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Article: Validation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay

TitleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
Authors
KeywordsAccelerated solvent extraction
Ciguatera fish poisoning
Ciguatoxin
HPLC-MS/MS
Mouse neuroblastoma assay
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175 How to Cite?
AbstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/136243
ISSN
2014 Impact Factor: 3.436
2014 SCImago Journal Rankings: 1.027
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaCityU3/CRF/08
Funding Information:

We thank Ms. Rachel P. P. Wong (School of Biological Science, The University of Hong Kong) for identification of fish species and Asia Pacific Application Support Center of AB Sciex for providing 5500 QTRAP and technical assistance. The work described in this paper was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (CityU3/CRF/08).

References

 

DC FieldValueLanguage
dc.contributor.authorWu, JJen_HK
dc.contributor.authorMak, YLen_HK
dc.contributor.authorMurphy, MBen_HK
dc.contributor.authorLam, JCWen_HK
dc.contributor.authorChan, WHen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorChan, LLen_HK
dc.contributor.authorLam, PKSen_HK
dc.date.accessioned2011-07-27T02:11:38Z-
dc.date.available2011-07-27T02:11:38Z-
dc.date.issued2011en_HK
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175en_HK
dc.identifier.issn1618-2642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/136243-
dc.description.abstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectAccelerated solvent extractionen_HK
dc.subjectCiguatera fish poisoningen_HK
dc.subjectCiguatoxinen_HK
dc.subjectHPLC-MS/MSen_HK
dc.subjectMouse neuroblastoma assayen_HK
dc.titleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1618-2642&volume=400&issue=9&spage=3165&epage=3175&date=2011&atitle=Validation+of+an+accelerated+solvent+extraction+liquid+chromatography-tandem+mass+spectrometry+method+for+Pacific+ciguatoxin-1+in+fish+flesh+and+comparison+with+the+mouse+neuroblastoma+assay-
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00216-011-4977-4en_HK
dc.identifier.pmid21505950-
dc.identifier.scopuseid_2-s2.0-79958129600en_HK
dc.identifier.hkuros186784en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79958129600&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume400en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3165en_HK
dc.identifier.epage3175en_HK
dc.identifier.isiWOS:000291037800048-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridWu, JJ=37103028100en_HK
dc.identifier.scopusauthoridMak, YL=37011332400en_HK
dc.identifier.scopusauthoridMurphy, MB=7403900446en_HK
dc.identifier.scopusauthoridLam, JCW=24178129400en_HK
dc.identifier.scopusauthoridChan, WH=34871462100en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.citeulike9201261-

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