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Article: Aflatoxin B1 - a potential endocrine disruptor - up-regulates CYP19A1 in JEG-3 cells

TitleAflatoxin B1 - a potential endocrine disruptor - up-regulates CYP19A1 in JEG-3 cells
Authors
KeywordsAflatoxicol
Aflatoxin B1
Aromatase
Endocrine disruptor
JEG-3
Placenta
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxlet
Citation
Toxicology Letters, 2011, v. 202 n. 3, p. 161-167 How to Cite?
AbstractPrevious studies have indicated that aromatase (CYP19A1) is involved in the metabolism of aflatoxin B1 (AFB1). We hypothesized that exposure to AFB1 contaminated food during pregnancy could disrupt the normal production of steroid hormones in placenta. We examined the capability of AFB1 exposure to disrupt CYP19A1 expression as a putative endocrine disrupter, and to investigate the metabolism of AFB1 by CYP19A1. JEG-3 cells, as model for placental cells, were exposed alone and in combination to AFB1 and estrogen receptor ligands for 24-96. h. AFB1 (0.3-1.0μM) induced the expression of CYP19A1 by 163%-339% compared to control at the 96. h time point, although no induction was observed at 24. h. AFB1 concentrations higher than 1μM were cytotoxic to JEG-3 cells, and the cytotoxicity was inhibited by the aromatase inhibitor, finrozole. AFB1 was metabolized to aflatoxicol (AFL) by JEG-3 cells and CYP19A1 recombinant protein. AFL formation was partially inhibited by addition of tamoxifen and finrozole to the JEG-3 cells. AFB1 had no effect on the expression of CYP1A2 and CYP3A4 in JEG-3 cells. These results reveal that AFB1 can affect the expression of aromatase enzyme, indicating that chronic exposure to AFB1 may cause endocrine disruption in the foetoplacental unit. © 2011 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/136249
ISSN
2015 Impact Factor: 3.522
2015 SCImago Journal Rankings: 1.267
ISI Accession Number ID
Funding AgencyGrant Number
Academy of Finland122859/2007
Finnish Funding Agency for Technology and Innovation40225/2008
Funding Information:

We would like to thank Mrs. Pirjo Hanninen for her skilful laboratory assistance. This work was supported by The Academy of Finland [122859/2007] and by The Finnish Funding Agency for Technology and Innovation [40225/2008].

References

 

DC FieldValueLanguage
dc.contributor.authorStorvik, Men_HK
dc.contributor.authorHuuskonen, Pen_HK
dc.contributor.authorKyllönen, Ten_HK
dc.contributor.authorLehtonen, Sen_HK
dc.contributor.authorElNezami, Hen_HK
dc.contributor.authorAuriola, Sen_HK
dc.contributor.authorPasanen, Men_HK
dc.date.accessioned2011-07-27T02:11:40Z-
dc.date.available2011-07-27T02:11:40Z-
dc.date.issued2011en_HK
dc.identifier.citationToxicology Letters, 2011, v. 202 n. 3, p. 161-167en_HK
dc.identifier.issn0378-4274en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136249-
dc.description.abstractPrevious studies have indicated that aromatase (CYP19A1) is involved in the metabolism of aflatoxin B1 (AFB1). We hypothesized that exposure to AFB1 contaminated food during pregnancy could disrupt the normal production of steroid hormones in placenta. We examined the capability of AFB1 exposure to disrupt CYP19A1 expression as a putative endocrine disrupter, and to investigate the metabolism of AFB1 by CYP19A1. JEG-3 cells, as model for placental cells, were exposed alone and in combination to AFB1 and estrogen receptor ligands for 24-96. h. AFB1 (0.3-1.0μM) induced the expression of CYP19A1 by 163%-339% compared to control at the 96. h time point, although no induction was observed at 24. h. AFB1 concentrations higher than 1μM were cytotoxic to JEG-3 cells, and the cytotoxicity was inhibited by the aromatase inhibitor, finrozole. AFB1 was metabolized to aflatoxicol (AFL) by JEG-3 cells and CYP19A1 recombinant protein. AFL formation was partially inhibited by addition of tamoxifen and finrozole to the JEG-3 cells. AFB1 had no effect on the expression of CYP1A2 and CYP3A4 in JEG-3 cells. These results reveal that AFB1 can affect the expression of aromatase enzyme, indicating that chronic exposure to AFB1 may cause endocrine disruption in the foetoplacental unit. © 2011 Elsevier Ireland Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxleten_HK
dc.relation.ispartofToxicology Lettersen_HK
dc.subjectAflatoxicolen_HK
dc.subjectAflatoxin B1en_HK
dc.subjectAromataseen_HK
dc.subjectEndocrine disruptoren_HK
dc.subjectJEG-3en_HK
dc.subjectPlacentaen_HK
dc.subject.meshAflatoxin B1 - metabolism - toxicity-
dc.subject.meshAromatase - genetics - metabolism-
dc.subject.meshEndocrine Disruptors - toxicity-
dc.subject.meshPoisons - metabolism - toxicity-
dc.subject.meshTrophoblasts - drug effects - enzymology - pathology-
dc.titleAflatoxin B1 - a potential endocrine disruptor - up-regulates CYP19A1 in JEG-3 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_HK
dc.identifier.authorityElNezami, H=rp00694en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.toxlet.2011.01.028en_HK
dc.identifier.pmid21296134-
dc.identifier.scopuseid_2-s2.0-79954599433en_HK
dc.identifier.hkuros188045en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79954599433&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume202en_HK
dc.identifier.issue3en_HK
dc.identifier.spage161en_HK
dc.identifier.epage167en_HK
dc.identifier.isiWOS:000290778600002-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridStorvik, M=8929225600en_HK
dc.identifier.scopusauthoridHuuskonen, P=41661617800en_HK
dc.identifier.scopusauthoridKyllönen, T=41661701900en_HK
dc.identifier.scopusauthoridLehtonen, S=23091344600en_HK
dc.identifier.scopusauthoridElNezami, H=6603690577en_HK
dc.identifier.scopusauthoridAuriola, S=7003495721en_HK
dc.identifier.scopusauthoridPasanen, M=7004834175en_HK
dc.identifier.citeulike8800609-

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