Article: Differential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer models
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- Publisher Website: 10.1016/j.jep.2011.05.035
- Scopus: eid_2-s2.0-80052035168
- PMID: 21669277
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| Title | Differential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer models | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Chu, ESM1 Sze, SCW1 Cheung, HP1 Wong, KL1 Liu, Q1 Ng, TB2 Tong, Y1 | ||||||
| Keywords | Anti-metastatic Chinese medicinal formulation Colorectal cancer Matrix metalloproteinases Tian-Xian liquid Vascular endothelial growth factor | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm | ||||||
| Citation | Journal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 403-413 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jep.2011.05.035 | ||||||
| Abstract | Aim of study: This study aimed to elucidate and compare the anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive components namely butanol (BU), ethyl-acetate (EA) and aqueous (WA) fractions on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mouse xenografts). Materials and methods: The anti-proliferative effects of TXL and its bioactive components in HT-29 cells were determined by MTT assay. Their modulations on the potential angiogenic and metastatic marker expressions on HT-29 cells and xenografts were investigated by real-time PCR and Western blot at transcriptional and translational levels, respectively. For the in vitro study, migration abilities of HT-29 cells were determined using wound healing assay. For the in vivo study, daily measurements of the tumor size and volume of the xenografts were also performed. Results: TXL, BU, EA and WA effectively inhibited the proliferation of HT-29 cells in a dose- and time-dependent manner. The IC 50 value of TXL on HT-29 cells was obtained after incubation with 1% (v/v) TXL for 4 h; whereas IC 50 values were obtained for the following bioactive components: BU at 1.25% (v/v); EA at 5% (v/v); and WA at 0.3125% (v/v). It was found that 1% (v/v) TXL significantly down-regulated MMP2 and MMP7 expression at both transcriptional and translational levels and it reduced MMP9 and VEGF protein expression in vitro. TXL decreased the metastatic ability of HT-29 cells as demonstrated by wound healing assay. TXL and its bioactive fractions caused no significant changes in the body weight indicating lack of toxicity to the xenografts. Conclusions: In summary, TXL multi-targeted to down-regulate the metastatic markers in both in vitro and in vivo models. However, the effects of its bioactive fractions were not obvious. This study profoundly elucidated the anti-proliferative mechanism of TXL, which is vital for the development of future anti-cancer regime in Chinese medicinal formulations. © 2011 Elsevier Ireland Ltd All rights reserved. | ||||||
| ISSN | 0378-8741 2011 Impact Factor: 3.014 2011 SCImago Journal Rankings: 0.114 | ||||||
| DOI | http://dx.doi.org/10.1016/j.jep.2011.05.035 | ||||||
| ISI Accession Number ID | WOS:000295236700050
Funding Information: The research study was supported in part by a grant from Seed Funding Programme for Applied Research (no.: 200807160015) and contract research funding from China-Japan Feida Union Company Limited. | ||||||
| References | References in Scopus | ||||||
| Grants | Bioactive components of Tian Xian Liquid as a potential drug candidate for the treatment of colon cancer |
| dc.contributor.author | Chu, ESM | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Sze, SCW | ||||||
| dc.contributor.author | Cheung, HP | ||||||
| dc.contributor.author | Wong, KL | ||||||
| dc.contributor.author | Liu, Q | ||||||
| dc.contributor.author | Ng, TB | ||||||
| dc.contributor.author | Tong, Y | ||||||
| dc.date.accessioned | 2011-07-27T02:12:56Z | ||||||
| dc.date.available | 2011-07-27T02:12:56Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Aim of study: This study aimed to elucidate and compare the anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive components namely butanol (BU), ethyl-acetate (EA) and aqueous (WA) fractions on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mouse xenografts). Materials and methods: The anti-proliferative effects of TXL and its bioactive components in HT-29 cells were determined by MTT assay. Their modulations on the potential angiogenic and metastatic marker expressions on HT-29 cells and xenografts were investigated by real-time PCR and Western blot at transcriptional and translational levels, respectively. For the in vitro study, migration abilities of HT-29 cells were determined using wound healing assay. For the in vivo study, daily measurements of the tumor size and volume of the xenografts were also performed. Results: TXL, BU, EA and WA effectively inhibited the proliferation of HT-29 cells in a dose- and time-dependent manner. The IC 50 value of TXL on HT-29 cells was obtained after incubation with 1% (v/v) TXL for 4 h; whereas IC 50 values were obtained for the following bioactive components: BU at 1.25% (v/v); EA at 5% (v/v); and WA at 0.3125% (v/v). It was found that 1% (v/v) TXL significantly down-regulated MMP2 and MMP7 expression at both transcriptional and translational levels and it reduced MMP9 and VEGF protein expression in vitro. TXL decreased the metastatic ability of HT-29 cells as demonstrated by wound healing assay. TXL and its bioactive fractions caused no significant changes in the body weight indicating lack of toxicity to the xenografts. Conclusions: In summary, TXL multi-targeted to down-regulate the metastatic markers in both in vitro and in vivo models. However, the effects of its bioactive fractions were not obvious. This study profoundly elucidated the anti-proliferative mechanism of TXL, which is vital for the development of future anti-cancer regime in Chinese medicinal formulations. © 2011 Elsevier Ireland Ltd All rights reserved. | ||||||
| dc.description.grant | Bioactive components of Tian Xian Liquid as a potential drug candidate for the treatment of colon cancer | ||||||
| dc.description.grantcode | 99392 | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 403-413 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jep.2011.05.035 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.jep.2011.05.035 | ||||||
| dc.identifier.epage | 413 | ||||||
| dc.identifier.hkuros | 188145 | ||||||
| dc.identifier.isi | WOS:000295236700050
Funding Information: The research study was supported in part by a grant from Seed Funding Programme for Applied Research (no.: 200807160015) and contract research funding from China-Japan Feida Union Company Limited. | ||||||
| dc.identifier.issn | 0378-8741 2011 Impact Factor: 3.014 2011 SCImago Journal Rankings: 0.114 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 21669277 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80052035168 | ||||||
| dc.identifier.spage | 403 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/136308 | ||||||
| dc.identifier.volume | 137 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm | ||||||
| dc.publisher.place | Ireland | ||||||
| dc.relation.ispartof | Journal of Ethnopharmacology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Anti-metastatic | ||||||
| dc.subject | Chinese medicinal formulation | ||||||
| dc.subject | Colorectal cancer | ||||||
| dc.subject | Matrix metalloproteinases | ||||||
| dc.subject | Tian-Xian liquid | ||||||
| dc.subject | Vascular endothelial growth factor | ||||||
| dc.title | Differential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer models | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- Chinese University of Hong Kong