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Article: Proteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1
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TitleProteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1
 
AuthorsTian, L4 3
Wang, M3
Li, X4
Lam, PKS4
Wang, M
Wang, D2
Chou, HN1
Li, Y4
Chan, LL4
 
KeywordsBrevetoxin
Calcium ion binding
Gill
Medaka fish
Neurotoxic shellfish poisoning
Oryzias melastigma
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
CitationAquatic Toxicology, 2011, v. 104 n. 3-4, p. 211-217 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2011.04.019
 
AbstractAlthough brevetoxins (PbTxs) produced by the marine dinoflagellate Karenia brevis are known to be absorbed across gill membranes and exert their acute toxic effects through an ion-channel mediated pathway in neural tissue, the exact biochemical mechanism concerning PbTxs neurotoxicity in neural tissue and gas-exchange organs has not been well elucidated. In this study, we calculated the LC 50 value of PbTx-1 using the medaka fish model, and presented the molecular responses of sub-acute exposure to PbTx-1 with proteomic method. By adopting two-dimensional electrophoresis, the abundances of 14 and 24 proteins were found to be remarkably altered in the gills and brains, respectively, in response to toxin exposure. Thirteen gill and twenty brain proteins were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis. These proteins could be categorized into diverse functional classes such as cell structure, macromolecule metabolism, signal transduction and neurotransmitter release. These findings can help to elucidate the possible pathways by which aquatic toxins affect marine organisms within target organs. © 2011 Elsevier B.V.
 
ISSN0166-445X
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.615
 
DOIhttp://dx.doi.org/10.1016/j.aquatox.2011.04.019
 
ISI Accession Number IDWOS:000293042100007
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTian, L
 
dc.contributor.authorWang, M
 
dc.contributor.authorLi, X
 
dc.contributor.authorLam, PKS
 
dc.contributor.authorWang, M
 
dc.contributor.authorWang, D
 
dc.contributor.authorChou, HN
 
dc.contributor.authorLi, Y
 
dc.contributor.authorChan, LL
 
dc.date.accessioned2011-07-27T02:11:37Z
 
dc.date.available2011-07-27T02:11:37Z
 
dc.date.issued2011
 
dc.description.abstractAlthough brevetoxins (PbTxs) produced by the marine dinoflagellate Karenia brevis are known to be absorbed across gill membranes and exert their acute toxic effects through an ion-channel mediated pathway in neural tissue, the exact biochemical mechanism concerning PbTxs neurotoxicity in neural tissue and gas-exchange organs has not been well elucidated. In this study, we calculated the LC 50 value of PbTx-1 using the medaka fish model, and presented the molecular responses of sub-acute exposure to PbTx-1 with proteomic method. By adopting two-dimensional electrophoresis, the abundances of 14 and 24 proteins were found to be remarkably altered in the gills and brains, respectively, in response to toxin exposure. Thirteen gill and twenty brain proteins were identified using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry analysis. These proteins could be categorized into diverse functional classes such as cell structure, macromolecule metabolism, signal transduction and neurotransmitter release. These findings can help to elucidate the possible pathways by which aquatic toxins affect marine organisms within target organs. © 2011 Elsevier B.V.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAquatic Toxicology, 2011, v. 104 n. 3-4, p. 211-217 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2011.04.019
 
dc.identifier.citeulike9281458
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquatox.2011.04.019
 
dc.identifier.epage217
 
dc.identifier.hkuros186782
 
dc.identifier.isiWOS:000293042100007
 
dc.identifier.issn0166-445X
2012 Impact Factor: 3.73
2012 SCImago Journal Rankings: 1.615
 
dc.identifier.issue3-4
 
dc.identifier.pmid21632025
 
dc.identifier.scopuseid_2-s2.0-79957578276
 
dc.identifier.spage211
 
dc.identifier.urihttp://hdl.handle.net/10722/136242
 
dc.identifier.volume104
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofAquatic Toxicology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBrain - drug effects - metabolism
 
dc.subject.meshGills - drug effects - metabolism
 
dc.subject.meshMarine Toxins - toxicity
 
dc.subject.meshOryzias
 
dc.subject.meshWater Pollutants, Chemical - toxicity
 
dc.subjectBrevetoxin
 
dc.subjectCalcium ion binding
 
dc.subjectGill
 
dc.subjectMedaka fish
 
dc.subjectNeurotoxic shellfish poisoning
 
dc.subjectOryzias melastigma
 
dc.titleProteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1
 
dc.typeArticle
 
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<contributor.author>Wang, M</contributor.author>
<contributor.author>Wang, D</contributor.author>
<contributor.author>Chou, HN</contributor.author>
<contributor.author>Li, Y</contributor.author>
<contributor.author>Chan, LL</contributor.author>
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Author Affiliations
  1. National Taiwan University
  2. Xiamen University
  3. The University of Hong Kong
  4. City University of Hong Kong