Article: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells

File Download

re01.htm

Links for fulltext
(May Require Subscription)

Publisher Website: 10.1073/pnas.1015350108
Scopus: eid_2-s2.0-79952166223
PMID: 21220327

Supplementary

Citations:
- Scopus:
- Web of Science:
- PubMed Central:
Item Statistics (The Hub)
Appears in Collections:
- Orthopaedics & Traumatology: Journal/Magazine Articles

Title	Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells
Authors	Cao, X3 Wu, X2 3 Frassica, D3 Yu, B3 Pang, L2 3 Xian, L3 Wan, M3 Lei, W3 Armour, M3 Tryggestad, E3 Wong, J3 Wen, CY1 Lu, WW1 Frassica, FJ3
Keywords	Angiography CFU-fibroblast Differentiation Repopulation Self-renewal
Issue Date	2011
Publisher	National Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation	Proceedings Of The National Academy Of Sciences Of The United States Of America, 2011, v. 108 n. 4, p. 1609-1614 [How to Cite?] DOI: http://dx.doi.org/10.1073/pnas.1015350108
Abstract	Radiation therapy can result in bone injury with the development of fractures and often can lead to delayed and nonunion of bone. There is no prevention or treatment for irradiation-induced bone injury. We irradiated the distal half of the mouse left femur to study the mechanism of irradiation-induced bone injury and found that no mesenchymal stem cells (MSCs) were detected in irradiated distal femora or nonirradiated proximal femora. The MSCs in the circulation doubled at 1 week and increased fourfold after 4 wk of irradiation. The number of MSCs in the proximal femur quickly recovered, but no recovery was observed in the distal femur. The levels of free radicals were increased threefold at 1 wk and remained at this high level for 4 wk in distal femora, whereas the levels were increased at 1 wk and returned to the basal level at 4 wk in nonirradiated proximal femur. Free radicals diffuse ipsilaterally to the proximal femur through bone medullary canal. The blood vessels in the distal femora were destroyed in angiographic images, but not in the proximal femora. The osteoclasts and osteoblasts were decreased in the distal femora after irradiation, but no changes were observed in the proximal femora. The total bone volumes were not affected in proximal and distal femora. Our data indicate that irradiation produces free radicals that adversely affect the survival of MSCs in both distal and proximal femora. Irradiation injury to the vasculatures and the microenvironment affect the niches for stem cells during the recovery period.
Description	eid_2-s2.0-79955043850 = Erratum: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells (Proceedings of the National Academy of Sciences of the United States of America (2011) 108 (1609-1614) DOI:10.1073/pnas. 1015350108)
ISSN	0027-8424 2011 Impact Factor: 9.681 2011 SCImago Journal Rankings: 1.754
DOI	http://dx.doi.org/10.1073/pnas.1015350108
ISI Accession Number ID	WOS:000286594800074
PubMed Central ID	PMC3029740
References	References in Scopus

DC Field	Value
dc.contributor.author	Cao, X
dc.contributor.author	Wu, X
dc.contributor.author	Frassica, D
dc.contributor.author	Yu, B
dc.contributor.author	Pang, L
dc.contributor.author	Xian, L
dc.contributor.author	Wan, M
dc.contributor.author	Lei, W
dc.contributor.author	Armour, M
dc.contributor.author	Tryggestad, E
dc.contributor.author	Wong, J
dc.contributor.author	Wen, CY
dc.contributor.author	Lu, WW
dc.contributor.author	Frassica, FJ
dc.date.accessioned	2011-07-27T01:31:41Z
dc.date.available	2011-07-27T01:31:41Z
dc.date.issued	2011
dc.description.abstract	Radiation therapy can result in bone injury with the development of fractures and often can lead to delayed and nonunion of bone. There is no prevention or treatment for irradiation-induced bone injury. We irradiated the distal half of the mouse left femur to study the mechanism of irradiation-induced bone injury and found that no mesenchymal stem cells (MSCs) were detected in irradiated distal femora or nonirradiated proximal femora. The MSCs in the circulation doubled at 1 week and increased fourfold after 4 wk of irradiation. The number of MSCs in the proximal femur quickly recovered, but no recovery was observed in the distal femur. The levels of free radicals were increased threefold at 1 wk and remained at this high level for 4 wk in distal femora, whereas the levels were increased at 1 wk and returned to the basal level at 4 wk in nonirradiated proximal femur. Free radicals diffuse ipsilaterally to the proximal femur through bone medullary canal. The blood vessels in the distal femora were destroyed in angiographic images, but not in the proximal femora. The osteoclasts and osteoblasts were decreased in the distal femora after irradiation, but no changes were observed in the proximal femora. The total bone volumes were not affected in proximal and distal femora. Our data indicate that irradiation produces free radicals that adversely affect the survival of MSCs in both distal and proximal femora. Irradiation injury to the vasculatures and the microenvironment affect the niches for stem cells during the recovery period.
dc.description.nature	link_to_OA_fulltext
dc.description	eid_2-s2.0-79955043850 = Erratum: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells (Proceedings of the National Academy of Sciences of the United States of America (2011) 108 (1609-1614) DOI:10.1073/pnas. 1015350108)
dc.identifier.citation	Proceedings Of The National Academy Of Sciences Of The United States Of America, 2011, v. 108 n. 4, p. 1609-1614 [How to Cite?] DOI: http://dx.doi.org/10.1073/pnas.1015350108
dc.identifier.doi	http://dx.doi.org/10.1073/pnas.1015350108
dc.identifier.epage	1614
dc.identifier.hkuros	188827
dc.identifier.isi	WOS:000286594800074
dc.identifier.issn	0027-8424 2011 Impact Factor: 9.681 2011 SCImago Journal Rankings: 1.754
dc.identifier.issue	4
dc.identifier.pmcid	PMC3029740
dc.identifier.pmid	21220327
dc.identifier.scopus	eid_2-s2.0-79952166223
dc.identifier.spage	1609
dc.identifier.uri	http://hdl.handle.net/10722/135298
dc.identifier.volume	108
dc.language	eng
dc.publisher	National Academy of Sciences. The Journal's web site is located at http://www.pnas.org
dc.publisher.place	United States
dc.relation.ispartof	Proceedings of the National Academy of Sciences of the United States of America
dc.relation.references	References in Scopus
dc.subject.mesh	Bone Marrow - pathology - radiation effects
dc.subject.mesh	Bone Marrow Cells - metabolism - pathology - radiation effects
dc.subject.mesh	Femur - metabolism - pathology - radiation effects
dc.subject.mesh	Membrane Proteins - metabolism
dc.subject.mesh	Mesenchymal Stem Cells - metabolism - pathology - radiation effects
dc.subject	Angiography
dc.subject	CFU-fibroblast
dc.subject	Differentiation
dc.subject	Repopulation
dc.subject	Self-renewal
dc.title	Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells
dc.type	Article

