File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
  • Basic View
  • Metadata View
  • XML View
TitleCoexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
 
AuthorsLau, SKP2 2 2 2
Poon, RWS2
Wong, BHL2
Wang, M1
Huang, Y2
Xu, H1
Guo, R1
Li, KSM2
Gao, K1
Chan, KH2
Zheng, BJ2 2 2 2
Woo, PCY2 2 2 2
Yuen, KY2 2 2 2
 
Issue Date2010
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
CitationJournal Of Virology, 2010, v. 84 n. 21, p. 11385-11394 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.01121-10
 
AbstractRousettus bat coronavirus HKU9 (Ro-BatCoV HKU9), a recently identified coronavirus of novel Betacoronavirus subgroup D, from Leschenault's rousette, was previously found to display marked sequence polymorphism among genomes of four strains. Among 10 bats with complete RNA-dependent RNA polymerase (RdRp), spike (S), and nucleocapsid (N) genes sequenced, three and two sequence clades for all three genes were codetected in two and five bats, respectively, suggesting the coexistence of two or three distinct genotypes of Ro-BatCoV HKU9 in the same bat. Complete genome sequencing of the distinct genotypes from two bats, using degenerate/genome-specific primers with overlapping sequences confirmed by specific PCR, supported the coexistence of at least two distinct genomes in each bat. Recombination analysis using eight Ro-BatCoV HKU9 genomes showed possible recombination events between strains from different bat individuals, which may have allowed for the generation of different genotypes. Western blot assays using recombinant N proteins of Ro-BatCoV HKU9, Betacoronavirus subgroup A (HCoV-HKU1), subgroup B (SARSr-Rh-BatCoV), and subgroup C (Ty-BatCoV HKU4 and Pi-BatCoV HKU5) coronaviruses were subgroup specific, supporting their classification as separate subgroups under Betacoronavirus. Antibodies were detected in 75 (43%) of 175 and 224 (64%) of 350 tested serum samples from Leschenault's rousette bats by Ro-BatCoV HKU9 N-protein-based Western blot and enzyme immunoassays, respectively. This is the first report describing coinfection of different coronavirus genotypes in bats and coronavirus genotypes of diverse nucleotide variation in the same host. Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault's rousette. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
 
ISSN0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
DOIhttp://dx.doi.org/10.1128/JVI.01121-10
 
PubMed Central IDPMC2953156
 
ISI Accession Number IDWOS:000282643400042
Funding AgencyGrant Number
Research Grant Council
University Development Fund
University of Hong Kong
Funding Information:

This work is partly supported by the Research Grant Council Grant and University Development Fund, the University of Hong Kong; The Tung Wah Group of Hospitals Fund for Research in Infectious Diseases; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; the Providence Foundation Limited in memory of the late Lui Hac Minh; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLau, SKP
 
dc.contributor.authorPoon, RWS
 
dc.contributor.authorWong, BHL
 
dc.contributor.authorWang, M
 
dc.contributor.authorHuang, Y
 
dc.contributor.authorXu, H
 
dc.contributor.authorGuo, R
 
dc.contributor.authorLi, KSM
 
dc.contributor.authorGao, K
 
dc.contributor.authorChan, KH
 
dc.contributor.authorZheng, BJ
 
dc.contributor.authorWoo, PCY
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2011-07-27T01:31:09Z
 
dc.date.available2011-07-27T01:31:09Z
 
dc.date.issued2010
 
dc.description.abstractRousettus bat coronavirus HKU9 (Ro-BatCoV HKU9), a recently identified coronavirus of novel Betacoronavirus subgroup D, from Leschenault's rousette, was previously found to display marked sequence polymorphism among genomes of four strains. Among 10 bats with complete RNA-dependent RNA polymerase (RdRp), spike (S), and nucleocapsid (N) genes sequenced, three and two sequence clades for all three genes were codetected in two and five bats, respectively, suggesting the coexistence of two or three distinct genotypes of Ro-BatCoV HKU9 in the same bat. Complete genome sequencing of the distinct genotypes from two bats, using degenerate/genome-specific primers with overlapping sequences confirmed by specific PCR, supported the coexistence of at least two distinct genomes in each bat. Recombination analysis using eight Ro-BatCoV HKU9 genomes showed possible recombination events between strains from different bat individuals, which may have allowed for the generation of different genotypes. Western blot assays using recombinant N proteins of Ro-BatCoV HKU9, Betacoronavirus subgroup A (HCoV-HKU1), subgroup B (SARSr-Rh-BatCoV), and subgroup C (Ty-BatCoV HKU4 and Pi-BatCoV HKU5) coronaviruses were subgroup specific, supporting their classification as separate subgroups under Betacoronavirus. Antibodies were detected in 75 (43%) of 175 and 224 (64%) of 350 tested serum samples from Leschenault's rousette bats by Ro-BatCoV HKU9 N-protein-based Western blot and enzyme immunoassays, respectively. This is the first report describing coinfection of different coronavirus genotypes in bats and coronavirus genotypes of diverse nucleotide variation in the same host. Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault's rousette. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Virology, 2010, v. 84 n. 21, p. 11385-11394 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.01121-10
 
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.01121-10
 
dc.identifier.epage11394
 
dc.identifier.hkuros188637
 
dc.identifier.isiWOS:000282643400042
Funding AgencyGrant Number
Research Grant Council
University Development Fund
University of Hong Kong
Funding Information:

