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Article: Differential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer models

TitleDifferential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer models
Authors
KeywordsAnti-metastatic
Chinese medicinal formulation
Colorectal cancer
Matrix metalloproteinases
Tian-Xian liquid
Vascular endothelial growth factor
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 403-413 How to Cite?
Abstract
Aim of study: This study aimed to elucidate and compare the anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive components namely butanol (BU), ethyl-acetate (EA) and aqueous (WA) fractions on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mouse xenografts). Materials and methods: The anti-proliferative effects of TXL and its bioactive components in HT-29 cells were determined by MTT assay. Their modulations on the potential angiogenic and metastatic marker expressions on HT-29 cells and xenografts were investigated by real-time PCR and Western blot at transcriptional and translational levels, respectively. For the in vitro study, migration abilities of HT-29 cells were determined using wound healing assay. For the in vivo study, daily measurements of the tumor size and volume of the xenografts were also performed. Results: TXL, BU, EA and WA effectively inhibited the proliferation of HT-29 cells in a dose- and time-dependent manner. The IC 50 value of TXL on HT-29 cells was obtained after incubation with 1% (v/v) TXL for 4 h; whereas IC 50 values were obtained for the following bioactive components: BU at 1.25% (v/v); EA at 5% (v/v); and WA at 0.3125% (v/v). It was found that 1% (v/v) TXL significantly down-regulated MMP2 and MMP7 expression at both transcriptional and translational levels and it reduced MMP9 and VEGF protein expression in vitro. TXL decreased the metastatic ability of HT-29 cells as demonstrated by wound healing assay. TXL and its bioactive fractions caused no significant changes in the body weight indicating lack of toxicity to the xenografts. Conclusions: In summary, TXL multi-targeted to down-regulate the metastatic markers in both in vitro and in vivo models. However, the effects of its bioactive fractions were not obvious. This study profoundly elucidated the anti-proliferative mechanism of TXL, which is vital for the development of future anti-cancer regime in Chinese medicinal formulations. © 2011 Elsevier Ireland Ltd All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/136308
ISSN
2013 Impact Factor: 2.939
2013 SCImago Journal Rankings: 1.149
ISI Accession Number ID
Funding AgencyGrant Number
Seed Funding Programme for Applied Research200807160015
China-Japan Feida Union Company Limited
Funding Information:

The research study was supported in part by a grant from Seed Funding Programme for Applied Research (no.: 200807160015) and contract research funding from China-Japan Feida Union Company Limited.

References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChu, ESMen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorCheung, HPen_HK
dc.contributor.authorWong, KLen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorNg, TBen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2011-07-27T02:12:56Z-
dc.date.available2011-07-27T02:12:56Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 403-413en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136308-
dc.description.abstractAim of study: This study aimed to elucidate and compare the anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive components namely butanol (BU), ethyl-acetate (EA) and aqueous (WA) fractions on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mouse xenografts). Materials and methods: The anti-proliferative effects of TXL and its bioactive components in HT-29 cells were determined by MTT assay. Their modulations on the potential angiogenic and metastatic marker expressions on HT-29 cells and xenografts were investigated by real-time PCR and Western blot at transcriptional and translational levels, respectively. For the in vitro study, migration abilities of HT-29 cells were determined using wound healing assay. For the in vivo study, daily measurements of the tumor size and volume of the xenografts were also performed. Results: TXL, BU, EA and WA effectively inhibited the proliferation of HT-29 cells in a dose- and time-dependent manner. The IC 50 value of TXL on HT-29 cells was obtained after incubation with 1% (v/v) TXL for 4 h; whereas IC 50 values were obtained for the following bioactive components: BU at 1.25% (v/v); EA at 5% (v/v); and WA at 0.3125% (v/v). It was found that 1% (v/v) TXL significantly down-regulated MMP2 and MMP7 expression at both transcriptional and translational levels and it reduced MMP9 and VEGF protein expression in vitro. TXL decreased the metastatic ability of HT-29 cells as demonstrated by wound healing assay. TXL and its bioactive fractions caused no significant changes in the body weight indicating lack of toxicity to the xenografts. Conclusions: In summary, TXL multi-targeted to down-regulate the metastatic markers in both in vitro and in vivo models. However, the effects of its bioactive fractions were not obvious. This study profoundly elucidated the anti-proliferative mechanism of TXL, which is vital for the development of future anti-cancer regime in Chinese medicinal formulations. © 2011 Elsevier Ireland Ltd All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectAnti-metastaticen_HK
dc.subjectChinese medicinal formulationen_HK
dc.subjectColorectal canceren_HK
dc.subjectMatrix metalloproteinasesen_HK
dc.subjectTian-Xian liquiden_HK
dc.subjectVascular endothelial growth factoren_HK
dc.titleDifferential effects of anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive fractions on human colorectal cancer modelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=137&issue=1&spage=403&epage=413&date=2011&atitle=Differential+effects+of+anti-metastatic+mechanism+of+Tian-Xian+Liquid+(TXL)+and+its+bioactive+fractions+on+human+colorectal+cancer+models-
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2011.05.035en_HK
dc.identifier.pmid21669277en_HK
dc.identifier.scopuseid_2-s2.0-80052035168en_HK
dc.identifier.hkuros188145en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052035168&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume137en_HK
dc.identifier.issue1en_HK
dc.identifier.spage403en_HK
dc.identifier.epage413en_HK
dc.identifier.isiWOS:000295236700050-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridChu, ESM=13807807000en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridCheung, HP=37033470100en_HK
dc.identifier.scopusauthoridWong, KL=47861480700en_HK
dc.identifier.scopusauthoridLiu, Q=36238108000en_HK
dc.identifier.scopusauthoridNg, TB=35311803300en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK

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