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Article: Micro-CT-based bone ceramic scaffolding and its performance after seeding with mesenchymal stem cells for repair of load-bearing bone defect in canine femoral head

TitleMicro-CT-based bone ceramic scaffolding and its performance after seeding with mesenchymal stem cells for repair of load-bearing bone defect in canine femoral head
Authors
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/
Citation
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2011, v. 96 n. 2, p. 316-325 How to Cite?
AbstractOsteonecrosis of the femoral head is a debilitating and painful orthopedic condition characterized by joint collapse. Salvage of the femoral head is highly desirable to preserve the contour and mechanical properties and prevent joint collapse. This study aimed to develop a new tissue-engineering approach for treatment of large bone defect in femoral head, that is, after osteonecrosis. The biphasic calcium phosphate (BCP) ceramic scaffolds were fabricated by a 3D gel-lamination technique based on micro-computed tomography (micro-CT) images of the cancellous bone microarchitecture of femoral heads. After seeding with autologous bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, the cell-scaffold composite was implanted into a bone defect surgically induced in canine femoral head via trapdoor procedure, which was a common procedure for treatment of osteonecrosis. A total of 24 adult dogs were randomly divided into three groups (n = 8 each) for implantation of the BCP scaffold with or without with BMSCs, and also the autologous bone chips for comparisons. All animals were sacrificed at 30 weeks postoperatively and processed for radiological and histological evaluations. The contour of the femoral head was well preserved with implantation of BCP scaffolds with or without BMSCs, whereas joint collapse was found after treatment with autologous bone chips. The osteointegration and new bone formation was significantly greater with BCP scaffold implantation with than without BMSC seeding and showed greater strength and compressive modulus in the repair site. Micro-CT-based bone ceramic scaffolds seeding with BMSC might be a promising way to repair bone defects in the femoral head.
Persistent Identifierhttp://hdl.handle.net/10722/135301
ISSN
2015 Impact Factor: 2.881
2015 SCImago Journal Rankings: 0.784
ISI Accession Number ID
Funding AgencyGrant Number
Nature Natural Science Foundation of China30930092
Medical Health Research Foundation Project of the Chinese PLA06Z057
Funding Information:

Contract grant sponsor: The Nature Natural Science Foundation of China; contract grant number: 30930092

 

DC FieldValueLanguage
dc.contributor.authorPeng, Jen_US
dc.contributor.authorWen, Cen_US
dc.contributor.authorWang, Aen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorXu, Wen_US
dc.contributor.authorZhao, Ben_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorLu, Sen_US
dc.contributor.authorQin, Len_US
dc.contributor.authorGuo, Qen_US
dc.contributor.authorDong, Len_US
dc.contributor.authorTian, Jen_US
dc.date.accessioned2011-07-27T01:31:46Z-
dc.date.available2011-07-27T01:31:46Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2011, v. 96 n. 2, p. 316-325en_US
dc.identifier.issn1552-4973en_US
dc.identifier.urihttp://hdl.handle.net/10722/135301-
dc.description.abstractOsteonecrosis of the femoral head is a debilitating and painful orthopedic condition characterized by joint collapse. Salvage of the femoral head is highly desirable to preserve the contour and mechanical properties and prevent joint collapse. This study aimed to develop a new tissue-engineering approach for treatment of large bone defect in femoral head, that is, after osteonecrosis. The biphasic calcium phosphate (BCP) ceramic scaffolds were fabricated by a 3D gel-lamination technique based on micro-computed tomography (micro-CT) images of the cancellous bone microarchitecture of femoral heads. After seeding with autologous bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, the cell-scaffold composite was implanted into a bone defect surgically induced in canine femoral head via trapdoor procedure, which was a common procedure for treatment of osteonecrosis. A total of 24 adult dogs were randomly divided into three groups (n = 8 each) for implantation of the BCP scaffold with or without with BMSCs, and also the autologous bone chips for comparisons. All animals were sacrificed at 30 weeks postoperatively and processed for radiological and histological evaluations. The contour of the femoral head was well preserved with implantation of BCP scaffolds with or without BMSCs, whereas joint collapse was found after treatment with autologous bone chips. The osteointegration and new bone formation was significantly greater with BCP scaffold implantation with than without BMSC seeding and showed greater strength and compressive modulus in the repair site. Micro-CT-based bone ceramic scaffolds seeding with BMSC might be a promising way to repair bone defects in the femoral head.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0021-9304:1/-
dc.relation.ispartofJournal of Biomedical Materials Research Part B: Applied Biomaterialsen_US
dc.rightsJournal of Biomedical Materials Research Part B: Applied Biomaterials. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshBone and Bones - injuries-
dc.subject.meshFemur Head - injuries-
dc.subject.meshFemur Head Necrosis - therapy-
dc.subject.meshMesenchymal Stem Cells - cytology-
dc.subject.meshTissue Scaffolds - chemistry-
dc.titleMicro-CT-based bone ceramic scaffolding and its performance after seeding with mesenchymal stem cells for repair of load-bearing bone defect in canine femoral headen_US
dc.typeArticleen_US
dc.identifier.emailWen, C: paulwen@hku.hken_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jbm.b.31770-
dc.identifier.pmid21210512-
dc.identifier.scopuseid_2-s2.0-78651080413-
dc.identifier.hkuros188830en_US
dc.identifier.volume96en_US
dc.identifier.issue2en_US
dc.identifier.spage316en_US
dc.identifier.epage325en_US
dc.identifier.isiWOS:000286169600016-
dc.publisher.placeUnited States-

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