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Article: A microrna contribution to aberrant ras activation in gastric cancer

TitleA microrna contribution to aberrant ras activation in gastric cancer
Authors
KeywordsEzrin
Gastric cancer
MiR-204
Ras
Issue Date2011
PublisherE-Century Publishing Corporation. The Journal's web site is located at http://www.ajtr.org
Citation
American Journal Of Translational Research, 2011, v. 3 n. 2, p. 209-218 How to Cite?
Abstract
Oncogenic Ras mutations are rare in gastric cancer, indicating that other mechanisms may be responsible for aberrant Ras activation in this type of cancer. Ezrin is critical to Ras activation by remodeling cortical actin cytoskeleton. In this study, we aimed to illustrate the relevance and regulation of ezrin in gastric cancer. Ezrin was upregulated in gastric cancer cells. Ezrin siRNA inhibited Ras activation, cell growth and cell migration. Ezrin overexpression was correlated with a poor outcome of gastric cancer patients (n=150, p<0.01). Cox regression analysis revealed a significant value of ezrin expression in prognosis prediction of gastric cancer (relative risk: 2.37, 95% confidence interval: 1.24-4.56, p<0.01). MiR-204, which was predicted to target ezrin, was downregulated in gastric cancer cells and gastric carcinomas (n=22, p<0.01). MiR-204 inhibited ezrin expression, Ras activation, cell growth and cell migration. Importantly, miR-204 suppressed the expression of luciferase controlled by wild-type but not mutated ezrin 3'-UTR. In conclusion, ezrin is important to Ras activation in gastric cancer. Its upregulation is an independent prognosis prediction factor for gastric cancer. By contributing to ezrin upregulation, miR-204 downregulation represents a novel mechanism for aberrant Ras activation in gastric carcinogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/135563
ISSN
2013 Impact Factor: 3.226
2013 SCImago Journal Rankings: 1.788
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, EKYen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorShin, VYen_HK
dc.contributor.authorZhang, Sen_HK
dc.contributor.authorMorrison, Hen_HK
dc.contributor.authorSun, Jen_HK
dc.contributor.authorNg, EKOen_HK
dc.contributor.authorYu, Jen_HK
dc.contributor.authorJin, Hen_HK
dc.date.accessioned2011-07-27T01:37:11Z-
dc.date.available2011-07-27T01:37:11Z-
dc.date.issued2011en_HK
dc.identifier.citationAmerican Journal Of Translational Research, 2011, v. 3 n. 2, p. 209-218en_HK
dc.identifier.issn1943-8141en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135563-
dc.description.abstractOncogenic Ras mutations are rare in gastric cancer, indicating that other mechanisms may be responsible for aberrant Ras activation in this type of cancer. Ezrin is critical to Ras activation by remodeling cortical actin cytoskeleton. In this study, we aimed to illustrate the relevance and regulation of ezrin in gastric cancer. Ezrin was upregulated in gastric cancer cells. Ezrin siRNA inhibited Ras activation, cell growth and cell migration. Ezrin overexpression was correlated with a poor outcome of gastric cancer patients (n=150, p<0.01). Cox regression analysis revealed a significant value of ezrin expression in prognosis prediction of gastric cancer (relative risk: 2.37, 95% confidence interval: 1.24-4.56, p<0.01). MiR-204, which was predicted to target ezrin, was downregulated in gastric cancer cells and gastric carcinomas (n=22, p<0.01). MiR-204 inhibited ezrin expression, Ras activation, cell growth and cell migration. Importantly, miR-204 suppressed the expression of luciferase controlled by wild-type but not mutated ezrin 3'-UTR. In conclusion, ezrin is important to Ras activation in gastric cancer. Its upregulation is an independent prognosis prediction factor for gastric cancer. By contributing to ezrin upregulation, miR-204 downregulation represents a novel mechanism for aberrant Ras activation in gastric carcinogenesis.en_HK
dc.languageengen_US
dc.publisherE-Century Publishing Corporation. The Journal's web site is located at http://www.ajtr.orgen_HK
dc.relation.ispartofAmerican Journal of Translational Researchen_HK
dc.subjectEzrinen_HK
dc.subjectGastric canceren_HK
dc.subjectMiR-204en_HK
dc.subjectRasen_HK
dc.titleA microrna contribution to aberrant ras activation in gastric canceren_HK
dc.typeArticleen_HK
dc.identifier.emailNg, EKO: ngko@hku.hken_HK
dc.identifier.authorityNg, EKO=rp01364en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid21416062-
dc.identifier.pmcidPMC3056566-
dc.identifier.scopuseid_2-s2.0-79952921572en_HK
dc.identifier.hkuros188344en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952921572&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue2en_HK
dc.identifier.spage209en_HK
dc.identifier.epage218en_HK
dc.identifier.isiWOS:000208694600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLam, EKY=8644138600en_HK
dc.identifier.scopusauthoridWang, X=12763461000en_HK
dc.identifier.scopusauthoridShin, VY=7003491170en_HK
dc.identifier.scopusauthoridZhang, S=44861941700en_HK
dc.identifier.scopusauthoridMorrison, H=35798625800en_HK
dc.identifier.scopusauthoridSun, J=44861988100en_HK
dc.identifier.scopusauthoridNg, EKO=21135553700en_HK
dc.identifier.scopusauthoridYu, J=35351306800en_HK
dc.identifier.scopusauthoridJin, H=24577511700en_HK

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