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Article: DNA intercalator stimulates influenza transcription and virus replication

TitleDNA intercalator stimulates influenza transcription and virus replication
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/
Citation
Virology Journal, 2011, v. 8 How to Cite?
Abstract
Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135271
ISSN
2013 Impact Factor: 2.089
2013 SCImago Journal Rankings: 1.023
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of Health (NIAID)HHSN266200700005C
Research Grant Council of Hong KongHKU 7530/06M
University Grants CommitteeAoE/M-12/06
Funding Information:

This project is supported by National Institutes of Health (NIAID contract HHSN266200700005C, LLM), Research Grant Council of Hong Kong (HKU 7530/06M, LLM), Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06). We thank George Brownlee for plasmids, Ervin Fodor and Hui-Ling Yen for critical comments.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLi, OTWen_HK
dc.contributor.authorPoon, LLMen_HK
dc.date.accessioned2011-07-27T01:30:56Z-
dc.date.available2011-07-27T01:30:56Z-
dc.date.issued2011en_HK
dc.identifier.citationVirology Journal, 2011, v. 8en_HK
dc.identifier.issn1743-422Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135271-
dc.description.abstractInfluenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/en_HK
dc.relation.ispartofVirology Journalen_HK
dc.rightsVirology Journal. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshGene Expression Regulation, Viral - drug effects-
dc.subject.meshInfluenza A virus - drug effects - enzymology - genetics - physiology-
dc.subject.meshIntercalating Agents - pharmacology-
dc.subject.meshTranscription, Genetic - drug effects-
dc.subject.meshVirus Replication - drug effects-
dc.titleDNA intercalator stimulates influenza transcription and virus replicationen_HK
dc.typeArticleen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1743-422X-8-120en_HK
dc.identifier.pmid21401962-
dc.identifier.pmcidPMC3069954-
dc.identifier.scopuseid_2-s2.0-79952547974en_HK
dc.identifier.hkuros188537en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952547974&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.isiWOS:000289172500001-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.relation.projectStudies of mammalian, avian and chimeric influenza polymerase complexes-
dc.identifier.scopusauthoridLi, OTW=16230718400en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.citeulike8993642-

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