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Article: Expression of vesicular glutamate transporters in peripheral vestibular structures and vestibular nuclear complex of rat

TitleExpression of vesicular glutamate transporters in peripheral vestibular structures and vestibular nuclear complex of rat
Authors
KeywordsGanglionectomy
Hair cell
Immunohistochemistry
Rat
Vesicular glutamate transporter
Vestibular nucleus
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 2011, v. 173, p. 179-189 How to Cite?
AbstractGlutamate transmission from vestibular end organs to central vestibular nuclear complex (VNC) plays important role in transferring sensory information about head position and movements. Three isoforms of vesicular glutamate transporters (VGLUTs) have been considered so far the most specific markers for glutamatergic neurons/cells. In this study, VGLUT1 and VGLUT2 were immunohistochemically localized to axon terminals in VNC and somata of vestibular primary afferents in association with their central and peripheral axon endings, and VGLUT1 and VGLUT3 were co-localized to hair cells of otolith maculae and cristae ampullaris. VGLUT1 and VGLUT2 defined three subsets of Scarpa's neurons (vestibular ganglionic neurons): those co-expressing VGLUT1 and VGLUT2 or expressing only VGLUT2, and those expressing neither. In addition, many neurons located in all vestibular subnuclei were observed to contain hybridized signals for VGLUT2 mRNA and a few VNC neurons, mostly scattered in medial vestibular nucleus (MVe), displayed VGLUT1 mRNA labelling. Following unilateral ganglionectomy, asymmetries of VGLUT1-immunoreactivity (ir) and VGLUT2-ir occurred between two VNCs, indicating that the VNC terminals containing VGLUT1 and/or VGLUT2 are partly of peripheral origin. The present data indicate that the constituent cells/neurons along the vestibular pathway selectively apply VGLUT isoforms to transport glutamate into synaptic vesicles for glutamate transmission. © 2011 IBRO.
Persistent Identifierhttp://hdl.handle.net/10722/135381
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China81071105
81070900
30971123
31010103909
30771133
Funding Information:

This work is supported by the National Natural Science Foundation of China (No. 81071105, 81070900, 30971123, 31010103909 and 30771133).

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, FXen_HK
dc.contributor.authorPang, YWen_HK
dc.contributor.authorZhang, MMen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorDong, YLen_HK
dc.contributor.authorLai, CHen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorLi, JLen_HK
dc.contributor.authorLi, YQen_HK
dc.date.accessioned2011-07-27T01:34:25Z-
dc.date.available2011-07-27T01:34:25Z-
dc.date.issued2011en_HK
dc.identifier.citationNeuroscience, 2011, v. 173, p. 179-189en_HK
dc.identifier.issn0306-4522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135381-
dc.description.abstractGlutamate transmission from vestibular end organs to central vestibular nuclear complex (VNC) plays important role in transferring sensory information about head position and movements. Three isoforms of vesicular glutamate transporters (VGLUTs) have been considered so far the most specific markers for glutamatergic neurons/cells. In this study, VGLUT1 and VGLUT2 were immunohistochemically localized to axon terminals in VNC and somata of vestibular primary afferents in association with their central and peripheral axon endings, and VGLUT1 and VGLUT3 were co-localized to hair cells of otolith maculae and cristae ampullaris. VGLUT1 and VGLUT2 defined three subsets of Scarpa's neurons (vestibular ganglionic neurons): those co-expressing VGLUT1 and VGLUT2 or expressing only VGLUT2, and those expressing neither. In addition, many neurons located in all vestibular subnuclei were observed to contain hybridized signals for VGLUT2 mRNA and a few VNC neurons, mostly scattered in medial vestibular nucleus (MVe), displayed VGLUT1 mRNA labelling. Following unilateral ganglionectomy, asymmetries of VGLUT1-immunoreactivity (ir) and VGLUT2-ir occurred between two VNCs, indicating that the VNC terminals containing VGLUT1 and/or VGLUT2 are partly of peripheral origin. The present data indicate that the constituent cells/neurons along the vestibular pathway selectively apply VGLUT isoforms to transport glutamate into synaptic vesicles for glutamate transmission. © 2011 IBRO.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_HK
dc.relation.ispartofNeuroscienceen_HK
dc.subjectGanglionectomyen_HK
dc.subjectHair cellen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectRaten_HK
dc.subjectVesicular glutamate transporteren_HK
dc.subjectVestibular nucleusen_HK
dc.subject.meshAfferent Pathways - metabolism-
dc.subject.meshNeurons - metabolism-
dc.subject.meshVesicular Glutamate Transport Proteins - biosynthesis-
dc.subject.meshVestibular Nuclei - metabolism-
dc.subject.meshVestibule, Labyrinth - metabolism-
dc.titleExpression of vesicular glutamate transporters in peripheral vestibular structures and vestibular nuclear complex of raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0306-4522&volume=173&spage=179&epage=189&date=2011&atitle=Expression+of+vesicular+glutamate+transporters+in+peripheral+vestibular+structures+and+vestibular+nuclear+complex+of+rat-
dc.identifier.emailLai, CH: chlaib@hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityLai, CH=rp00396en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neuroscience.2010.11.013en_HK
dc.identifier.pmid21081152-
dc.identifier.scopuseid_2-s2.0-78650676720en_HK
dc.identifier.hkuros187481en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650676720&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume173en_HK
dc.identifier.spage179en_HK
dc.identifier.epage189en_HK
dc.identifier.isiWOS:000286487000016-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, FX=9241493900en_HK
dc.identifier.scopusauthoridPang, YW=12809377800en_HK
dc.identifier.scopusauthoridZhang, MM=8271300800en_HK
dc.identifier.scopusauthoridZhang, T=37118216200en_HK
dc.identifier.scopusauthoridDong, YL=7403390945en_HK
dc.identifier.scopusauthoridLai, CH=7403086597en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridLi, JL=36068047500en_HK
dc.identifier.scopusauthoridLi, YQ=26661169500en_HK
dc.identifier.citeulike8264924-

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