File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The involvement of microtubules and actin filaments in the intracellular transport of non-viral gene delivery system

TitleThe involvement of microtubules and actin filaments in the intracellular transport of non-viral gene delivery system
Authors
KeywordsActin filaments
confocal microscopy
endocytosis
gene delivery
intracellular trafficking
microtubules
plasmid DNA
polymer
transfection
Issue Date2011
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1061186x.asp
Citation
Journal Of Drug Targeting, 2011, v. 19 n. 1, p. 56-66 How to Cite?
AbstractIt is known that two cytoskeleton components, microtubules and actins filaments, are required for efficient endocytosis. The relative importance of these two components in the cellular uptake of 2-(dimethylamino)ethyl methacrylate (DMAEMA)-DNA polyplexes was investigated in this study by applying microtubule depolymerising agent, colchicine, and actin polymerising inhibitor, cytochalasin D, in a cell transfection study. The effect of colchicine on transfection efficiency of polyplexes was found to be a time-dependent phenomenon, whereby the level of gene expression was inhibited at early stage, presumably to the disruption of a transport of vesicles along microtubules by colchicine. As time progressed, the level of gene expression was significantly enhanced relative to the control, possibly due to the failure in transport of vesicles from endosomes to late lysosomes, or due to the breakdown of nuclear membrane when mitosis was arrested at metaphase by colchicine. On the other hand, transfection efficiency was significantly reduced at all time points by cytochalasin D, which is considered to primarily affects invagination of vesicles at the early stage of endocytosis by inhibiting actin polymerisation. Further investigation to identify the endocytotic route of DMAEMA polyplexes was conducted applying clathrin- and caveolae- pathways inhibitors in cell transfection study. The results indicate that DMAEMA polyplexes were internalized primarily through clathrin-mediated pathway, with a minor fraction possibly entering cells via a caveolae-mediated pathway. © 2011 Informa UK, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135354
ISSN
2015 Impact Factor: 2.821
2015 SCImago Journal Rankings: 0.749
ISI Accession Number ID
Funding AgencyGrant Number
Biocompatibles (UK)
University of Nottingham
Funding Information:

The authors thank Biocompatibles (UK) and the University of Nottingham for studentship funding.

References

 

DC FieldValueLanguage
dc.contributor.authorLam, JKWen_HK
dc.contributor.authorArmes, SPen_HK
dc.contributor.authorStolnik, Sen_HK
dc.date.accessioned2011-07-27T01:34:00Z-
dc.date.available2011-07-27T01:34:00Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Drug Targeting, 2011, v. 19 n. 1, p. 56-66en_HK
dc.identifier.issn1061-186Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135354-
dc.description.abstractIt is known that two cytoskeleton components, microtubules and actins filaments, are required for efficient endocytosis. The relative importance of these two components in the cellular uptake of 2-(dimethylamino)ethyl methacrylate (DMAEMA)-DNA polyplexes was investigated in this study by applying microtubule depolymerising agent, colchicine, and actin polymerising inhibitor, cytochalasin D, in a cell transfection study. The effect of colchicine on transfection efficiency of polyplexes was found to be a time-dependent phenomenon, whereby the level of gene expression was inhibited at early stage, presumably to the disruption of a transport of vesicles along microtubules by colchicine. As time progressed, the level of gene expression was significantly enhanced relative to the control, possibly due to the failure in transport of vesicles from endosomes to late lysosomes, or due to the breakdown of nuclear membrane when mitosis was arrested at metaphase by colchicine. On the other hand, transfection efficiency was significantly reduced at all time points by cytochalasin D, which is considered to primarily affects invagination of vesicles at the early stage of endocytosis by inhibiting actin polymerisation. Further investigation to identify the endocytotic route of DMAEMA polyplexes was conducted applying clathrin- and caveolae- pathways inhibitors in cell transfection study. The results indicate that DMAEMA polyplexes were internalized primarily through clathrin-mediated pathway, with a minor fraction possibly entering cells via a caveolae-mediated pathway. © 2011 Informa UK, Ltd.en_HK
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1061186x.aspen_HK
dc.relation.ispartofJournal of Drug Targetingen_HK
dc.rightsJournal of Drug Targeting. Copyright © Informa Healthcare.-
dc.subjectActin filamentsen_HK
dc.subjectconfocal microscopyen_HK
dc.subjectendocytosisen_HK
dc.subjectgene deliveryen_HK
dc.subjectintracellular traffickingen_HK
dc.subjectmicrotubulesen_HK
dc.subjectplasmid DNAen_HK
dc.subjectpolymeren_HK
dc.subjecttransfectionen_HK
dc.subject.meshBiological Transport-
dc.subject.meshDNA - administration and dosage - chemistry-
dc.subject.meshGene Transfer Techniques-
dc.subject.meshMicrofilaments - metabolism-
dc.subject.meshMicrotubules - metabolism-
dc.titleThe involvement of microtubules and actin filaments in the intracellular transport of non-viral gene delivery systemen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1061-186X&volume=19&issue=1&spage=55&epage=66&date=2011&atitle=The+involvement+of+microtubules+and+actin+filaments+in+the+intracellular+transport+of+non-viral+gene+delivery+system-
dc.identifier.emailLam, JKW: jkwlam@hku.hken_HK
dc.identifier.authorityLam, JKW=rp01346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3109/10611861003733938en_HK
dc.identifier.pmid20353287-
dc.identifier.scopuseid_2-s2.0-78650046758en_HK
dc.identifier.hkuros188421en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650046758&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue1en_HK
dc.identifier.spage56en_HK
dc.identifier.epage66en_HK
dc.identifier.isiWOS:000285054900006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLam, JKW=8404243000en_HK
dc.identifier.scopusauthoridArmes, SP=7103125898en_HK
dc.identifier.scopusauthoridStolnik, S=35515143300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats