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Article: Metabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patients
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TitleMetabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patients
 
AuthorsChan, W
Lin, SH
Sun, S
Liu, HD
Luk, JMC
Cai, ZW
 
KeywordsMetabolomics Analysis
Partial Hepatectomy
Hepatocellular Carcinoma
LC-MS
 
Issue Date2011
 
PublisherScientific Research Publishing, Inc. The Journal's web site is located at http://www.scirp.org/journal/ajac/
 
CitationAmerican Journal of Analytical Chemistry, 2011, v. 2 n. 2, p. 142-151 [How to Cite?]
DOI: http://dx.doi.org/10.4236/ajac.2011.22016
 
AbstractIn this study, liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOFMS) was employed for investigating the metabolome of the sera collected from hepatocellular carcinoma (HCC) patients before and 3 to 5 months after partial hepatectomy. To investigate the changes in metabolic phenotypes after the hepatic resection, principal components analysis (PCA) and support vector machine (SVM) were performed for the data grouping and classification. Based on the obtained SVM model, mass spectrometry spectra, database searching as well as the confirmation from authentic standards, several differentiating metabolites were tentatively identified. To improve visualization, z-score plot and heat map display were performed, which exhibited the changes in concentration of the metabolites. As a result, depletion of circulating carnitine, reduced amino acid biosynthesis and increased rate of lipid peroxidation were observed. Meanwhile, up-regulation of hypoxanthine indicated that purine metabolism might serve as the salvage pathway. Collectively, the results reflected metabolic responses to surgical operation in HCC patients, suggesting perturbation of energy metabolism may occur in 3 to 5 months after the partial hepatectomy.
 
DescriptionOpen Access Journal
 
ISSN2156-8251
 
DOIhttp://dx.doi.org/10.4236/ajac.2011.22016
 
DC FieldValue
dc.contributor.authorChan, W
 
dc.contributor.authorLin, SH
 
dc.contributor.authorSun, S
 
dc.contributor.authorLiu, HD
 
dc.contributor.authorLuk, JMC
 
dc.contributor.authorCai, ZW
 
dc.date.accessioned2011-07-27T01:37:07Z
 
dc.date.available2011-07-27T01:37:07Z
 
dc.date.issued2011
 
dc.description.abstractIn this study, liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOFMS) was employed for investigating the metabolome of the sera collected from hepatocellular carcinoma (HCC) patients before and 3 to 5 months after partial hepatectomy. To investigate the changes in metabolic phenotypes after the hepatic resection, principal components analysis (PCA) and support vector machine (SVM) were performed for the data grouping and classification. Based on the obtained SVM model, mass spectrometry spectra, database searching as well as the confirmation from authentic standards, several differentiating metabolites were tentatively identified. To improve visualization, z-score plot and heat map display were performed, which exhibited the changes in concentration of the metabolites. As a result, depletion of circulating carnitine, reduced amino acid biosynthesis and increased rate of lipid peroxidation were observed. Meanwhile, up-regulation of hypoxanthine indicated that purine metabolism might serve as the salvage pathway. Collectively, the results reflected metabolic responses to surgical operation in HCC patients, suggesting perturbation of energy metabolism may occur in 3 to 5 months after the partial hepatectomy.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionOpen Access Journal
 
dc.identifier.citationAmerican Journal of Analytical Chemistry, 2011, v. 2 n. 2, p. 142-151 [How to Cite?]
DOI: http://dx.doi.org/10.4236/ajac.2011.22016
 
dc.identifier.doihttp://dx.doi.org/10.4236/ajac.2011.22016
 
dc.identifier.epage151
 
dc.identifier.hkuros188211
 
dc.identifier.issn2156-8251
 
dc.identifier.issue2
 
dc.identifier.spage142
 
dc.identifier.urihttp://hdl.handle.net/10722/135559
 
dc.identifier.volume2
 
dc.languageeng
 
dc.publisherScientific Research Publishing, Inc. The Journal's web site is located at http://www.scirp.org/journal/ajac/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Analytical Chemistry
 
dc.subjectMetabolomics Analysis
 
dc.subjectPartial Hepatectomy
 
dc.subjectHepatocellular Carcinoma
 
dc.subjectLC-MS
 
dc.titleMetabolomics analysis of the responses to partial hepatectomy in hepatocellular carcinoma patients
 
dc.typeArticle
 
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<contributor.author>Lin, SH</contributor.author>
<contributor.author>Sun, S</contributor.author>
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<contributor.author>Luk, JMC</contributor.author>
<contributor.author>Cai, ZW</contributor.author>
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