage=701&amp;date=2011&amp;atitle=Porphyromonas+gingivalis+lipopolysaccharide+lipid+A+heterogeneity+differentially+modulates+the+expression+of+IL-6+and+IL-8+in+human+gingival+fibroblasts</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1111/j.1600-051X.2011.01741.x</identifier.doi>
<identifier.pmid>21752043</identifier.pmid>
<identifier.scopus>eid_2-s2.0-79960369022</identifier.scopus>
<identifier.hkuros>189288</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-79960369022&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>38</identifier.volume>
<identifier.issue>8</identifier.issue>
<identifier.spage>694</identifier.spage>
<identifier.epage>701</identifier.epage>
<identifier.isi>WOS:000292693000002</identifier.isi>
<publisher.place>Denmark</publisher.place>
<identifier.citeulike>9552013</identifier.citeulike>
</item>
Author Affiliations
  1. The UCLA School of Dentistry
  2. The University of Hong Kong
  3. University of Washington Seattle