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Article: Detection of SARS coronavirus.
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TitleDetection of SARS coronavirus.
 
AuthorsPeiris, JS1
Poon, LL
 
Issue Date2011
 
PublisherHumana Press, Inc.
 
CitationMethods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382 [How to Cite?]
DOI: http://dx.doi.org/10.1007/978-1-60761-817-1_20
 
AbstractThe emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
 
ISSN1940-6029
 
DOIhttp://dx.doi.org/10.1007/978-1-60761-817-1_20
 
DC FieldValue
dc.contributor.authorPeiris, JS
 
dc.contributor.authorPoon, LL
 
dc.date.accessioned2011-07-27T01:30:55Z
 
dc.date.available2011-07-27T01:30:55Z
 
dc.date.issued2011
 
dc.description.abstractThe emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2011, v. 665, p. 369-382 [How to Cite?]
DOI: http://dx.doi.org/10.1007/978-1-60761-817-1_20
 
dc.identifier.doihttp://dx.doi.org/10.1007/978-1-60761-817-1_20
 
dc.identifier.epage382
 
dc.identifier.hkuros188535
 
dc.identifier.issn1940-6029
 
dc.identifier.pmid21116811
 
dc.identifier.scopuseid_2-s2.0-79955849665
 
dc.identifier.spage369
 
dc.identifier.urihttp://hdl.handle.net/10722/135270
 
dc.identifier.volume665
 
dc.languageeng
 
dc.publisherHumana Press, Inc.
 
dc.publisher.placeUnited States
 
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)
 
dc.rightsThe original publication is available at www.springerlink.com
 
dc.subject.meshCoronavirus - genetics
 
dc.subject.meshPolymerase Chain Reaction - methods
 
dc.subject.meshRNA, Viral - genetics - isolation and purification
 
dc.subject.meshSARS Virus - genetics
 
dc.subject.meshSevere Acute Respiratory Syndrome - diagnosis - epidemiology - virology
 
dc.titleDetection of SARS coronavirus.
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong