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Article: Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury
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TitleLycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury
 
AuthorsLi, SY1
Yang, D1
Yeung, CM1
Yu, WY1
Chang, RCC1
So, KF1
Wong, D1 2
Lo, ACY1
 
Issue Date2011
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPlos One, 2011, v. 6 n. 1, article no. e16380 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0016380
 
AbstractNeuronal cell death, glial cell activation, retinal swelling and oxidative injury are complications in retinal ischemia/ reperfusion (I/R) injuries. Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are good for "eye health" according to Chinese medicine. The aim of our present study is to explore the use of LBP in retinal I/R injury. Retinal I/R injury was induced by surgical occlusion of the internal carotid artery. Prior to induction of ischemia, mice were treated orally with either vehicle (PBS) or LBP (1 mg/kg) once a day for 1 week. Paraffin-embedded retinal sections were prepared. Viable cells were counted; apoptosis was assessed using TUNEL assay. Expression levels of glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), poly(ADP-ribose) (PAR) and nitrotyrosine (NT) were investigated by immunohistochemistry. The integrity of blood-retinal barrier (BRB) was examined by IgG extravasations. Apoptosis and decreased viable cell count were found in the ganglion cell layer (GCL) and the inner nuclear layer (INL) of the vehicle-treated I/R retina. Additionally, increased retinal thickness, GFAP activation, AQP4 up-regulation, IgG extravasations and PAR expression levels were observed in the vehicle-treated I/R retina. Many of these changes were diminished or abolished in the LBP-treated I/R retina. Pre-treatment with LBP for 1 week effectively protected the retina from neuronal death, apoptosis, glial cell activation, aquaporin water channel up-regulation, disruption of BRB and oxidative stress. The present study suggests that LBP may have a neuroprotective role to play in ocular diseases for which I/R is a feature. © 2011 Li et al.
 
ISSN1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0016380
 
PubMed Central IDPMC3027646
 
ISI Accession Number IDWOS:000286662800034
Funding AgencyGrant Number
Small Project Funding
The University of Hong Kong
Funding Information:

This project was supported by the Small Project Funding (C.M. Yeung) and the University Development Fund from The University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, SY
 
dc.contributor.authorYang, D
 
dc.contributor.authorYeung, CM
 
dc.contributor.authorYu, WY
 
dc.contributor.authorChang, RCC
 
dc.contributor.authorSo, KF
 
dc.contributor.authorWong, D
 
dc.contributor.authorLo, ACY
 
dc.date.accessioned2011-07-27T02:13:36Z
 
dc.date.available2011-07-27T02:13:36Z
 
dc.date.issued2011
 
dc.description.abstractNeuronal cell death, glial cell activation, retinal swelling and oxidative injury are complications in retinal ischemia/ reperfusion (I/R) injuries. Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are good for "eye health" according to Chinese medicine. The aim of our present study is to explore the use of LBP in retinal I/R injury. Retinal I/R injury was induced by surgical occlusion of the internal carotid artery. Prior to induction of ischemia, mice were treated orally with either vehicle (PBS) or LBP (1 mg/kg) once a day for 1 week. Paraffin-embedded retinal sections were prepared. Viable cells were counted; apoptosis was assessed using TUNEL assay. Expression levels of glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), poly(ADP-ribose) (PAR) and nitrotyrosine (NT) were investigated by immunohistochemistry. The integrity of blood-retinal barrier (BRB) was examined by IgG extravasations. Apoptosis and decreased viable cell count were found in the ganglion cell layer (GCL) and the inner nuclear layer (INL) of the vehicle-treated I/R retina. Additionally, increased retinal thickness, GFAP activation, AQP4 up-regulation, IgG extravasations and PAR expression levels were observed in the vehicle-treated I/R retina. Many of these changes were diminished or abolished in the LBP-treated I/R retina. Pre-treatment with LBP for 1 week effectively protected the retina from neuronal death, apoptosis, glial cell activation, aquaporin water channel up-regulation, disruption of BRB and oxidative stress. The present study suggests that LBP may have a neuroprotective role to play in ocular diseases for which I/R is a feature. © 2011 Li et al.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPlos One, 2011, v. 6 n. 1, article no. e16380 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0016380
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0016380
 
dc.identifier.eissn1932-6203
 
dc.identifier.epagee16380
 
dc.identifier.epagearticle no. e16380
 
dc.identifier.hkuros186642
 
dc.identifier.isiWOS:000286662800034
Funding AgencyGrant Number
Small Project Funding
The University of Hong Kong
Funding Information:

This project was supported by the Small Project Funding (C.M. Yeung) and the University Development Fund from The University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
dc.identifier.issue1
 
dc.identifier.pmcidPMC3027646
 
dc.identifier.pmid21298100
 
dc.identifier.scopuseid_2-s2.0-79551517589
 
dc.identifier.spagee16380
 
dc.identifier.spagearticle no. e16380
 
dc.identifier.urihttp://hdl.handle.net/10722/136331
 
dc.identifier.volume6
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS ONE
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshBlood-Retinal Barrier - drug effects - physiopathology
 
dc.subject.meshDrugs, Chinese Herbal - pharmacology - therapeutic use
 
dc.subject.meshNeurons - drug effects - pathology
 
dc.subject.meshOxidative Stress - drug effects
 
dc.subject.meshReperfusion Injury - drug therapy
 
dc.titleLycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. null