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Article: Validation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
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TitleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
 
AuthorsWu, JJ2
Mak, YL2
Murphy, MB2
Lam, JCW2
Chan, WH2
Wang, M1
Chan, LL2
Lam, PKS2
 
KeywordsAccelerated solvent extraction
Ciguatera fish poisoning
Ciguatoxin
HPLC-MS/MS
Mouse neuroblastoma assay
 
Issue Date2011
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
 
CitationAnalytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00216-011-4977-4
 
AbstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.
 
ISSN1618-2642
2013 Impact Factor: 3.578
 
DOIhttp://dx.doi.org/10.1007/s00216-011-4977-4
 
ISI Accession Number IDWOS:000291037800048
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaCityU3/CRF/08
Funding Information:

We thank Ms. Rachel P. P. Wong (School of Biological Science, The University of Hong Kong) for identification of fish species and Asia Pacific Application Support Center of AB Sciex for providing 5500 QTRAP and technical assistance. The work described in this paper was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (CityU3/CRF/08).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWu, JJ
 
dc.contributor.authorMak, YL
 
dc.contributor.authorMurphy, MB
 
dc.contributor.authorLam, JCW
 
dc.contributor.authorChan, WH
 
dc.contributor.authorWang, M
 
dc.contributor.authorChan, LL
 
dc.contributor.authorLam, PKS
 
dc.date.accessioned2011-07-27T02:11:38Z
 
dc.date.available2011-07-27T02:11:38Z
 
dc.date.issued2011
 
dc.description.abstractCiguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. © 2011 Springer-Verlag.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2011, v. 400 n. 9, p. 3165-3175 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00216-011-4977-4
 
dc.identifier.citeulike9201261
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00216-011-4977-4
 
dc.identifier.epage3175
 
dc.identifier.hkuros186784
 
dc.identifier.isiWOS:000291037800048
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaCityU3/CRF/08
Funding Information:

We thank Ms. Rachel P. P. Wong (School of Biological Science, The University of Hong Kong) for identification of fish species and Asia Pacific Application Support Center of AB Sciex for providing 5500 QTRAP and technical assistance. The work described in this paper was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (CityU3/CRF/08).

 
dc.identifier.issn1618-2642
2013 Impact Factor: 3.578
 
dc.identifier.issue9
 
dc.identifier.openurl
 
dc.identifier.pmid21505950
 
dc.identifier.scopuseid_2-s2.0-79958129600
 
dc.identifier.spage3165
 
dc.identifier.urihttp://hdl.handle.net/10722/136243
 
dc.identifier.volume400
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe original publication is available at www.springerlink.com
 
dc.subjectAccelerated solvent extraction
 
dc.subjectCiguatera fish poisoning
 
dc.subjectCiguatoxin
 
dc.subjectHPLC-MS/MS
 
dc.subjectMouse neuroblastoma assay
 
dc.titleValidation of an accelerated solvent extraction liquid chromatography- tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong