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Article: Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts

TitlePorphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
Authors
KeywordsCytokines
Human gingival fibroblasts
Lipopolysaccharide
Periodontal disease
Porphyromonas gingivalis
Issue Date2011
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal of Clinical Periodontology, 2011, v. 38 n. 8, p. 694-701 How to Cite?
AbstractAim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). Materials and methods: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/137172
ISSN
2014 Impact Factor: 4.010
2014 SCImago Journal Rankings: 1.714
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU766909M
International Association for Dental Research (IADR)/Lion Dental Research
Funding Information:

This study was supported by the Hong Kong Research Grants Council (HKU766909M to LJJ).

References

 

DC FieldValueLanguage
dc.contributor.authorHerath, TDKen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorLu, Qen_HK
dc.contributor.authorDarveau, RPen_HK
dc.contributor.authorWang, CYen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2011-08-26T14:18:00Z-
dc.date.available2011-08-26T14:18:00Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal of Clinical Periodontology, 2011, v. 38 n. 8, p. 694-701en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137172-
dc.description.abstractAim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra- (LPS(1435/1449) ) and penta-acylated (LPS(1690) ) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-α in human gingival fibroblasts (HGFs). Materials and methods: HGFs were stimulated with P. gingivalis LPS(1435/1449) and LPS(1690) in both dose- (1 ng-10 μg/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-α transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: P. gingivalis LPS(1690) significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS(1435/1449) did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS(1690) continuously increased with time course. In contrast, TNF-α transcript expression was up-regulated promptly by P. gingivalis LPS(1690) after 2 h of stimulation and gradually declined afterwards. Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.en_HK
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_US
dc.subjectCytokinesen_HK
dc.subjectHuman gingival fibroblastsen_HK
dc.subjectLipopolysaccharideen_HK
dc.subjectPeriodontal diseaseen_HK
dc.subjectPorphyromonas gingivalisen_HK
dc.titlePorphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblastsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=38&issue=8&spage=694&epage=701&date=2011&atitle=Porphyromonas+gingivalis+lipopolysaccharide+lipid+A+heterogeneity+differentially+modulates+the+expression+of+IL-6+and+IL-8+in+human+gingival+fibroblastsen_US
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-051X.2011.01741.xen_HK
dc.identifier.pmid21752043en_HK
dc.identifier.scopuseid_2-s2.0-79960369022en_HK
dc.identifier.hkuros189288en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960369022&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue8en_HK
dc.identifier.spage694en_HK
dc.identifier.epage701en_HK
dc.identifier.isiWOS:000292693000002-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridWang, CY=35276383300en_HK
dc.identifier.scopusauthoridDarveau, RP=16300829400en_HK
dc.identifier.scopusauthoridLu, Q=36128876000en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridWang, Y=45061096800en_HK
dc.identifier.scopusauthoridHerath, TDK=40561255100en_HK
dc.identifier.citeulike9552013-

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