Article: Validation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay

File Download
  • No File Attached
Links for fulltext
(May Require Subscription)
Supplementary

  • Basic View
  • Metadata View
  • XML View
TitleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
AuthorsWu, JJ2
Mak, YL2
Murphy, MB2
Lam, JCW2
Chan, WH2
Wang, M1
Chan, LL2
Lam, PKS2
KeywordsAccelerated solvent extraction
Ciguatera fish poisoning
Ciguatoxin
HPLC-MS/MS
Mouse neuroblastoma assay
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
CitationAnalytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00216-011-4977-4
AbstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.
ISSN1618-2642
2011 Impact Factor: 3.778
2011 SCImago Journal Rankings: 0.284
DOIhttp://dx.doi.org/10.1007/s00216-011-4977-4
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWu, JJ
dc.contributor.authorMak, YL
dc.contributor.authorMurphy, MB
dc.contributor.authorLam, JCW
dc.contributor.authorChan, WH
dc.contributor.authorWang, M
dc.contributor.authorChan, LL
dc.contributor.authorLam, PKS
dc.date.accessioned2011-07-27T02:11:38Z
dc.date.available2011-07-27T02:11:38Z
dc.date.issued2011
dc.description.abstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00216-011-4977-4
dc.identifier.citeulike9201261
dc.identifier.doihttp://dx.doi.org/10.1007/s00216-011-4977-4
dc.identifier.epage3175
dc.identifier.hkuros186784
dc.identifier.isiWOS:000291037800048
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaCityU3/CRF/08
Funding Information:

We thank Ms. Rachel P. P. Wong (School of Biological Science, The University of Hong Kong) for identification of fish species and Asia Pacific Application Support Center of AB Sciex for providing 5500 QTRAP and technical assistance. The work described in this paper was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (CityU3/CRF/08).

dc.identifier.issn1618-2642
2011 Impact Factor: 3.778
2011 SCImago Journal Rankings: 0.284
dc.identifier.issue9
dc.identifier.openurl
dc.identifier.pmid21505950
dc.identifier.scopuseid_2-s2.0-79958129600
dc.identifier.spage3165
dc.identifier.urihttp://hdl.handle.net/10722/136243
dc.identifier.volume400
dc.languageeng
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
dc.publisher.placeGermany
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
dc.relation.referencesReferences in Scopus
dc.rightsThe original publication is available at www.springerlink.com
dc.subjectAccelerated solvent extraction
dc.subjectCiguatera fish poisoning
dc.subjectCiguatoxin
dc.subjectHPLC-MS/MS
dc.subjectMouse neuroblastoma assay
dc.titleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong