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Article: Crosslinkers and Motors Organize Dynamic Microtubules to Form Stable Bipolar Arrays in Fission Yeast

TitleCrosslinkers and Motors Organize Dynamic Microtubules to Form Stable Bipolar Arrays in Fission Yeast
Authors
KeywordsSpecies Index: Schizosaccharomycetaceae
Issue Date2007
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 2007, v. 128 n. 2, p. 357-368 How to Cite?
AbstractMicrotubule (MT) nucleation not only occurs from centrosomes, but also in large part from dispersed nucleation sites. The subsequent sorting of short MTs into networks like the mitotic spindle requires molecular motors that laterally slide overlapping MTs and bundling proteins that statically connect MTs. How bundling proteins interfere with MT sliding is unclear. In bipolar MT bundles in fission yeast, we found that the bundler ase1p localized all along the length of antiparallel MTs, whereas the motor klp2p (kinesin-14) accumulated only at MT plus ends. Consequently, sliding forces could only overcome resistant bundling forces for short, newly nucleated MTs, which were transported to their correct position within bundles. Ase1p thus regulated sliding forces based on polarity and overlap length, and computer simulations showed these mechanisms to be sufficient to generate stable bipolar bundles. By combining motor and bundling proteins, cells can thus dynamically organize stable regions of overlap between cytoskeletal filaments. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/136777
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJanson, MEen_HK
dc.contributor.authorLoughlin, Ren_HK
dc.contributor.authorLoïodice, Ien_HK
dc.contributor.authorFu, Cen_HK
dc.contributor.authorBrunner, Den_HK
dc.contributor.authorNédélec, FJen_HK
dc.contributor.authorTran, PTen_HK
dc.date.accessioned2011-07-29T02:12:03Z-
dc.date.available2011-07-29T02:12:03Z-
dc.date.issued2007en_HK
dc.identifier.citationCell, 2007, v. 128 n. 2, p. 357-368en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136777-
dc.description.abstractMicrotubule (MT) nucleation not only occurs from centrosomes, but also in large part from dispersed nucleation sites. The subsequent sorting of short MTs into networks like the mitotic spindle requires molecular motors that laterally slide overlapping MTs and bundling proteins that statically connect MTs. How bundling proteins interfere with MT sliding is unclear. In bipolar MT bundles in fission yeast, we found that the bundler ase1p localized all along the length of antiparallel MTs, whereas the motor klp2p (kinesin-14) accumulated only at MT plus ends. Consequently, sliding forces could only overcome resistant bundling forces for short, newly nucleated MTs, which were transported to their correct position within bundles. Ase1p thus regulated sliding forces based on polarity and overlap length, and computer simulations showed these mechanisms to be sufficient to generate stable bipolar bundles. By combining motor and bundling proteins, cells can thus dynamically organize stable regions of overlap between cytoskeletal filaments. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.subjectSpecies Index: Schizosaccharomycetaceaeen_US
dc.subject.meshActin Cytoskeleton - genetics - metabolism - ultrastructureen_HK
dc.subject.meshBinding Sitesen_HK
dc.subject.meshCarrier Proteins - genetics - metabolismen_HK
dc.subject.meshCell Polarity - physiologyen_HK
dc.subject.meshComputer Simulationen_HK
dc.subject.meshCytoplasmic Streaming - physiologyen_HK
dc.subject.meshCytoskeleton - genetics - metabolism - ultrastructureen_HK
dc.subject.meshMicrotubule-Associated Proteins - genetics - metabolismen_HK
dc.subject.meshMicrotubules - genetics - metabolism - ultrastructureen_HK
dc.subject.meshMitosis - physiologyen_HK
dc.subject.meshMitotic Spindle Apparatus - genetics - metabolism - ultrastructureen_HK
dc.subject.meshMolecular Motor Proteins - genetics - metabolismen_HK
dc.subject.meshSchizosaccharomyces - genetics - metabolism - ultrastructureen_HK
dc.subject.meshSchizosaccharomyces pombe Proteins - genetics - metabolismen_HK
dc.subject.meshStress, Mechanicalen_HK
dc.titleCrosslinkers and Motors Organize Dynamic Microtubules to Form Stable Bipolar Arrays in Fission Yeasten_HK
dc.typeArticleen_HK
dc.identifier.emailFu, C:chuanhai@hku.hken_HK
dc.identifier.authorityFu, C=rp01515en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.cell.2006.12.030en_HK
dc.identifier.pmid17254972en_HK
dc.identifier.scopuseid_2-s2.0-33846280179en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846280179&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume128en_HK
dc.identifier.issue2en_HK
dc.identifier.spage357en_HK
dc.identifier.epage368en_HK
dc.identifier.isiWOS:000244420500019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001064793-
dc.identifier.scopusauthoridJanson, ME=7004582356en_HK
dc.identifier.scopusauthoridLoughlin, R=24484686600en_HK
dc.identifier.scopusauthoridLoïodice, I=8259036100en_HK
dc.identifier.scopusauthoridFu, C=8583808400en_HK
dc.identifier.scopusauthoridBrunner, D=7102808008en_HK
dc.identifier.scopusauthoridNédélec, FJ=6602974656en_HK
dc.identifier.scopusauthoridTran, PT=7102073156en_HK
dc.identifier.citeulike1375672-

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