Article: Detection of SARS coronavirus.

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- Professor Peiris, Joseph Sriyal Malik
- Dr Poon, Leo Lit Man

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Publisher Website: 10.1007/978-1-60761-817-1_20
Scopus: eid_2-s2.0-79955849665
PMID: 21116811

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Title	Detection of SARS coronavirus.
Authors	Peiris, JS1 Poon, LL
Issue Date	2011
Publisher	Humana Press, Inc.
Citation	Methods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382 [How to Cite?] DOI: http://dx.doi.org/10.1007/978-1-60761-817-1_20
Abstract	The emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
ISSN	1940-6029
DOI	http://dx.doi.org/10.1007/978-1-60761-817-1_20

DC Field	Value
dc.contributor.author	Peiris, JS
dc.contributor.author	Poon, LL
dc.date.accessioned	2011-07-27T01:30:55Z
dc.date.available	2011-07-27T01:30:55Z
dc.date.issued	2011
dc.description.abstract	The emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
dc.description.nature	Link_to_subscribed_fulltext
dc.identifier.citation	Methods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382 [How to Cite?] DOI: http://dx.doi.org/10.1007/978-1-60761-817-1_20
dc.identifier.doi	http://dx.doi.org/10.1007/978-1-60761-817-1_20
dc.identifier.epage	382
dc.identifier.hkuros	188535
dc.identifier.issn	1940-6029
dc.identifier.pmid	21116811
dc.identifier.scopus	eid_2-s2.0-79955849665
dc.identifier.spage	369
dc.identifier.uri	http://hdl.handle.net/10722/135270
dc.identifier.volume	665
dc.language	eng
dc.publisher	Humana Press, Inc.
dc.publisher.place	United States
dc.relation.ispartof	Methods in molecular biology (Clifton, N.J.)
dc.rights	The original publication is available at www.springerlink.com
dc.subject.mesh	Coronavirus - genetics
dc.subject.mesh	Polymerase Chain Reaction - methods
dc.subject.mesh	RNA, Viral - genetics - isolation and purification
dc.subject.mesh	SARS Virus - genetics
dc.subject.mesh	Severe Acute Respiratory Syndrome - diagnosis - epidemiology - virology
dc.title	Detection of SARS coronavirus.
dc.type	Article

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The University of Hong Kong

Article: Detection of SARS coronavirus.

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