Article: SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
| Title | SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway | ||||
|---|---|---|---|---|---|
| Authors | Keng, CT1 Åkerström, S3 5 Leung, CSW4 Poon, LLM4 Peiris, JSM4 6 Mirazimi, A3 5 Tan, YJ1 2 | ||||
| Keywords | 8b protein Accessory proteins Envelope (E) Proteasome pathway Severe acute respiratory syndrome coronavirus (SARS-CoV) Ubiquitin-independent | ||||
| Issue Date | 2011 | ||||
| Publisher | Elsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinf | ||||
| Citation | Microbes And Infection, 2011, v. 13 n. 2, p. 179-188 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.micinf.2010.10.017 | ||||
| Abstract | The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur. | ||||
| ISSN | 1286-4579 2011 Impact Factor: 3.101 2011 SCImago Journal Rankings: 0.293 | ||||
| DOI | http://dx.doi.org/10.1016/j.micinf.2010.10.017 | ||||
| ISI Accession Number ID | WOS:000287347000008
Funding Information: This work was supported by a grant from the Agency for Science, Technology and Research (A*STAR), Singapore. | ||||
| References | References in Scopus |
| dc.contributor.author | Keng, CT | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Åkerström, S | ||||
| dc.contributor.author | Leung, CSW | ||||
| dc.contributor.author | Poon, LLM | ||||
| dc.contributor.author | Peiris, JSM | ||||
| dc.contributor.author | Mirazimi, A | ||||
| dc.contributor.author | Tan, YJ | ||||
| dc.date.accessioned | 2011-07-27T01:30:55Z | ||||
| dc.date.available | 2011-07-27T01:30:55Z | ||||
| dc.date.issued | 2011 | ||||
| dc.description.abstract | The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Microbes And Infection, 2011, v. 13 n. 2, p. 179-188 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.micinf.2010.10.017 | ||||
| dc.identifier.citeulike | 8184898 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.micinf.2010.10.017 | ||||
| dc.identifier.epage | 188 | ||||
| dc.identifier.hkuros | 188533 | ||||
| dc.identifier.isi | WOS:000287347000008
Funding Information: This work was supported by a grant from the Agency for Science, Technology and Research (A*STAR), Singapore. | ||||
| dc.identifier.issn | 1286-4579 2011 Impact Factor: 3.101 2011 SCImago Journal Rankings: 0.293 | ||||
| dc.identifier.issue | 2 | ||||
| dc.identifier.pmid | 21035562 | ||||
| dc.identifier.scopus | eid_2-s2.0-78651508191 | ||||
| dc.identifier.spage | 179 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/135269 | ||||
| dc.identifier.volume | 13 | ||||
| dc.language | eng | ||||
| dc.publisher | Elsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinf | ||||
| dc.publisher.place | France | ||||
| dc.relation.ispartof | Microbes and Infection | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject.mesh | Proteasome Endopeptidase Complex - metabolism | ||||
| dc.subject.mesh | SARS Virus - genetics - metabolism - physiology | ||||
| dc.subject.mesh | Viral Envelope Proteins - metabolism | ||||
| dc.subject.mesh | Viral Proteins - metabolism | ||||
| dc.subject.mesh | Virus Replication - genetics | ||||
| dc.subject | 8b protein | ||||
| dc.subject | Accessory proteins | ||||
| dc.subject | Envelope (E) | ||||
| dc.subject | Proteasome pathway | ||||
| dc.subject | Severe acute respiratory syndrome coronavirus (SARS-CoV) | ||||
| dc.subject | Ubiquitin-independent | ||||
| dc.title | SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway | ||||
| dc.type | Article |
Author Affiliations
- Institute of Molecular and Cell Biology, A-Star, Singapore
- Yong Loo Lin School of Medicine
- Karolinska University Hospital
- The University of Hong Kong
- Swedish Institute for Infectious Disease Control
- HKU-Pasteur Research Centre