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Conference Paper: Anti-metastatic mechanism of Tian-Xian Liquid (TXL) and its bioactive fractions in human colorectal cancer cells and xenograft models

TitleAnti-metastatic mechanism of Tian-Xian Liquid (TXL) and its bioactive fractions in human colorectal cancer cells and xenograft models
Authors
Issue Date2010
PublisherNature - Centro Nacional de Investigaciones Oncologicas (CNIO).
Citation
The 2010 Nature CNIO Cancer Symposium on Frontiers in Tumour Progression, Madrid, Spain, 24-27 October 2010. In Frontiers in Tumour Progression, 2010 , p. 116, abstract no. 29 How to Cite?
AbstractColorectal carcinoma is the second most prevalent cancer with an up-rising trend in Hong Kong (Hong Kong Cancer Registry). Traditional Chinese medicine acts as a complementary alternative for tumour therapy with minimal side-effects and traumatic injuries. Tian-Xian Liquid (TXL), one of the well-known natural medicinal herbal formulations, has been commercially used as an anticancer dietary supplement for a decade without known adverse effects. This study aimed to investigate the anti-metastatic property of TXL and its bioactive fractions [butanol fraction (BU), ethyl-acetate fraction (EA) and aqueous fraction (WA)] at molecular level on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mice xenografts). For the cell model, TXL and its bioactive fractions have similar anti-proliferative effects by MTT assay. At 4-hour-incubation, IC50 values were obtained at 1% (V/V) TXL, 1.25% (V/V) BU, 5% (V/V) EA and 0.3125% (V/V) WA. At IC50, TXL and its bioactive fractions significantly reduced the MMP2 and MMP7 expressions at mRNA level by real-time PCR. At protein level, TXL, BU and EA correspondingly down-regulated MMP2 (active form) and MMP7 protein from 24 to 48 hours; TXL and BU also down-regulated VEGF protein expression; however, no such effect was found in WA-treated cells. Further, only TXL, EA and WA effectively inhibited the cell migration at 48 hours incubation by woundhealing assay. For the xenografts models, MMP2 and MMP7 mRNA expressions were reduced by TXL-, BU- and EA-treated xenografts; however no effects on MMP2 protein expression in all drug-treated xenografts. The VEGF protein expression was significantly down-regulated in TXL- and WA-treated xenografts. Further, TXL, BU and WA effectively inhibited the tumor growth without altering the body weight of the xenografts. In summary, the Chinese medicinal formulation, TXL, demonstrated the most effective anti-metastatic ability on human colorectal cancer in vitro and in vivo.
DescriptionPoster Session A: abstract no. 29
Persistent Identifierhttp://hdl.handle.net/10722/136393

 

DC FieldValueLanguage
dc.contributor.authorChu, ESMen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorCheung, HPen_US
dc.contributor.authorLiu, WKen_US
dc.contributor.authorNg, TBen_US
dc.contributor.authorTong, Y-
dc.date.accessioned2011-07-27T02:14:55Z-
dc.date.available2011-07-27T02:14:55Z-
dc.date.issued2010en_US
dc.identifier.citationThe 2010 Nature CNIO Cancer Symposium on Frontiers in Tumour Progression, Madrid, Spain, 24-27 October 2010. In Frontiers in Tumour Progression, 2010 , p. 116, abstract no. 29en_US
dc.identifier.urihttp://hdl.handle.net/10722/136393-
dc.descriptionPoster Session A: abstract no. 29-
dc.description.abstractColorectal carcinoma is the second most prevalent cancer with an up-rising trend in Hong Kong (Hong Kong Cancer Registry). Traditional Chinese medicine acts as a complementary alternative for tumour therapy with minimal side-effects and traumatic injuries. Tian-Xian Liquid (TXL), one of the well-known natural medicinal herbal formulations, has been commercially used as an anticancer dietary supplement for a decade without known adverse effects. This study aimed to investigate the anti-metastatic property of TXL and its bioactive fractions [butanol fraction (BU), ethyl-acetate fraction (EA) and aqueous fraction (WA)] at molecular level on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mice xenografts). For the cell model, TXL and its bioactive fractions have similar anti-proliferative effects by MTT assay. At 4-hour-incubation, IC50 values were obtained at 1% (V/V) TXL, 1.25% (V/V) BU, 5% (V/V) EA and 0.3125% (V/V) WA. At IC50, TXL and its bioactive fractions significantly reduced the MMP2 and MMP7 expressions at mRNA level by real-time PCR. At protein level, TXL, BU and EA correspondingly down-regulated MMP2 (active form) and MMP7 protein from 24 to 48 hours; TXL and BU also down-regulated VEGF protein expression; however, no such effect was found in WA-treated cells. Further, only TXL, EA and WA effectively inhibited the cell migration at 48 hours incubation by woundhealing assay. For the xenografts models, MMP2 and MMP7 mRNA expressions were reduced by TXL-, BU- and EA-treated xenografts; however no effects on MMP2 protein expression in all drug-treated xenografts. The VEGF protein expression was significantly down-regulated in TXL- and WA-treated xenografts. Further, TXL, BU and WA effectively inhibited the tumor growth without altering the body weight of the xenografts. In summary, the Chinese medicinal formulation, TXL, demonstrated the most effective anti-metastatic ability on human colorectal cancer in vitro and in vivo.-
dc.languageengen_US
dc.publisherNature - Centro Nacional de Investigaciones Oncologicas (CNIO).-
dc.relation.ispartofFrontiers in Tumour Progression 2010: programme & abstractsen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleAnti-metastatic mechanism of Tian-Xian Liquid (TXL) and its bioactive fractions in human colorectal cancer cells and xenograft modelsen_US
dc.typeConference_Paperen_US
dc.identifier.emailChu, ESM: elliecsm@hku.hken_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.emailCheung, HP: bunnub@hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.description.naturepublished_or_final_version-
dc.identifier.hkuros188148en_US
dc.identifier.spage116en_US
dc.identifier.epage116en_US
dc.publisher.placeSpain-

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