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Article: Cellular gene expression that correlates with EBER expression in epstein-barr virus-infected lymphoblastoid cell lines

TitleCellular gene expression that correlates with EBER expression in epstein-barr virus-infected lymphoblastoid cell lines
Authors
Issue Date2011
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2011, v. 85 n. 7, p. 3535-3545 How to Cite?
AbstractNovel Epstein-Barr Virus (EBV) strains with deletion of either EBER1 or EBER2 and corresponding revertant viruses were constructed and used to infect B lymphocytes to make lymphoblastoid cell lines (LCLs). The LCLs were used in microarray expression profiling to identify genes whose expression correlates with the presence of EBER1 or EBER2. Functions of regulated genes identified in the microarray analysis include membrane signaling, regulation of apoptosis, and the interferon/antiviral response. Although most emphasis has previously been given to EBER1 because it is more abundant than EBER2, the differences in cell gene expression were greater with EBER2 deletion. In this system, deletion of EBER1 or EBER2 had little effect on the EBV transformation frequency of primary B cells or the growth of the resulting LCLs. Using the recombinant viruses and novel EBER expression vectors, the nuclear redistribution of rpL22 protein by EBER1 in 293 cells was confirmed, but in LCLs almost all of the cells had a predominantly cytoplasmic expression of this ribosomal protein, which was not detectably changed by EBER1. The changes in LCL gene expression identified here will provide a basis for identifying the mechanisms of action of EBER RNAs. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/135328
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
European UnionLSHC-CT-2005-018704
Funding Information:

This study was supported in part by the Sixth Research Framework Programme of the European Union, project INCA (LSHC-CT-2005-018704).

References

 

DC FieldValueLanguage
dc.contributor.authorGregorovic, Gen_HK
dc.contributor.authorBosshard, Ren_HK
dc.contributor.authorKarstegl, CEen_HK
dc.contributor.authorWhite, REen_HK
dc.contributor.authorPattle, Sen_HK
dc.contributor.authorChiang, AKSen_HK
dc.contributor.authorDittrichBreiholz, Oen_HK
dc.contributor.authorKracht, Men_HK
dc.contributor.authorRuss, Ren_HK
dc.contributor.authorFarrell, PJen_HK
dc.date.accessioned2011-07-27T01:33:38Z-
dc.date.available2011-07-27T01:33:38Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Virology, 2011, v. 85 n. 7, p. 3535-3545en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135328-
dc.description.abstractNovel Epstein-Barr Virus (EBV) strains with deletion of either EBER1 or EBER2 and corresponding revertant viruses were constructed and used to infect B lymphocytes to make lymphoblastoid cell lines (LCLs). The LCLs were used in microarray expression profiling to identify genes whose expression correlates with the presence of EBER1 or EBER2. Functions of regulated genes identified in the microarray analysis include membrane signaling, regulation of apoptosis, and the interferon/antiviral response. Although most emphasis has previously been given to EBER1 because it is more abundant than EBER2, the differences in cell gene expression were greater with EBER2 deletion. In this system, deletion of EBER1 or EBER2 had little effect on the EBV transformation frequency of primary B cells or the growth of the resulting LCLs. Using the recombinant viruses and novel EBER expression vectors, the nuclear redistribution of rpL22 protein by EBER1 in 293 cells was confirmed, but in LCLs almost all of the cells had a predominantly cytoplasmic expression of this ribosomal protein, which was not detectably changed by EBER1. The changes in LCL gene expression identified here will provide a basis for identifying the mechanisms of action of EBER RNAs. Copyright © 2011, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_US
dc.subject.meshB-Lymphocytes - virology-
dc.subject.meshGene Expression Profiling-
dc.subject.meshHerpesvirus 4, Human - pathogenicity-
dc.subject.meshHost-Pathogen Interactions-
dc.subject.meshRNA, Viral - genetics - metabolism-
dc.titleCellular gene expression that correlates with EBER expression in epstein-barr virus-infected lymphoblastoid cell linesen_HK
dc.typeArticleen_HK
dc.identifier.emailChiang, AKS:chiangak@hkucc.hku.hken_HK
dc.identifier.authorityChiang, AKS=rp00403en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.02086-10en_HK
dc.identifier.pmid21248031-
dc.identifier.pmcidPMC3067860-
dc.identifier.scopuseid_2-s2.0-79952613155en_HK
dc.identifier.hkuros186804en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952613155&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume85en_HK
dc.identifier.issue7en_HK
dc.identifier.spage3535en_HK
dc.identifier.epage3545en_HK
dc.identifier.isiWOS:000288373000046-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGregorovic, G=8162224400en_HK
dc.identifier.scopusauthoridBosshard, R=35081802600en_HK
dc.identifier.scopusauthoridKarstegl, CE=6506183764en_HK
dc.identifier.scopusauthoridWhite, RE=7501422734en_HK
dc.identifier.scopusauthoridPattle, S=37023222100en_HK
dc.identifier.scopusauthoridChiang, AKS=7101623534en_HK
dc.identifier.scopusauthoridDittrichBreiholz, O=8206385800en_HK
dc.identifier.scopusauthoridKracht, M=7005168174en_HK
dc.identifier.scopusauthoridRuss, R=24475188900en_HK
dc.identifier.scopusauthoridFarrell, PJ=7201756081en_HK
dc.identifier.citeulike8685088-

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