<?xml encoding="utf-8" version="1.0"?><item><contributor.author>Cao, X</contributor.author><contributor.author>Wu, X</contributor.author><contributor.author>Frassica, D</contributor.author><contributor.author>Yu, B</contributor.author><contributor.author>Pang, L</contributor.author><contributor.author>Xian, L</contributor.author><contributor.author>Wan, M</contributor.author><contributor.author>Lei, W</contributor.author><contributor.author>Armour, M</contributor.author><contributor.author>Tryggestad, E</contributor.author><contributor.author>Wong, J</contributor.author><contributor.author>Wen, CY</contributor.author><contributor.author>Lu, WW</contributor.author><contributor.author>Frassica, FJ</contributor.author><date.accessioned>2011-07-27T01:31:41Z</date.accessioned><date.available>2011-07-27T01:31:41Z</date.available><date.issued>2011</date.issued><identifier.citation>Proceedings Of The National Academy Of Sciences Of The United States Of America, 2011, v. 108 n. 4, p. 1609-1614</identifier.citation><identifier.issn>0027-8424</identifier.issn><identifier.uri>http://hdl.handle.net/10722/135298</identifier.uri><description>eid_2-s2.0-79955043850 = Erratum: Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells (Proceedings of the National Academy of Sciences of the United States of America (2011) 108 (1609-1614) DOI:10.1073/pnas. 1015350108)</description><description.abstract>Radiation therapy can result in bone injury with the development of fractures and often can lead to delayed and nonunion of bone. There is no prevention or treatment for irradiation-induced bone injury. We irradiated the distal half of the mouse left femur to study the mechanism of irradiation-induced bone injury and found that no mesenchymal stem cells (MSCs) were detected in irradiated distal femora or nonirradiated proximal femora. The MSCs in the circulation doubled at 1 week and increased fourfold after 4 wk of irradiation. The number of MSCs in the proximal femur quickly recovered, but no recovery was observed in the distal femur. The levels of free radicals were increased threefold at 1 wk and remained at this high level for 4 wk in distal femora, whereas the levels were increased at 1 wk and returned to the basal level at 4 wk in nonirradiated proximal femur. Free radicals diffuse ipsilaterally to the proximal femur through bone medullary canal. The blood vessels in the distal femora were destroyed in angiographic images, but not in the proximal femora. The osteoclasts and osteoblasts were decreased in the distal femora after irradiation, but no changes were observed in the proximal femora. The total bone volumes were not affected in proximal and distal femora. Our data indicate that irradiation produces free radicals that adversely affect the survival of MSCs in both distal and proximal femora. Irradiation injury to the vasculatures and the microenvironment affect the niches for stem cells during the recovery period.</description.abstract><language>eng</language><publisher>National Academy of Sciences. The Journal&apos;s web site is located at http://www.pnas.org</publisher><relation.ispartof>Proceedings of the National Academy of Sciences of the United States of America</relation.ispartof><subject>Angiography</subject><subject>CFU-fibroblast</subject><subject>Differentiation</subject><subject>Repopulation</subject><subject>Self-renewal</subject><subject.mesh>Bone Marrow - pathology - radiation effects</subject.mesh><subject.mesh>Bone Marrow Cells - metabolism - pathology - radiation effects</subject.mesh><subject.mesh>Femur - metabolism - pathology - radiation effects</subject.mesh><subject.mesh>Membrane Proteins - metabolism</subject.mesh><subject.mesh>Mesenchymal Stem Cells - metabolism - pathology - radiation effects</subject.mesh><title>Irradiation induces bone injury by damaging bone marrow microenvironment for stem cells</title><type>Article</type><description.nature>link_to_OA_fulltext</description.nature><identifier.doi>10.1073/pnas.1015350108</identifier.doi><identifier.pmid>21220327</identifier.pmid><identifier.pmcid>PMC3029740</identifier.pmcid><identifier.scopus>eid_2-s2.0-79952166223</identifier.scopus><identifier.hkuros>188827</identifier.hkuros><relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-79952166223&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references><identifier.volume>108</identifier.volume><identifier.issue>4</identifier.issue><identifier.spage>1609</identifier.spage><identifier.epage>1614</identifier.epage><identifier.isi>WOS:000286594800074</identifier.isi><publisher.place>United States</publisher.place><bitstream.url>http://hub.hku.hk/bitstream/10722/135298/1/re01.htm</bitstream.url></item>

Author Affiliations

The University of Hong Kong
Shihezi University
The Johns Hopkins School of Medicine