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Article: Human Yip1A specifies the localization of Yif1 to the Golgi apparatus

TitleHuman Yip1A specifies the localization of Yif1 to the Golgi apparatus
Authors
KeywordsSpecies Index: Saccharomycetales
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2005, v. 334 n. 1, p. 16-22 How to Cite?
AbstractYip1p and Yif1p are essential for transport from ER to Golgi stack during the early secretory pathway in budding yeast. Here, we report the identification and characterization of human Yif1. Sequence analysis revealed that human Yif1 (HsYif1), like most of the other YIP1 protein family members, contains multiple transmembrane segments. Double immunofluorescence study revealed co-distribution of HsYif1 with Golgi marker such as GS27. To delineate the function of HsYif1, we conducted a yeast two-hybrid assay and identified an interaction between human HsYif1 and HsYip1A, a homolog of yeast Yip1. In addition, our immunoprecipitation pull-down assay validates the interaction between HsYif1 and HsYip1A. Moreover, our immunofluorescence study demonstrates the co-distribution of HsYif1 and HsYip1A. Significantly, over-expression of mutant HsYip1A-lacked cytosolic region disrupts the localization of HsYif1 to the Golgi, suggesting that HsYip1A specifies the localization of HsYif1 to the Golgi. Therefore, we conclude that human Yip1A interacts with and determines the localization of HsYif1 to the Golgi apparatus. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/136782
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Cen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorZhu, Hen_HK
dc.contributor.authorAhmed, Ken_HK
dc.contributor.authorFu, Cen_HK
dc.contributor.authorYao, Xen_HK
dc.date.accessioned2011-07-29T02:12:09Z-
dc.date.available2011-07-29T02:12:09Z-
dc.date.issued2005en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2005, v. 334 n. 1, p. 16-22en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/136782-
dc.description.abstractYip1p and Yif1p are essential for transport from ER to Golgi stack during the early secretory pathway in budding yeast. Here, we report the identification and characterization of human Yif1. Sequence analysis revealed that human Yif1 (HsYif1), like most of the other YIP1 protein family members, contains multiple transmembrane segments. Double immunofluorescence study revealed co-distribution of HsYif1 with Golgi marker such as GS27. To delineate the function of HsYif1, we conducted a yeast two-hybrid assay and identified an interaction between human HsYif1 and HsYip1A, a homolog of yeast Yip1. In addition, our immunoprecipitation pull-down assay validates the interaction between HsYif1 and HsYip1A. Moreover, our immunofluorescence study demonstrates the co-distribution of HsYif1 and HsYip1A. Significantly, over-expression of mutant HsYip1A-lacked cytosolic region disrupts the localization of HsYif1 to the Golgi, suggesting that HsYip1A specifies the localization of HsYif1 to the Golgi. Therefore, we conclude that human Yip1A interacts with and determines the localization of HsYif1 to the Golgi apparatus. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectSpecies Index: Saccharomycetalesen_US
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshEndoplasmic Reticulum - metabolismen_HK
dc.subject.meshGolgi Apparatus - metabolismen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney - metabolismen_HK
dc.subject.meshMembrane Proteins - chemistry - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshProtein Transport - physiologyen_HK
dc.subject.meshVesicular Transport Proteinsen_HK
dc.titleHuman Yip1A specifies the localization of Yif1 to the Golgi apparatusen_HK
dc.typeArticleen_HK
dc.identifier.emailFu, C:chuanhai@hku.hken_HK
dc.identifier.authorityFu, C=rp01515en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbrc.2005.06.051en_HK
dc.identifier.pmid15990086-
dc.identifier.scopuseid_2-s2.0-22144436316en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-22144436316&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume334en_HK
dc.identifier.issue1en_HK
dc.identifier.spage16en_HK
dc.identifier.epage22en_HK
dc.identifier.isiWOS:000230719400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJin, C=8583808800en_HK
dc.identifier.scopusauthoridZhang, Y=22954628200en_HK
dc.identifier.scopusauthoridZhu, H=8583808200en_HK
dc.identifier.scopusauthoridAhmed, K=7202086800en_HK
dc.identifier.scopusauthoridFu, C=8583808400en_HK
dc.identifier.scopusauthoridYao, X=7402530401en_HK

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