This work is partly supported by the Research Grant Council Grant and University Development Fund, the University of Hong Kong; The Tung Wah Group of Hospitals Fund for Research in Infectious Diseases; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; the Providence Foundation Limited in memory of the late Lui Hac Minh; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

 
dc.identifier.issn0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
dc.identifier.issue21
 
dc.identifier.pmcidPMC2953156
 
dc.identifier.pmid20702646
 
dc.identifier.scopuseid_2-s2.0-77957962420
 
dc.identifier.spage11385
 
dc.identifier.urihttp://hdl.handle.net/10722/135279
 
dc.identifier.volume84
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
 
dc.subject.meshAntibodies, Viral - blood
 
dc.subject.meshChiroptera - virology
 
dc.subject.meshComorbidity
 
dc.subject.meshCoronavirus - classification - genetics
 
dc.subject.meshPolymorphism, Genetic
 
dc.titleCoexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Lau, SKP</contributor.author>
<contributor.author>Poon, RWS</contributor.author>
<contributor.author>Wong, BHL</contributor.author>
<contributor.author>Wang, M</contributor.author>
<contributor.author>Huang, Y</contributor.author>
<contributor.author>Xu, H</contributor.author>
<contributor.author>Guo, R</contributor.author>
<contributor.author>Li, KSM</contributor.author>
<contributor.author>Gao, K</contributor.author>
<contributor.author>Chan, KH</contributor.author>
<contributor.author>Zheng, BJ</contributor.author>
<contributor.author>Woo, PCY</contributor.author>
<contributor.author>Yuen, KY</contributor.author>
<date.accessioned>2011-07-27T01:31:09Z</date.accessioned>
<date.available>2011-07-27T01:31:09Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>Journal Of Virology, 2010, v. 84 n. 21, p. 11385-11394</identifier.citation>
<identifier.issn>0022-538X</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/135279</identifier.uri>
<description.abstract>Rousettus bat coronavirus HKU9 (Ro-BatCoV HKU9), a recently identified coronavirus of novel Betacoronavirus subgroup D, from Leschenault&apos;s rousette, was previously found to display marked sequence polymorphism among genomes of four strains. Among 10 bats with complete RNA-dependent RNA polymerase (RdRp), spike (S), and nucleocapsid (N) genes sequenced, three and two sequence clades for all three genes were codetected in two and five bats, respectively, suggesting the coexistence of two or three distinct genotypes of Ro-BatCoV HKU9 in the same bat. Complete genome sequencing of the distinct genotypes from two bats, using degenerate/genome-specific primers with overlapping sequences confirmed by specific PCR, supported the coexistence of at least two distinct genomes in each bat. Recombination analysis using eight Ro-BatCoV HKU9 genomes showed possible recombination events between strains from different bat individuals, which may have allowed for the generation of different genotypes. Western blot assays using recombinant N proteins of Ro-BatCoV HKU9, Betacoronavirus subgroup A (HCoV-HKU1), subgroup B (SARSr-Rh-BatCoV), and subgroup C (Ty-BatCoV HKU4 and Pi-BatCoV HKU5) coronaviruses were subgroup specific, supporting their classification as separate subgroups under Betacoronavirus. Antibodies were detected in 75 (43%) of 175 and 224 (64%) of 350 tested serum samples from Leschenault&apos;s rousette bats by Ro-BatCoV HKU9 N-protein-based Western blot and enzyme immunoassays, respectively. This is the first report describing coinfection of different coronavirus genotypes in bats and coronavirus genotypes of diverse nucleotide variation in the same host. Such unique phenomena, and the unusual instability of ORF7a, are likely due to recombination which may have been facilitated by the dense roosting behavior and long foraging range of Leschenault&apos;s rousette. Copyright &#169; 2010, American Society for Microbiology. All Rights Reserved.</description.abstract>
<language>eng</language>
<publisher>American Society for Microbiology. The Journal&apos;s web site is located at http://jvi.asm.org/</publisher>
<relation.ispartof>Journal of Virology</relation.ispartof>
<rights>Journal of Virology. Copyright &#169; American Society for Microbiology.</rights>
<subject.mesh>Antibodies, Viral - blood</subject.mesh>
<subject.mesh>Chiroptera - virology</subject.mesh>
<subject.mesh>Comorbidity</subject.mesh>
<subject.mesh>Coronavirus - classification - genetics</subject.mesh>
<subject.mesh>Polymorphism, Genetic</subject.mesh>
<title>Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup</title>
<type>Article</type>
<description.nature>link_to_OA_fulltext</description.nature>
<identifier.doi>10.1128/JVI.01121-10</identifier.doi>
<identifier.pmid>20702646</identifier.pmid>
<identifier.pmcid>PMC2953156</identifier.pmcid>
<identifier.scopus>eid_2-s2.0-77957962420</identifier.scopus>
<identifier.hkuros>188637</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-77957962420&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>84</identifier.volume>
<identifier.issue>21</identifier.issue>
<identifier.spage>11385</identifier.spage>
<identifier.epage>11394</identifier.epage>
<identifier.isi>WOS:000282643400042</identifier.isi>
<publisher.place>United States</publisher.place>
<bitstream.url>http://hub.hku.hk/bitstream/10722/135279/1/re01.htm</bitstream.url>
</item>
Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. The University of Hong Kong