File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus

TitleAn H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
Citation
Virology Journal, 2010, v. 7 How to Cite?
AbstractBackground. A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. Results. Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. Conclusions. Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. © 2010 Zhao et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135277
ISSN
2014 Impact Factor: 2.181
2014 SCImago Journal Rankings: 0.905
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Natural Science Foundation of China30901371
Mega-projects of Science Research for the 11th Five-Year Plan2009ZX10004-401
National Basic Research Program of China (973 Program)2005CB523001
National Science and Technology Infrastructure Program2006BA06A15
Funding Information:

This study was supported by Natural Science Foundation of China (30901371), Mega-projects of Science Research for the 11th Five-Year Plan (2009ZX10004-401), National Basic Research Program of China (973 Program, 2005CB523001), and National Science and Technology Infrastructure Program (2006BA06A15).

References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorSun, Sen_HK
dc.contributor.authorDu, Len_HK
dc.contributor.authorXiao, Wen_HK
dc.contributor.authorRu, Zen_HK
dc.contributor.authorKou, Zen_HK
dc.contributor.authorGuo, Yen_HK
dc.contributor.authorYu, Hen_HK
dc.contributor.authorJiang, Sen_HK
dc.contributor.authorLone, Yen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorZhou, Yen_HK
dc.date.accessioned2011-07-27T01:31:05Z-
dc.date.available2011-07-27T01:31:05Z-
dc.date.issued2010en_HK
dc.identifier.citationVirology Journal, 2010, v. 7en_HK
dc.identifier.issn1743-422Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135277-
dc.description.abstractBackground. A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. Results. Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. Conclusions. Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. © 2010 Zhao et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/en_HK
dc.relation.ispartofVirology Journalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.-
dc.subject.meshCross Protection-
dc.subject.meshDisease Outbreaks-
dc.subject.meshInfluenza Vaccines - administration and dosage - immunology-
dc.subject.meshInfluenza, Human - epidemiology - immunology - prevention and control - virology-
dc.subject.meshViral Matrix Proteins - administration and dosage - chemistry - immunology-
dc.titleAn H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virusen_HK
dc.typeArticleen_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1743-422X-7-151en_HK
dc.identifier.pmid20624292-
dc.identifier.pmcidPMC2912260-
dc.identifier.scopuseid_2-s2.0-77954409486en_HK
dc.identifier.hkuros188635en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954409486&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.isiWOS:000282601300001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridSun, S=35171536200en_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridXiao, W=9245533300en_HK
dc.identifier.scopusauthoridRu, Z=36197231500en_HK
dc.identifier.scopusauthoridKou, Z=23034818200en_HK
dc.identifier.scopusauthoridGuo, Y=8555122500en_HK
dc.identifier.scopusauthoridYu, H=36349449900en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK
dc.identifier.scopusauthoridLone, Y=36196779400en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.citeulike7493888-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats