Article: Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury
| Title | Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Li, SY2 Yang, D2 Yeung, CM2 Yu, WY2 Chang, RCC2 So, KF2 Wong, D1 2 Lo, ACY2 | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| Citation | Plos One, 2011, v. 6 n. 1 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0016380 | ||||||
| Abstract | Neuronal cell death, glial cell activation, retinal swelling and oxidative injury are complications in retinal ischemia/ reperfusion (I/R) injuries. Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are good for "eye health" according to Chinese medicine. The aim of our present study is to explore the use of LBP in retinal I/R injury. Retinal I/R injury was induced by surgical occlusion of the internal carotid artery. Prior to induction of ischemia, mice were treated orally with either vehicle (PBS) or LBP (1 mg/kg) once a day for 1 week. Paraffin-embedded retinal sections were prepared. Viable cells were counted; apoptosis was assessed using TUNEL assay. Expression levels of glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), poly(ADP-ribose) (PAR) and nitrotyrosine (NT) were investigated by immunohistochemistry. The integrity of blood-retinal barrier (BRB) was examined by IgG extravasations. Apoptosis and decreased viable cell count were found in the ganglion cell layer (GCL) and the inner nuclear layer (INL) of the vehicle-treated I/R retina. Additionally, increased retinal thickness, GFAP activation, AQP4 up-regulation, IgG extravasations and PAR expression levels were observed in the vehicle-treated I/R retina. Many of these changes were diminished or abolished in the LBP-treated I/R retina. Pre-treatment with LBP for 1 week effectively protected the retina from neuronal death, apoptosis, glial cell activation, aquaporin water channel up-regulation, disruption of BRB and oxidative stress. The present study suggests that LBP may have a neuroprotective role to play in ocular diseases for which I/R is a feature. © 2011 Li et al. | ||||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0016380 | ||||||
| ISI Accession Number ID | WOS:000286662800034
Funding Information: This project was supported by the Small Project Funding (C.M. Yeung) and the University Development Fund from The University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| PubMed Central ID | PMC3027646 | ||||||
| References | References in Scopus |
| dc.contributor.author | Li, SY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Yang, D | ||||||
| dc.contributor.author | Yeung, CM | ||||||
| dc.contributor.author | Yu, WY | ||||||
| dc.contributor.author | Chang, RCC | ||||||
| dc.contributor.author | So, KF | ||||||
| dc.contributor.author | Wong, D | ||||||
| dc.contributor.author | Lo, ACY | ||||||
| dc.date.accessioned | 2011-07-27T02:13:36Z | ||||||
| dc.date.available | 2011-07-27T02:13:36Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Neuronal cell death, glial cell activation, retinal swelling and oxidative injury are complications in retinal ischemia/ reperfusion (I/R) injuries. Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are good for "eye health" according to Chinese medicine. The aim of our present study is to explore the use of LBP in retinal I/R injury. Retinal I/R injury was induced by surgical occlusion of the internal carotid artery. Prior to induction of ischemia, mice were treated orally with either vehicle (PBS) or LBP (1 mg/kg) once a day for 1 week. Paraffin-embedded retinal sections were prepared. Viable cells were counted; apoptosis was assessed using TUNEL assay. Expression levels of glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), poly(ADP-ribose) (PAR) and nitrotyrosine (NT) were investigated by immunohistochemistry. The integrity of blood-retinal barrier (BRB) was examined by IgG extravasations. Apoptosis and decreased viable cell count were found in the ganglion cell layer (GCL) and the inner nuclear layer (INL) of the vehicle-treated I/R retina. Additionally, increased retinal thickness, GFAP activation, AQP4 up-regulation, IgG extravasations and PAR expression levels were observed in the vehicle-treated I/R retina. Many of these changes were diminished or abolished in the LBP-treated I/R retina. Pre-treatment with LBP for 1 week effectively protected the retina from neuronal death, apoptosis, glial cell activation, aquaporin water channel up-regulation, disruption of BRB and oxidative stress. The present study suggests that LBP may have a neuroprotective role to play in ocular diseases for which I/R is a feature. © 2011 Li et al. | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.identifier.citation | Plos One, 2011, v. 6 n. 1 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0016380 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0016380 | ||||||
| dc.identifier.epage | e16380 | ||||||
| dc.identifier.hkuros | 186642 | ||||||
| dc.identifier.isi | WOS:000286662800034
Funding Information: This project was supported by the Small Project Funding (C.M. Yeung) and the University Development Fund from The University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ||||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.pmcid | PMC3027646 | ||||||
| dc.identifier.pmid | 21298100 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79551517589 | ||||||
| dc.identifier.spage | e16380 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/136331 | ||||||
| dc.identifier.volume | 6 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | PLoS ONE | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||
| dc.subject.mesh | Blood-Retinal Barrier - drug effects - physiopathology | ||||||
| dc.subject.mesh | Drugs, Chinese Herbal - pharmacology - therapeutic use | ||||||
| dc.subject.mesh | Neurons - drug effects - pathology | ||||||
| dc.subject.mesh | Oxidative Stress - drug effects | ||||||
| dc.subject.mesh | Reperfusion Injury - drug therapy | ||||||
| dc.title | Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury | ||||||
| dc.type | Article |
Author Affiliations
- null
- The University of Hong Kong Li Ka Shing Faculty of Medicine