Article: A highly conserved neutralizing epitope on group 2 influenza A viruses

We thank H. Tien and D. Marciano of the Robotics Core at the Joint Center for Structural Genomics for automated crystal screening; T. Doukov and the staff of the SSRL BL9-2 for beamline support; X. Dai and R. Stanfield for excellent assistance with data collection, processing, and analyses; R. Lerner, J. Paulson, and D. Burton for valuable comments and insightful discussion; E. Geelen, D. Spek, and V. Klaren for excellent assistance and advice; K. Hegmans, A. Lourbakos, J. Meijer, and A. Apetri and their teams for producing the mAbs and Fabs; C. Y. H. Leung for providing the A/WF/Hong Kong/MPA892/06 virus; E. de Boer-Luijtze and technicians in the groups of P. van Rossum and S. Riemersma for assistance with the animal experiments; E. Brown from Ottawa University, Canada for the mouse-adapted A/Hong Kong/1/68 strain; and A. Dingemans for critical review of the manuscript. This project has been funded in part by the National Institute of Allergy and Infectious Diseases, NIH, Department of Health and Human Services, USA, under contract HHSN272200900060C; the Area of Excellence Scheme of the University Grants Committee, Hong Kong (grant AoE/M-12/06); a predoctoral fellowship from the Achievement Rewards for College Scientists Foundation (D. C. E.); grant GM080209 from the NIH Molecular Evolution Training Program (D. C. E.); and the Skaggs Institute (I. A. W.). Portions of this research were carried out at the Stanford Synchrotron Radiation Lightsource, a national user facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences. The Stanford Synchrotron Radiation Lightsource (SSRL) Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research and by NIH, National Center for Research Resources, Biomedical Technology Program, and the National Institute of General Medical Sciences. This is publication 20951 from the Scripps Research Institute. Coordinates and structure factors have been deposited in the Protein Data Bank (PDB code 3SDY). Nucleotide sequences for the CR8020 variable regions have been deposited in GenBank (accession nos. JN093122, JN093123). A patent application relating to antibody CR8020 has been filed (International Publication Number WO2010/130636). Sharing of materials will be subject to standard material transfer agreements.