Article: Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup

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TitleCoexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
AuthorsLau, SKP2
Poon, RWS2
Wong, BHL2
Wang, M1
Huang, Y2
Xu, H1
Guo, R1
Li, KSM2
Gao, K1
Chan, KH2
Zheng, BJ2
Woo, PCY2
Yuen, KY2
Issue Date2010
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
CitationJournal Of Virology, 2010, v. 84 n. 21, p. 11385-11394 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.01121-10
AbstractRousettus bat coronavirus HKU9 (Ro-BatCoV HKU9), a recently identified coronavirus of novel Betacoronavirus subgroup D, from Leschenault's rousette, was previously found to display marked sequence polymorphism among genomes of four strains. Among 10 bats with complete RNA-dependent RNA polymerase (RdRp), spike (S), and nucleocapsid (N) genes sequenced, three and two sequence clades for all three genes were codetected in two and five bats, respectively, suggesting the coexistence of two or three distinct genotypes of Ro-BatCoV HKU9 in the same bat. Complete genome sequencing of the distinct genotypes from two bats, using degenerate/genome-specific primers with overlapping sequences confirmed by specific PCR, supported the coexistence of at least two distinct genomes in each bat. Recombination analysis using eight Ro-BatCoV HKU9 genomes showed possible recombination events between strains from different bat individuals, which may have allowed for the generation of different genotypes. Western blot assays using recombinant N proteins of Ro-BatCoV HKU9, Betacoronavirus subgroup A (HCoV-HKU1), subgroup B (SARSr-Rh-BatCoV), and subgroup C (Ty-BatCoV HKU4 and Pi-BatCoV HKU5) coronaviruses were subgroup specific, supporting their classification as separate subgroups under Betacoronavirus. Antibodies were detected in 75 (43%) of 175 and 224 (64%) of 350 tested serum samples from Leschenault's rousette bats by Ro-BatCoV HKU9 N-protein-based Western blot and enzyme immunoassays, respectively. This is the first report describing coinfection of different coronavirus genotypes in bats and coronavirus genotypes of diverse nucleotide variation in the same host. Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault's rousette. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
ISSN0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
DOIhttp://dx.doi.org/10.1128/JVI.01121-10
ISI Accession Number IDWOS:000282643400042
Funding AgencyGrant Number
Research Grant Council
University Development Fund
University of Hong Kong
Funding Information:

This work is partly supported by the Research Grant Council Grant and University Development Fund, the University of Hong Kong; The Tung Wah Group of Hospitals Fund for Research in Infectious Diseases; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; the Providence Foundation Limited in memory of the late Lui Hac Minh; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

PubMed Central IDPMC2953156
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLau, SKP
dc.contributor.authorPoon, RWS
dc.contributor.authorWong, BHL
dc.contributor.authorWang, M
dc.contributor.authorHuang, Y
dc.contributor.authorXu, H
dc.contributor.authorGuo, R
dc.contributor.authorLi, KSM
dc.contributor.authorGao, K
dc.contributor.authorChan, KH
dc.contributor.authorZheng, BJ
dc.contributor.authorWoo, PCY
dc.contributor.authorYuen, KY
dc.date.accessioned2011-07-27T01:31:09Z
dc.date.available2011-07-27T01:31:09Z
dc.date.issued2010
dc.description.abstractRousettus bat coronavirus HKU9 (Ro-BatCoV HKU9), a recently identified coronavirus of novel Betacoronavirus subgroup D, from Leschenault's rousette, was previously found to display marked sequence polymorphism among genomes of four strains. Among 10 bats with complete RNA-dependent RNA polymerase (RdRp), spike (S), and nucleocapsid (N) genes sequenced, three and two sequence clades for all three genes were codetected in two and five bats, respectively, suggesting the coexistence of two or three distinct genotypes of Ro-BatCoV HKU9 in the same bat. Complete genome sequencing of the distinct genotypes from two bats, using degenerate/genome-specific primers with overlapping sequences confirmed by specific PCR, supported the coexistence of at least two distinct genomes in each bat. Recombination analysis using eight Ro-BatCoV HKU9 genomes showed possible recombination events between strains from different bat individuals, which may have allowed for the generation of different genotypes. Western blot assays using recombinant N proteins of Ro-BatCoV HKU9, Betacoronavirus subgroup A (HCoV-HKU1), subgroup B (SARSr-Rh-BatCoV), and subgroup C (Ty-BatCoV HKU4 and Pi-BatCoV HKU5) coronaviruses were subgroup specific, supporting their classification as separate subgroups under Betacoronavirus. Antibodies were detected in 75 (43%) of 175 and 224 (64%) of 350 tested serum samples from Leschenault's rousette bats by Ro-BatCoV HKU9 N-protein-based Western blot and enzyme immunoassays, respectively. This is the first report describing coinfection of different coronavirus genotypes in bats and coronavirus genotypes of diverse nucleotide variation in the same host. Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault's rousette. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationJournal Of Virology, 2010, v. 84 n. 21, p. 11385-11394 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.01121-10
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.01121-10
dc.identifier.epage11394
dc.identifier.hkuros188637
dc.identifier.isiWOS:000282643400042
Funding AgencyGrant Number
Research Grant Council
University Development Fund
University of Hong Kong
Funding Information:

This work is partly supported by the Research Grant Council Grant and University Development Fund, the University of Hong Kong; The Tung Wah Group of Hospitals Fund for Research in Infectious Diseases; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; the Providence Foundation Limited in memory of the late Lui Hac Minh; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

dc.identifier.issn0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
dc.identifier.issue21
dc.identifier.pmcidPMC2953156
dc.identifier.pmid20702646
dc.identifier.scopuseid_2-s2.0-77957962420
dc.identifier.spage11385
dc.identifier.urihttp://hdl.handle.net/10722/135279
dc.identifier.volume84
dc.languageeng
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Virology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
dc.subject.meshAntibodies, Viral - blood
dc.subject.meshChiroptera - virology
dc.subject.meshComorbidity
dc.subject.meshCoronavirus - classification - genetics
dc.subject.meshPolymorphism, Genetic
dc.titleCoexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
dc.typeArticle
Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. The University of Hong Kong