Article: DNA intercalator stimulates influenza transcription and virus replication
| Title | DNA intercalator stimulates influenza transcription and virus replication | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Li, OTW1 Poon, LLM1 | ||||||||
| Issue Date | 2011 | ||||||||
| Publisher | BioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/ | ||||||||
| Citation | Virology Journal, 2011, v. 8 [How to Cite?] DOI: http://dx.doi.org/10.1186/1743-422X-8-120 | ||||||||
| Abstract | Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd. | ||||||||
| ISSN | 1743-422X 2011 Impact Factor: 2.343 2011 SCImago Journal Rankings: 0.216 | ||||||||
| DOI | http://dx.doi.org/10.1186/1743-422X-8-120 | ||||||||
| ISI Accession Number ID | WOS:000289172500001
Funding Information: This project is supported by National Institutes of Health (NIAID contract HHSN266200700005C, LLM), Research Grant Council of Hong Kong (HKU 7530/06M, LLM), Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06). We thank George Brownlee for plasmids, Ervin Fodor and Hui-Ling Yen for critical comments. | ||||||||
| PubMed Central ID | PMC3069954 | ||||||||
| References | References in Scopus | ||||||||
| Grants | Control of Pandemic and Inter-pandemic Influenza Studies of mammalian, avian and chimeric influenza polymerase complexes |
| dc.contributor.author | Li, OTW | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Poon, LLM | ||||||||
| dc.date.accessioned | 2011-07-27T01:30:56Z | ||||||||
| dc.date.available | 2011-07-27T01:30:56Z | ||||||||
| dc.date.issued | 2011 | ||||||||
| dc.description.abstract | Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII a in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII a) to hyperphosphorylated RNAPII (RNAPII o). © 2011 Li and Poon; licensee BioMed Central Ltd. | ||||||||
| dc.description.grant | Control of Pandemic and Inter-pandemic Influenza | ||||||||
| dc.description.grant | Studies of mammalian, avian and chimeric influenza polymerase complexes | ||||||||
| dc.description.grantcode | 97655 | ||||||||
| dc.description.grantcode | 82370 | ||||||||
| dc.description.nature | published_or_final_version | ||||||||
| dc.identifier.citation | Virology Journal, 2011, v. 8 [How to Cite?] DOI: http://dx.doi.org/10.1186/1743-422X-8-120 | ||||||||
| dc.identifier.citeulike | 8993642 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1186/1743-422X-8-120 | ||||||||
| dc.identifier.hkuros | 188537 | ||||||||
| dc.identifier.isi | WOS:000289172500001
Funding Information: This project is supported by National Institutes of Health (NIAID contract HHSN266200700005C, LLM), Research Grant Council of Hong Kong (HKU 7530/06M, LLM), Area of Excellence Scheme of the University Grants Committee (Grant AoE/M-12/06). We thank George Brownlee for plasmids, Ervin Fodor and Hui-Ling Yen for critical comments. | ||||||||
| dc.identifier.issn | 1743-422X 2011 Impact Factor: 2.343 2011 SCImago Journal Rankings: 0.216 | ||||||||
| dc.identifier.pmcid | PMC3069954 | ||||||||
| dc.identifier.pmid | 21401962 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-79952547974 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/135271 | ||||||||
| dc.identifier.volume | 8 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | BioMed Central Ltd. The Journal's web site is located at http://www.virologyj.com/home/ | ||||||||
| dc.publisher.place | United Kingdom | ||||||||
| dc.relation.ispartof | Virology Journal | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.rights | Virology Journal. Copyright © BioMed Central Ltd. | ||||||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||||||
| dc.subject.mesh | Gene Expression Regulation, Viral - drug effects | ||||||||
| dc.subject.mesh | Influenza A virus - drug effects - enzymology - genetics - physiology | ||||||||
| dc.subject.mesh | Intercalating Agents - pharmacology | ||||||||
| dc.subject.mesh | Transcription, Genetic - drug effects | ||||||||
| dc.subject.mesh | Virus Replication - drug effects | ||||||||
| dc.title | DNA intercalator stimulates influenza transcription and virus replication | ||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong