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Article: DNA intercalator stimulates influenza transcription and virus replication
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TitleDNA intercalator stimulates influenza transcription and virus replication
 
AuthorsLi, OTW1
Poon, LLM1
 
Issue Date2011
 
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
 
CitationVirology Journal, 2011, v. 8 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1743-422X-8-120
 
AbstractInfluenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd.
 
ISSN1743-422X
2012 Impact Factor: 2.092
2012 SCImago Journal Rankings: 0.798
 
DOIhttp://dx.doi.org/10.1186/1743-422X-8-120
 
PubMed Central IDPMC3069954
 
ISI Accession Number IDWOS:000289172500001
Funding AgencyGrant Number
National Institutes of Health (NIAID)HHSN266200700005C
Research Grant Council of Hong KongHKU 7530/06M
University Grants CommitteeAoE/M-12/06
Funding Information:

This project is supported by National Institutes of Health (NIAID contract HHSN266200700005C, LLM), Research Grant Council of Hong Kong (HKU 7530/06M, LLM), Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06). We thank George Brownlee for plasmids, Ervin Fodor and Hui-Ling Yen for critical comments.

 
ReferencesReferences in Scopus
 
GrantsControl of Pandemic and Inter-pandemic Influenza
Studies of mammalian, avian and chimeric influenza polymerase complexes
 
DC FieldValue
dc.contributor.authorLi, OTW
 
dc.contributor.authorPoon, LLM
 
dc.date.accessioned2011-07-27T01:30:56Z
 
dc.date.available2011-07-27T01:30:56Z
 
dc.date.issued2011
 
dc.description.abstractInfluenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationVirology Journal, 2011, v. 8 [How to Cite?]
DOI: http://dx.doi.org/10.1186/1743-422X-8-120
 
dc.identifier.citeulike8993642
 
dc.identifier.doihttp://dx.doi.org/10.1186/1743-422X-8-120
 
dc.identifier.hkuros188537
 
dc.identifier.isiWOS:000289172500001
Funding AgencyGrant Number
National Institutes of Health (NIAID)HHSN266200700005C
Research Grant Council of Hong KongHKU 7530/06M
University Grants CommitteeAoE/M-12/06
Funding Information:

This project is supported by National Institutes of Health (NIAID contract HHSN266200700005C, LLM), Research Grant Council of Hong Kong (HKU 7530/06M, LLM), Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06). We thank George Brownlee for plasmids, Ervin Fodor and Hui-Ling Yen for critical comments.

 
dc.identifier.issn1743-422X
2012 Impact Factor: 2.092
2012 SCImago Journal Rankings: 0.798
 
dc.identifier.pmcidPMC3069954
 
dc.identifier.pmid21401962
 
dc.identifier.scopuseid_2-s2.0-79952547974
 
dc.identifier.urihttp://hdl.handle.net/10722/135271
 
dc.identifier.volume8
 
dc.languageeng
 
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofVirology Journal
 
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza
 
dc.relation.projectStudies of mammalian, avian and chimeric influenza polymerase complexes
 
dc.relation.referencesReferences in Scopus
 
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshGene Expression Regulation, Viral - drug effects
 
dc.subject.meshInfluenza A virus - drug effects - enzymology - genetics - physiology
 
dc.subject.meshIntercalating Agents - pharmacology
 
dc.subject.meshTranscription, Genetic - drug effects
 
dc.subject.meshVirus Replication - drug effects
 
dc.titleDNA intercalator stimulates influenza transcription and virus replication
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong