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Conference Paper: Developing a new sonoporation treatment model using plants: cellular mechanism analysis

TitleDeveloping a new sonoporation treatment model using plants: cellular mechanism analysis
Authors
KeywordsAcoustic cavitation
Plant cells
Mechanism analysis
Experimental modeling
Issue Date2011
PublisherIEEE. The Journal's web site is located at http://www.ieeexplore.ieee.org/xpl/conhome.jsp?punumber=1000770
Citation
The 2011 IEEE International Ultrasonics Symposium (IUS), Orlando, FL., 18-21 October 2011. In IEEE International Ultrasonics Symposium Proceedings, 2011, p. 616-619 How to Cite?
AbstractPlant cells (BY-2 tobacco) are used in this work as a new cellular model for investigating the biological mechanisms behind sonoporation-induced cytotoxic effects. We show that sonoporation may lead to chronic depolarization of plasma membrane and depolarization of mitochondrial membrane: both of which are known to be linked to a cell’s programmed cell death protocol. These findings are obtained in-vitro from BY-2 cell suspensions that were exposed to pulsed ultrasound for 1 min. (1 MHz ultrasound frequency, 10% duty cycle, 1 kHz pulse repetition frequency, 0.4 or 0.9 MPa peak negative pressure, 1% v/v microbubbles). Two potentio-dependent fluorescence probes (DiBAC4(3) and TMRE) were used to analyze the changes in the plasma membrane and mitochondrial membrane potential of the sonoporated cells at four post-exposure time points (0 h, 2 h, 4 h, 6 h). These changes were monitored by confocal and fluorescence microscopy, and they were quantified using a spectrofluorometer. The membrane depolarization effects are generally found to be more significant at higher peak negative pressures (0.9 MPa in our case).
DescriptionPoster Session P2Aa. a - Bio-effects: no. P2Aa-2
Persistent Identifierhttp://hdl.handle.net/10722/135863
ISBN
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQin, Pen_US
dc.contributor.authorXu, Len_US
dc.contributor.authorZhong, Wen_US
dc.contributor.authorYu, ACHen_US
dc.date.accessioned2011-07-27T01:49:44Z-
dc.date.available2011-07-27T01:49:44Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 IEEE International Ultrasonics Symposium (IUS), Orlando, FL., 18-21 October 2011. In IEEE International Ultrasonics Symposium Proceedings, 2011, p. 616-619en_US
dc.identifier.isbn978-1-4577-1252-4-
dc.identifier.issn1051-0117-
dc.identifier.urihttp://hdl.handle.net/10722/135863-
dc.descriptionPoster Session P2Aa. a - Bio-effects: no. P2Aa-2-
dc.description.abstractPlant cells (BY-2 tobacco) are used in this work as a new cellular model for investigating the biological mechanisms behind sonoporation-induced cytotoxic effects. We show that sonoporation may lead to chronic depolarization of plasma membrane and depolarization of mitochondrial membrane: both of which are known to be linked to a cell’s programmed cell death protocol. These findings are obtained in-vitro from BY-2 cell suspensions that were exposed to pulsed ultrasound for 1 min. (1 MHz ultrasound frequency, 10% duty cycle, 1 kHz pulse repetition frequency, 0.4 or 0.9 MPa peak negative pressure, 1% v/v microbubbles). Two potentio-dependent fluorescence probes (DiBAC4(3) and TMRE) were used to analyze the changes in the plasma membrane and mitochondrial membrane potential of the sonoporated cells at four post-exposure time points (0 h, 2 h, 4 h, 6 h). These changes were monitored by confocal and fluorescence microscopy, and they were quantified using a spectrofluorometer. The membrane depolarization effects are generally found to be more significant at higher peak negative pressures (0.9 MPa in our case).-
dc.languageengen_US
dc.publisherIEEE. The Journal's web site is located at http://www.ieeexplore.ieee.org/xpl/conhome.jsp?punumber=1000770-
dc.relation.ispartofIEEE International Ultrasonics Symposium Proceedingsen_US
dc.rightsIEEE International Ultrasonics Symposium Proceedings. Copyright © IEEE.-
dc.rights©2011 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectAcoustic cavitation-
dc.subjectPlant cells-
dc.subjectMechanism analysis-
dc.subjectExperimental modeling-
dc.titleDeveloping a new sonoporation treatment model using plants: cellular mechanism analysisen_US
dc.typeConference_Paperen_US
dc.identifier.emailQin, P: pqin@hku.hken_US
dc.identifier.emailZhong, W: wjzhong@eee.hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hk-
dc.identifier.authorityYu, ACH=rp00657en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1109/ULTSYM.2011.0149-
dc.identifier.scopuseid_2-s2.0-84869028272-
dc.identifier.hkuros186962en_US
dc.identifier.spage616-
dc.identifier.epage619-
dc.identifier.isiWOS:000309918400147-
dc.publisher.placeUnited States-
dc.description.otherThe 2011 IEEE International Ultrasonics Symposium (IUS), Orlando, FL., 18-21 October 2011. In IEEE International Ultrasonics Symposium Proceedings, 2011, p. 616-619-

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