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Article: Metabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patients

TitleMetabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patients
Authors
KeywordsMetabolomics Analysis
Partial Hepatectomy
Hepatocellular Carcinoma
LC-MS
Issue Date2011
PublisherScientific Research Publishing, Inc. The Journal's web site is located at http://www.scirp.org/journal/ajac/
Citation
American Journal of Analytical Chemistry, 2011, v. 2 n. 2, p. 142-151 How to Cite?
AbstractIn this study, liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOFMS) was employed for investigating the metabolome of the sera collected from hepatocellular carcinoma (HCC) patients before and 3 to 5 months after partial hepatectomy. To investigate the changes in metabolic phenotypes after the hepatic resection, principal components analysis (PCA) and support vector machine (SVM) were performed for the data grouping and classification. Based on the obtained SVM model, mass spectrometry spectra, database searching as well as the confirmation from authentic standards, several differentiating metabolites were tentatively identified. To improve visualization, z-score plot and heat map display were performed, which exhibited the changes in concentration of the metabolites. As a result, depletion of circulating carnitine, reduced amino acid biosynthesis and increased rate of lipid peroxidation were observed. Meanwhile, up-regulation of hypoxanthine indicated that purine metabolism might serve as the salvage pathway. Collectively, the results reflected metabolic responses to surgical operation in HCC patients, suggesting perturbation of energy metabolism may occur in 3 to 5 months after the partial hepatectomy.
Persistent Identifierhttp://hdl.handle.net/10722/135559
ISSN

 

DC FieldValueLanguage
dc.contributor.authorChan, Wen_US
dc.contributor.authorLin, SHen_US
dc.contributor.authorSun, Sen_US
dc.contributor.authorLiu, HDen_US
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorCai, ZWen_US
dc.date.accessioned2011-07-27T01:37:07Z-
dc.date.available2011-07-27T01:37:07Z-
dc.date.issued2011en_US
dc.identifier.citationAmerican Journal of Analytical Chemistry, 2011, v. 2 n. 2, p. 142-151en_US
dc.identifier.issn2156-8251-
dc.identifier.urihttp://hdl.handle.net/10722/135559-
dc.description.abstractIn this study, liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOFMS) was employed for investigating the metabolome of the sera collected from hepatocellular carcinoma (HCC) patients before and 3 to 5 months after partial hepatectomy. To investigate the changes in metabolic phenotypes after the hepatic resection, principal components analysis (PCA) and support vector machine (SVM) were performed for the data grouping and classification. Based on the obtained SVM model, mass spectrometry spectra, database searching as well as the confirmation from authentic standards, several differentiating metabolites were tentatively identified. To improve visualization, z-score plot and heat map display were performed, which exhibited the changes in concentration of the metabolites. As a result, depletion of circulating carnitine, reduced amino acid biosynthesis and increased rate of lipid peroxidation were observed. Meanwhile, up-regulation of hypoxanthine indicated that purine metabolism might serve as the salvage pathway. Collectively, the results reflected metabolic responses to surgical operation in HCC patients, suggesting perturbation of energy metabolism may occur in 3 to 5 months after the partial hepatectomy.-
dc.languageengen_US
dc.publisherScientific Research Publishing, Inc. The Journal's web site is located at http://www.scirp.org/journal/ajac/-
dc.relation.ispartofAmerican Journal of Analytical Chemistryen_US
dc.subjectMetabolomics Analysis-
dc.subjectPartial Hepatectomy-
dc.subjectHepatocellular Carcinoma-
dc.subjectLC-MS-
dc.titleMetabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patientsen_US
dc.typeArticleen_US
dc.identifier.emailSun, S: ssun@hku.hken_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4236/ajac.2011.22016-
dc.identifier.hkuros188211en_US
dc.identifier.volume2en_US
dc.identifier.issue2-
dc.identifier.spage142en_US
dc.identifier.epage151en_US
dc.publisher.placeUnited States-

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