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Article: Detection of SARS coronavirus.

TitleDetection of SARS coronavirus.
Authors
Issue Date2011
PublisherHumana Press, Inc.
Citation
Methods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382 How to Cite?
Abstract
The emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
Persistent Identifierhttp://hdl.handle.net/10722/135270
ISSN

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorPeiris, JSen_HK
dc.contributor.authorPoon, LLen_HK
dc.date.accessioned2011-07-27T01:30:55Z-
dc.date.available2011-07-27T01:30:55Z-
dc.date.issued2011en_HK
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382en_HK
dc.identifier.issn1940-6029en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135270-
dc.description.abstractThe emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.en_HK
dc.languageengen_US
dc.publisherHumana Press, Inc.-
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshCoronavirus - genetics-
dc.subject.meshPolymerase Chain Reaction - methods-
dc.subject.meshRNA, Viral - genetics - isolation and purification-
dc.subject.meshSARS Virus - genetics-
dc.subject.meshSevere Acute Respiratory Syndrome - diagnosis - epidemiology - virology-
dc.titleDetection of SARS coronavirus.en_HK
dc.typeArticleen_HK
dc.identifier.emailPeiris, JS: malik@hkucc.hku.hken_HK
dc.identifier.emailPoon, LL: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JS=rp00410en_HK
dc.identifier.authorityPoon, LL=rp00484en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-60761-817-1_20-
dc.identifier.pmid21116811en_HK
dc.identifier.scopuseid_2-s2.0-79955849665en_HK
dc.identifier.hkuros188535en_US
dc.identifier.volume665en_HK
dc.identifier.spage369en_HK
dc.identifier.epage382en_HK
dc.publisher.placeUnited States-
dc.identifier.scopusauthoridPeiris, JS=7005486823en_HK
dc.identifier.scopusauthoridPoon, LL=7005441747en_HK

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