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Article: Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis

TitleMorphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis
Authors
Issue Date2009
PublisherSpringer Verlag.
Citation
Journal Of Industrial Microbiology And Biotechnology, 2009, v. 36 n. 6, p. 787-800 How to Cite?
AbstractMycelial morphology is a critically important process property in industrial fermentations of filamentous micro-organisms, as particular phenotypes are associated with maximum productivity. However, the accurate quantification of complex morphologies still represents a significant challenge in elucidating this relationship. A system has been developed for high-resolution characterisation of filamentous fungal growth on a solid substrate, using membrane immobilization and fully-automatic plug-ins developed for the public domain, Java-based, image-processing software, ImageJ. The system has been used to quantify the microscopic development of Aspergillus oryzae on malt agar, by measuring spore projected area and circularity, the total length of a hyphal element, the number of tips per element, and the hyphal growth unit. Two different stages of growth are described, from the swelling of a population of conidiospores up to fully developed, branched hyphae 24 h after inoculation. Spore swelling expressed as an increase in mean equivalent spore diameter was found to be approximately linear with time. Widespread germination of spores was observed by 8 h after inoculation. From approximately 12 h, the number of tips was found to increase exponentially. The specific growth rate of a population of hyphae was calculated as approximately 0.24-0.27 h -1. A wide variation in growth kinetics was found within the population. The robustness of the image-analysis system was verified by testing the effect of small variations in the input data. © 2009 Society for Industrial Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/136354
ISSN
2015 Impact Factor: 2.745
2015 SCImago Journal Rankings: 0.966
ISI Accession Number ID
Funding AgencyGrant Number
Technological Sector Research, Strand 1 Postgraduate R&D Skills Programme
Funding Information:

This work has been supported by a grant from the Technological Sector Research, Strand 1 Postgraduate R&D Skills Programme. We thank P. Taylor for her technical assistance in this study.

 

DC FieldValueLanguage
dc.contributor.authorBarry, DJen_HK
dc.contributor.authorChan, Cen_HK
dc.contributor.authorWilliams, GAen_HK
dc.date.accessioned2011-07-27T02:14:14Z-
dc.date.available2011-07-27T02:14:14Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Industrial Microbiology And Biotechnology, 2009, v. 36 n. 6, p. 787-800en_HK
dc.identifier.issn1367-5435en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136354-
dc.description.abstractMycelial morphology is a critically important process property in industrial fermentations of filamentous micro-organisms, as particular phenotypes are associated with maximum productivity. However, the accurate quantification of complex morphologies still represents a significant challenge in elucidating this relationship. A system has been developed for high-resolution characterisation of filamentous fungal growth on a solid substrate, using membrane immobilization and fully-automatic plug-ins developed for the public domain, Java-based, image-processing software, ImageJ. The system has been used to quantify the microscopic development of Aspergillus oryzae on malt agar, by measuring spore projected area and circularity, the total length of a hyphal element, the number of tips per element, and the hyphal growth unit. Two different stages of growth are described, from the swelling of a population of conidiospores up to fully developed, branched hyphae 24 h after inoculation. Spore swelling expressed as an increase in mean equivalent spore diameter was found to be approximately linear with time. Widespread germination of spores was observed by 8 h after inoculation. From approximately 12 h, the number of tips was found to increase exponentially. The specific growth rate of a population of hyphae was calculated as approximately 0.24-0.27 h -1. A wide variation in growth kinetics was found within the population. The robustness of the image-analysis system was verified by testing the effect of small variations in the input data. © 2009 Society for Industrial Microbiology.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag.en_US
dc.relation.ispartofJournal of Industrial Microbiology and Biotechnologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshAspergillus oryzae - chemistry - growth and development-
dc.subject.meshCulture Techniques-
dc.subject.meshImage Processing, Computer-Assisted-
dc.subject.meshMembranes, Artificial-
dc.subject.meshSpores, Fungal - chemistry - growth and development-
dc.titleMorphological quantification of filamentous fungal development using membrane immobilization and automatic image analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1367-5435&volume=36&issue=6&spage=787&epage=800&date=2009&atitle=Morphological+quantification+of+filamentous+fungal+development+using+membrane+immobilization+and+automatic+image+analysisen_US
dc.identifier.emailChan, C:cecilia.chan@caut.hku.hken_HK
dc.identifier.authorityChan, C=rp00892en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10295-009-0552-9en_HK
dc.identifier.pmid19277741-
dc.identifier.scopuseid_2-s2.0-67649103626en_HK
dc.identifier.hkuros158729en_US
dc.identifier.volume36en_HK
dc.identifier.issue6en_HK
dc.identifier.spage787en_HK
dc.identifier.epage800en_HK
dc.identifier.isiWOS:000266385900002-
dc.publisher.placeGermanyen_HK
dc.identifier.citeulike4176991-

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