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Article: Fast microfluidic temperature control for high resolution live cell imaging

TitleFast microfluidic temperature control for high resolution live cell imaging
Authors
KeywordsSpecies Index: Schizosaccharomyces Pombe
Schizosaccharomycetaceae
Issue Date2011
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc
Citation
Lab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 3, p. 484-489 How to Cite?
AbstractOne major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle. © 2011 The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/136772
ISSN
2015 Impact Factor: 5.586
2015 SCImago Journal Rankings: 2.373
ISI Accession Number ID
Funding AgencyGrant Number
JCS
ARC
NIH
ACS
HFPS
FRM
ANR
MarieParis
LaLigue
Funding Information:

We thank our respective lab members for helpful comments and suggestions. GVC, JC and FC performed experiments. GVC and PTT wrote the paper. All others contributed technological insights and equipment. GVC is supported by a traveling fellowship from JCS and a postdoctoral fellowship from ARC.

References

 

DC FieldValueLanguage
dc.contributor.authorVelve Casquillas, Gen_HK
dc.contributor.authorFu, Cen_HK
dc.contributor.authorLe Berre, Men_HK
dc.contributor.authorCramer, Jen_HK
dc.contributor.authorMeance, Sen_HK
dc.contributor.authorPlecis, Aen_HK
dc.contributor.authorBaigl, Den_HK
dc.contributor.authorGreffet, JJen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorPiel, Men_HK
dc.contributor.authorTran, PTen_HK
dc.date.accessioned2011-07-29T02:11:51Z-
dc.date.available2011-07-29T02:11:51Z-
dc.date.issued2011en_HK
dc.identifier.citationLab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 3, p. 484-489en_HK
dc.identifier.issn1473-0197en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136772-
dc.description.abstractOne major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle. © 2011 The Royal Society of Chemistry.en_HK
dc.languageengen_US
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/locen_HK
dc.relation.ispartofLab on a Chip - Miniaturisation for Chemistry and Biologyen_HK
dc.subjectSpecies Index: Schizosaccharomyces Pombeen_US
dc.subjectSchizosaccharomycetaceaeen_US
dc.subject.meshCell Cycleen_HK
dc.subject.meshDimethylpolysiloxanes - chemistryen_HK
dc.subject.meshGreen Fluorescent Proteins - analysisen_HK
dc.subject.meshMicrofluidic Analytical Techniques - instrumentation - methodsen_HK
dc.subject.meshMicrotechnology - methodsen_HK
dc.subject.meshMicrotubulesen_HK
dc.subject.meshNylons - chemistryen_HK
dc.subject.meshSchizosaccharomyces - cytologyen_HK
dc.subject.meshTemperatureen_HK
dc.titleFast microfluidic temperature control for high resolution live cell imagingen_HK
dc.typeArticleen_HK
dc.identifier.emailFu, C:chuanhai@hku.hken_HK
dc.identifier.authorityFu, C=rp01515en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1039/c0lc00222den_HK
dc.identifier.pmid21103458-
dc.identifier.scopuseid_2-s2.0-78751539941en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78751539941&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue3en_HK
dc.identifier.spage484en_HK
dc.identifier.epage489en_HK
dc.identifier.eissn1473-0189-
dc.identifier.isiWOS:000286326700019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridVelve Casquillas, G=13008971800en_HK
dc.identifier.scopusauthoridFu, C=8583808400en_HK
dc.identifier.scopusauthoridLe Berre, M=8257613700en_HK
dc.identifier.scopusauthoridCramer, J=37664596000en_HK
dc.identifier.scopusauthoridMeance, S=23485965400en_HK
dc.identifier.scopusauthoridPlecis, A=16245911500en_HK
dc.identifier.scopusauthoridBaigl, D=35247409800en_HK
dc.identifier.scopusauthoridGreffet, JJ=35310080200en_HK
dc.identifier.scopusauthoridChen, Y=35309684800en_HK
dc.identifier.scopusauthoridPiel, M=6701852773en_HK
dc.identifier.scopusauthoridTran, PT=7102073156en_HK

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