Article: Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris
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- Publisher Website: 10.1016/j.phymed.2010.07.010
- Scopus: eid_2-s2.0-79952737572
- PMID: 20739167
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| Title | Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris | ||||
|---|---|---|---|---|---|
| Authors | Wong, JH3 Ng, TB3 Wang, H4 Sze, SCW1 Zhang, KY1 Li, Q2 Lu, X3 | ||||
| Keywords | Antifungal Cordyceps Isolation | ||||
| Issue Date | 2011 | ||||
| Publisher | Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed | ||||
| Citation | Phytomedicine, 2011, v. 18 n. 5, p. 387-392 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.phymed.2010.07.010 | ||||
| Abstract | Cordymin, an antifungal peptide with a molecular mass of 10,906 Da and an N-terminal amino acid sequence distinct from those of previously reported proteins, was purified from the medicinal mushroom Cordyceps militaris. The isolation protocol comprised ion exchange chromatography of the aqueous extract on SP-Sepharose and Mono S and gel filtration on Superdex 75 by a fast protein liquid chromatography system. Cordymin was adsorbed on both cation exchangers. The peptide inhibited mycelial growth in Bipolaris maydis, Mycosphaerella arachidicola, Rhizoctonia solani and Candida albicans with an IC 50 of 50 μM, 10 μM, 80 μM, and 0.75 mM, respectively. However, there was no effect on Aspergillus fumigatus, Fusarium oxysporum and Valsa mali when tested up to 2 mM. The antifungal activity of the peptide was stable up to 100 °C and in the pH range 6-13, and unaffected by 10 mM Zn 2+ and 10 mM Mg 2+. Cordymin inhibited HIV-1 reverse transcriptase with an IC 50 of 55 μM. Cordymin displayed antiproliferative activity toward breast cancer cells (MCF-7) but there was no effect on colon cancer cells (HT-29). There was no mitogenic activity toward mouse spleen cells and no nitric oxide inducing activity toward mouse macrophages when tested up to 1 mM. © 2010 Elsevier GmbH. | ||||
| ISSN | 0944-7113 2011 Impact Factor: 3.268 2011 SCImago Journal Rankings: 0.133 | ||||
| DOI | http://dx.doi.org/10.1016/j.phymed.2010.07.010 | ||||
| ISI Accession Number ID | WOS:000290073100010
Funding Information: The award of a direct grant from the Medicine Panel, CUHK Research Committee is gratefully acknowledged. | ||||
| References | References in Scopus |
| dc.contributor.author | Wong, JH | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Ng, TB | ||||
| dc.contributor.author | Wang, H | ||||
| dc.contributor.author | Sze, SCW | ||||
| dc.contributor.author | Zhang, KY | ||||
| dc.contributor.author | Li, Q | ||||
| dc.contributor.author | Lu, X | ||||
| dc.date.accessioned | 2011-07-27T01:35:06Z | ||||
| dc.date.available | 2011-07-27T01:35:06Z | ||||
| dc.date.issued | 2011 | ||||
| dc.description.abstract | Cordymin, an antifungal peptide with a molecular mass of 10,906 Da and an N-terminal amino acid sequence distinct from those of previously reported proteins, was purified from the medicinal mushroom Cordyceps militaris. The isolation protocol comprised ion exchange chromatography of the aqueous extract on SP-Sepharose and Mono S and gel filtration on Superdex 75 by a fast protein liquid chromatography system. Cordymin was adsorbed on both cation exchangers. The peptide inhibited mycelial growth in Bipolaris maydis, Mycosphaerella arachidicola, Rhizoctonia solani and Candida albicans with an IC 50 of 50 μM, 10 μM, 80 μM, and 0.75 mM, respectively. However, there was no effect on Aspergillus fumigatus, Fusarium oxysporum and Valsa mali when tested up to 2 mM. The antifungal activity of the peptide was stable up to 100 °C and in the pH range 6-13, and unaffected by 10 mM Zn 2+ and 10 mM Mg 2+. Cordymin inhibited HIV-1 reverse transcriptase with an IC 50 of 55 μM. Cordymin displayed antiproliferative activity toward breast cancer cells (MCF-7) but there was no effect on colon cancer cells (HT-29). There was no mitogenic activity toward mouse spleen cells and no nitric oxide inducing activity toward mouse macrophages when tested up to 1 mM. © 2010 Elsevier GmbH. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Phytomedicine, 2011, v. 18 n. 5, p. 387-392 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.phymed.2010.07.010 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.phymed.2010.07.010 | ||||
| dc.identifier.epage | 392 | ||||
| dc.identifier.hkuros | 188258 | ||||
| dc.identifier.isi | WOS:000290073100010
Funding Information: The award of a direct grant from the Medicine Panel, CUHK Research Committee is gratefully acknowledged. | ||||
| dc.identifier.issn | 0944-7113 2011 Impact Factor: 3.268 2011 SCImago Journal Rankings: 0.133 | ||||
| dc.identifier.issue | 5 | ||||
| dc.identifier.openurl | | ||||
| dc.identifier.pmid | 20739167 | ||||
| dc.identifier.scopus | eid_2-s2.0-79952737572 | ||||
| dc.identifier.spage | 387 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/135436 | ||||
| dc.identifier.volume | 18 | ||||
| dc.language | eng | ||||
| dc.publisher | Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed | ||||
| dc.publisher.place | Germany | ||||
| dc.relation.ispartof | Phytomedicine | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject | Antifungal | ||||
| dc.subject | Cordyceps | ||||
| dc.subject | Isolation | ||||
| dc.title | Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The University of Hong Kong
- Chinese University of Hong Kong
- China Agricultural University