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Article: Proteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1

TitleProteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1
Authors
KeywordsBrevetoxin
Calcium ion binding
Gill
Medaka fish
Neurotoxic shellfish poisoning
Oryzias melastigma
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2011, v. 104 n. 3-4, p. 211-217 How to Cite?
AbstractAlthough brevetoxins (PbTxs) produced by the marine dinoflagellate Karenia brevis are known to be absorbed across gill membranes and exert their acute toxic effects through an ion-channel mediated pathway in neural tissue, the exact biochemical mechanism concerning PbTxs neurotoxicity in neural tissue and gas-exchange organs has not been well elucidated. In this study, we calculated the LC 50 value of PbTx-1 using the medaka fish model, and presented the molecular responses of sub-acute exposure to PbTx-1 with proteomic method. By adopting two-dimensional electrophoresis, the abundances of 14 and 24 proteins were found to be remarkably altered in the gills and brains, respectively, in response to toxin exposure. Thirteen gill and twenty brain proteins were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis. These proteins could be categorized into diverse functional classes such as cell structure, macromolecule metabolism, signal transduction and neurotransmitter release. These findings can help to elucidate the possible pathways by which aquatic toxins affect marine organisms within target organs. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/136242
ISSN
2014 Impact Factor: 3.451
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTian, Len_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorWang, Den_HK
dc.contributor.authorChou, HNen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorChan, LLen_HK
dc.date.accessioned2011-07-27T02:11:37Z-
dc.date.available2011-07-27T02:11:37Z-
dc.date.issued2011en_HK
dc.identifier.citationAquatic Toxicology, 2011, v. 104 n. 3-4, p. 211-217en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/136242-
dc.description.abstractAlthough brevetoxins (PbTxs) produced by the marine dinoflagellate Karenia brevis are known to be absorbed across gill membranes and exert their acute toxic effects through an ion-channel mediated pathway in neural tissue, the exact biochemical mechanism concerning PbTxs neurotoxicity in neural tissue and gas-exchange organs has not been well elucidated. In this study, we calculated the LC 50 value of PbTx-1 using the medaka fish model, and presented the molecular responses of sub-acute exposure to PbTx-1 with proteomic method. By adopting two-dimensional electrophoresis, the abundances of 14 and 24 proteins were found to be remarkably altered in the gills and brains, respectively, in response to toxin exposure. Thirteen gill and twenty brain proteins were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis. These proteins could be categorized into diverse functional classes such as cell structure, macromolecule metabolism, signal transduction and neurotransmitter release. These findings can help to elucidate the possible pathways by which aquatic toxins affect marine organisms within target organs. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectBrevetoxinen_HK
dc.subjectCalcium ion bindingen_HK
dc.subjectGillen_HK
dc.subjectMedaka fishen_HK
dc.subjectNeurotoxic shellfish poisoningen_HK
dc.subjectOryzias melastigmaen_HK
dc.subject.meshBrain - drug effects - metabolism-
dc.subject.meshGills - drug effects - metabolism-
dc.subject.meshMarine Toxins - toxicity-
dc.subject.meshOryzias-
dc.subject.meshWater Pollutants, Chemical - toxicity-
dc.titleProteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1en_HK
dc.typeArticleen_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2011.04.019en_HK
dc.identifier.pmid21632025-
dc.identifier.scopuseid_2-s2.0-79957578276en_HK
dc.identifier.hkuros186782en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957578276&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume104en_HK
dc.identifier.issue3-4en_HK
dc.identifier.spage211en_HK
dc.identifier.epage217en_HK
dc.identifier.isiWOS:000293042100007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridTian, L=52564351600en_HK
dc.identifier.scopusauthoridWang, M=35085660000en_HK
dc.identifier.scopusauthoridLi, X=52563779300en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridWang, D=35335499500en_HK
dc.identifier.scopusauthoridChou, HN=7202063704en_HK
dc.identifier.scopusauthoridLi, Y=36072770000en_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.citeulike9281458-

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