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Article: An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus

TitleAn H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
Citation
Virology Journal, 2010, v. 7 How to Cite?
Abstract
Background. A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. Results. Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. Conclusions. Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. © 2010 Zhao et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135277
ISSN
2013 Impact Factor: 2.089
2013 SCImago Journal Rankings: 1.023
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Natural Science Foundation of China30901371
Mega-projects of Science Research for the 11th Five-Year Plan2009ZX10004-401
National Basic Research Program of China (973 Program)2005CB523001
National Science and Technology Infrastructure Program2006BA06A15
Funding Information:

This study was supported by Natural Science Foundation of China (30901371), Mega-projects of Science Research for the 11th Five-Year Plan (2009ZX10004-401), National Basic Research Program of China (973 Program, 2005CB523001), and National Science and Technology Infrastructure Program (2006BA06A15).

References

 

Author Affiliations
  1. Institute of Microbiology Chinese Academy of Sciences
  2. The University of Hong Kong
  3. Universite Paris-Sud XI
  4. New York Blood Center
DC FieldValueLanguage
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorSun, Sen_HK
dc.contributor.authorDu, Len_HK
dc.contributor.authorXiao, Wen_HK
dc.contributor.authorRu, Zen_HK
dc.contributor.authorKou, Zen_HK
dc.contributor.authorGuo, Yen_HK
dc.contributor.authorYu, Hen_HK
dc.contributor.authorJiang, Sen_HK
dc.contributor.authorLone, Yen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorZhou, Yen_HK
dc.date.accessioned2011-07-27T01:31:05Z-
dc.date.available2011-07-27T01:31:05Z-
dc.date.issued2010en_HK
dc.identifier.citationVirology Journal, 2010, v. 7en_HK
dc.identifier.issn1743-422Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135277-
dc.description.abstractBackground. A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. Results. Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. Conclusions. Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. © 2010 Zhao et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/en_HK
dc.relation.ispartofVirology Journalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.-
dc.subject.meshCross Protection-
dc.subject.meshDisease Outbreaks-
dc.subject.meshInfluenza Vaccines - administration and dosage - immunology-
dc.subject.meshInfluenza, Human - epidemiology - immunology - prevention and control - virology-
dc.subject.meshViral Matrix Proteins - administration and dosage - chemistry - immunology-
dc.titleAn H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virusen_HK
dc.typeArticleen_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1743-422X-7-151en_HK
dc.identifier.pmid20624292-
dc.identifier.pmcidPMC2912260-
dc.identifier.scopuseid_2-s2.0-77954409486en_HK
dc.identifier.hkuros188635en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954409486&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.isiWOS:000282601300001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridSun, S=35171536200en_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridXiao, W=9245533300en_HK
dc.identifier.scopusauthoridRu, Z=36197231500en_HK
dc.identifier.scopusauthoridKou, Z=23034818200en_HK
dc.identifier.scopusauthoridGuo, Y=8555122500en_HK
dc.identifier.scopusauthoridYu, H=36349449900en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK
dc.identifier.scopusauthoridLone, Y=36196779400en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.citeulike7493888-

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