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- Publisher Website: 10.1039/c0lc00222d
- Scopus: eid_2-s2.0-78751539941
- PMID: 21103458
- WOS: WOS:000286326700019
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Article: Fast microfluidic temperature control for high resolution live cell imaging
Title | Fast microfluidic temperature control for high resolution live cell imaging | ||||||||||||||||||||
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Authors | |||||||||||||||||||||
Keywords | Species Index: Schizosaccharomyces Pombe Schizosaccharomycetaceae | ||||||||||||||||||||
Issue Date | 2011 | ||||||||||||||||||||
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc | ||||||||||||||||||||
Citation | Lab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 3, p. 484-489 How to Cite? | ||||||||||||||||||||
Abstract | One major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle. © 2011 The Royal Society of Chemistry. | ||||||||||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/136772 | ||||||||||||||||||||
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.246 | ||||||||||||||||||||
ISI Accession Number ID |
Funding Information: We thank our respective lab members for helpful comments and suggestions. GVC, JC and FC performed experiments. GVC and PTT wrote the paper. All others contributed technological insights and equipment. GVC is supported by a traveling fellowship from JCS and a postdoctoral fellowship from ARC. | ||||||||||||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Velve Casquillas, G | en_HK |
dc.contributor.author | Fu, C | en_HK |
dc.contributor.author | Le Berre, M | en_HK |
dc.contributor.author | Cramer, J | en_HK |
dc.contributor.author | Meance, S | en_HK |
dc.contributor.author | Plecis, A | en_HK |
dc.contributor.author | Baigl, D | en_HK |
dc.contributor.author | Greffet, JJ | en_HK |
dc.contributor.author | Chen, Y | en_HK |
dc.contributor.author | Piel, M | en_HK |
dc.contributor.author | Tran, PT | en_HK |
dc.date.accessioned | 2011-07-29T02:11:51Z | - |
dc.date.available | 2011-07-29T02:11:51Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Lab On A Chip - Miniaturisation For Chemistry And Biology, 2011, v. 11 n. 3, p. 484-489 | en_HK |
dc.identifier.issn | 1473-0197 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/136772 | - |
dc.description.abstract | One major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle. © 2011 The Royal Society of Chemistry. | en_HK |
dc.language | eng | en_US |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/loc | en_HK |
dc.relation.ispartof | Lab on a Chip - Miniaturisation for Chemistry and Biology | en_HK |
dc.subject | Species Index: Schizosaccharomyces Pombe | en_US |
dc.subject | Schizosaccharomycetaceae | en_US |
dc.subject.mesh | Cell Cycle | en_HK |
dc.subject.mesh | Dimethylpolysiloxanes - chemistry | en_HK |
dc.subject.mesh | Green Fluorescent Proteins - analysis | en_HK |
dc.subject.mesh | Microfluidic Analytical Techniques - instrumentation - methods | en_HK |
dc.subject.mesh | Microtechnology - methods | en_HK |
dc.subject.mesh | Microtubules | en_HK |
dc.subject.mesh | Nylons - chemistry | en_HK |
dc.subject.mesh | Schizosaccharomyces - cytology | en_HK |
dc.subject.mesh | Temperature | en_HK |
dc.title | Fast microfluidic temperature control for high resolution live cell imaging | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Fu, C:chuanhai@hku.hk | en_HK |
dc.identifier.authority | Fu, C=rp01515 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1039/c0lc00222d | en_HK |
dc.identifier.pmid | 21103458 | - |
dc.identifier.scopus | eid_2-s2.0-78751539941 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-78751539941&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 11 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 484 | en_HK |
dc.identifier.epage | 489 | en_HK |
dc.identifier.eissn | 1473-0189 | - |
dc.identifier.isi | WOS:000286326700019 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Velve Casquillas, G=13008971800 | en_HK |
dc.identifier.scopusauthorid | Fu, C=8583808400 | en_HK |
dc.identifier.scopusauthorid | Le Berre, M=8257613700 | en_HK |
dc.identifier.scopusauthorid | Cramer, J=37664596000 | en_HK |
dc.identifier.scopusauthorid | Meance, S=23485965400 | en_HK |
dc.identifier.scopusauthorid | Plecis, A=16245911500 | en_HK |
dc.identifier.scopusauthorid | Baigl, D=35247409800 | en_HK |
dc.identifier.scopusauthorid | Greffet, JJ=35310080200 | en_HK |
dc.identifier.scopusauthorid | Chen, Y=35309684800 | en_HK |
dc.identifier.scopusauthorid | Piel, M=6701852773 | en_HK |
dc.identifier.scopusauthorid | Tran, PT=7102073156 | en_HK |
dc.identifier.issnl | 1473-0189 | - |