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Article: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells
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TitleIrradiation induces bone injury by damaging bone marrow microenvironment for stem cells
 
AuthorsCao, X3
Wu, X3 2
Frassica, D3
Yu, B3
Pang, L3 2
Xian, L3
Wan, M3
Lei, W3
Armour, M3
Tryggestad, E3
Wong, J3
Wen, CY1
Lu, WW1
Frassica, FJ3
 
KeywordsAngiography
CFU-fibroblast
Differentiation
Repopulation
Self-renewal
 
Issue Date2011
 
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
 
CitationProceedings Of The National Academy Of Sciences Of The United States Of America, 2011, v. 108 n. 4, p. 1609-1614 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1015350108
 
AbstractRadiation therapy can result in bone injury with the development of fractures and often can lead to delayed and nonunion of bone. There is no prevention or treatment for irradiation-induced bone injury. We irradiated the distal half of the mouse left femur to study the mechanism of irradiation-induced bone injury and found that no mesenchymal stem cells (MSCs) were detected in irradiated distal femora or nonirradiated proximal femora. The MSCs in the circulation doubled at 1 week and increased fourfold after 4 wk of irradiation. The number of MSCs in the proximal femur quickly recovered, but no recovery was observed in the distal femur. The levels of free radicals were increased threefold at 1 wk and remained at this high level for 4 wk in distal femora, whereas the levels were increased at 1 wk and returned to the basal level at 4 wk in nonirradiated proximal femur. Free radicals diffuse ipsilaterally to the proximal femur through bone medullary canal. The blood vessels in the distal femora were destroyed in angiographic images, but not in the proximal femora. The osteoclasts and osteoblasts were decreased in the distal femora after irradiation, but no changes were observed in the proximal femora. The total bone volumes were not affected in proximal and distal femora. Our data indicate that irradiation produces free radicals that adversely affect the survival of MSCs in both distal and proximal femora. Irradiation injury to the vasculatures and the microenvironment affect the niches for stem cells during the recovery period.
 
Descriptioneid_2-s2.0-79955043850 = Erratum: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells (Proceedings of the National Academy of Sciences of the United States of America (2011) 108 (1609-1614) DOI:10.1073/pnas. 1015350108)
 
ISSN0027-8424
2012 Impact Factor: 9.737
2012 SCImago Journal Rankings: 5.473
 
DOIhttp://dx.doi.org/10.1073/pnas.1015350108
 
PubMed Central IDPMC3029740
 
ISI Accession Number IDWOS:000286594800074
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCao, X
 
dc.contributor.authorWu, X
 
dc.contributor.authorFrassica, D
 
dc.contributor.authorYu, B
 
dc.contributor.authorPang, L
 
dc.contributor.authorXian, L
 
dc.contributor.authorWan, M
 
dc.contributor.authorLei, W
 
dc.contributor.authorArmour, M
 
dc.contributor.authorTryggestad, E
 
dc.contributor.authorWong, J
 
dc.contributor.authorWen, CY
 
dc.contributor.authorLu, WW
 
dc.contributor.authorFrassica, FJ
 
dc.date.accessioned2011-07-27T01:31:41Z
 
dc.date.available2011-07-27T01:31:41Z
 
dc.date.issued2011
 
dc.description.abstractRadiation therapy can result in bone injury with the development of fractures and often can lead to delayed and nonunion of bone. There is no prevention or treatment for irradiation-induced bone injury. We irradiated the distal half of the mouse left femur to study the mechanism of irradiation-induced bone injury and found that no mesenchymal stem cells (MSCs) were detected in irradiated distal femora or nonirradiated proximal femora. The MSCs in the circulation doubled at 1 week and increased fourfold after 4 wk of irradiation. The number of MSCs in the proximal femur quickly recovered, but no recovery was observed in the distal femur. The levels of free radicals were increased threefold at 1 wk and remained at this high level for 4 wk in distal femora, whereas the levels were increased at 1 wk and returned to the basal level at 4 wk in nonirradiated proximal femur. Free radicals diffuse ipsilaterally to the proximal femur through bone medullary canal. The blood vessels in the distal femora were destroyed in angiographic images, but not in the proximal femora. The osteoclasts and osteoblasts were decreased in the distal femora after irradiation, but no changes were observed in the proximal femora. The total bone volumes were not affected in proximal and distal femora. Our data indicate that irradiation produces free radicals that adversely affect the survival of MSCs in both distal and proximal femora. Irradiation injury to the vasculatures and the microenvironment affect the niches for stem cells during the recovery period.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptioneid_2-s2.0-79955043850 = Erratum: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells (Proceedings of the National Academy of Sciences of the United States of America (2011) 108 (1609-1614) DOI:10.1073/pnas. 1015350108)
 
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 2011, v. 108 n. 4, p. 1609-1614 [How to Cite?]
DOI: http://dx.doi.org/10.1073/pnas.1015350108
 
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1015350108
 
dc.identifier.eissn1091-6490
 
dc.identifier.epage1614
 
dc.identifier.hkuros188827
 
dc.identifier.isiWOS:000286594800074
 
dc.identifier.issn0027-8424
2012 Impact Factor: 9.737
2012 SCImago Journal Rankings: 5.473
 
dc.identifier.issue4
 
dc.identifier.pmcidPMC3029740
 
dc.identifier.pmid21220327
 
dc.identifier.scopuseid_2-s2.0-79952166223
 
dc.identifier.spage1609
 
dc.identifier.urihttp://hdl.handle.net/10722/135298
 
dc.identifier.volume108
 
dc.languageeng
 
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBone Marrow - pathology - radiation effects
 
dc.subject.meshBone Marrow Cells - metabolism - pathology - radiation effects
 
dc.subject.meshFemur - metabolism - pathology - radiation effects
 
dc.subject.meshMembrane Proteins - metabolism
 
dc.subject.meshMesenchymal Stem Cells - metabolism - pathology - radiation effects
 
dc.subjectAngiography
 
dc.subjectCFU-fibroblast
 
dc.subjectDifferentiation
 
dc.subjectRepopulation
 
dc.subjectSelf-renewal
 
dc.titleIrradiation induces bone injury by damaging bone marrow microenvironment for stem cells
 
dc.typeArticle
 
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<contributor.author>Yu, B</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Shihezi University
  3. The Johns Hopkins School of Medicine