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Article: SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
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TitleSARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
 
AuthorsKeng, CT1
Åkerström, S5 2
Leung, CSW4
Poon, LLM4
Peiris, JSM6 4
Mirazimi, A5 2
Tan, YJ1 3
 
Keywords8b protein
Accessory proteins
Envelope (E)
Proteasome pathway
Severe acute respiratory syndrome coronavirus (SARS-CoV)
Ubiquitin-independent
 
Issue Date2011
 
PublisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinf
 
CitationMicrobes And Infection, 2011, v. 13 n. 2, p. 179-188 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.micinf.2010.10.017
 
AbstractThe severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur.
 
ISSN1286-4579
2013 Impact Factor: 2.731
2013 SCImago Journal Rankings: 1.507
 
DOIhttp://dx.doi.org/10.1016/j.micinf.2010.10.017
 
ISI Accession Number IDWOS:000287347000008
Funding AgencyGrant Number
Agency for Science, Technology and Research (A*STAR), Singapore
Funding Information:

This work was supported by a grant from the Agency for Science, Technology and Research (A*STAR), Singapore.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKeng, CT
 
dc.contributor.authorÅkerström, S
 
dc.contributor.authorLeung, CSW
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorMirazimi, A
 
dc.contributor.authorTan, YJ
 
dc.date.accessioned2011-07-27T01:30:55Z
 
dc.date.available2011-07-27T01:30:55Z
 
dc.date.issued2011
 
dc.description.abstractThe severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMicrobes And Infection, 2011, v. 13 n. 2, p. 179-188 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.micinf.2010.10.017
 
dc.identifier.citeulike8184898
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.micinf.2010.10.017
 
dc.identifier.epage188
 
dc.identifier.hkuros188533
 
dc.identifier.isiWOS:000287347000008
Funding AgencyGrant Number
Agency for Science, Technology and Research (A*STAR), Singapore
Funding Information:

This work was supported by a grant from the Agency for Science, Technology and Research (A*STAR), Singapore.

 
dc.identifier.issn1286-4579
2013 Impact Factor: 2.731
2013 SCImago Journal Rankings: 1.507
 
dc.identifier.issue2
 
dc.identifier.pmid21035562
 
dc.identifier.scopuseid_2-s2.0-78651508191
 
dc.identifier.spage179
 
dc.identifier.urihttp://hdl.handle.net/10722/135269
 
dc.identifier.volume13
 
dc.languageeng
 
dc.publisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinf
 
dc.publisher.placeFrance
 
dc.relation.ispartofMicrobes and Infection
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshProteasome Endopeptidase Complex - metabolism
 
dc.subject.meshSARS Virus - genetics - metabolism - physiology
 
dc.subject.meshViral Envelope Proteins - metabolism
 
dc.subject.meshViral Proteins - metabolism
 
dc.subject.meshVirus Replication - genetics
 
dc.subject8b protein
 
dc.subjectAccessory proteins
 
dc.subjectEnvelope (E)
 
dc.subjectProteasome pathway
 
dc.subjectSevere acute respiratory syndrome coronavirus (SARS-CoV)
 
dc.subjectUbiquitin-independent
 
dc.titleSARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
 
dc.typeArticle
 
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Author Affiliations
  1. Institute of Molecular and Cell Biology, A-Star, Singapore
  2. Karolinska University Hospital
  3. Yong Loo Lin School of Medicine
  4. The University of Hong Kong
  5. Swedish Institute for Infectious Disease Control
  6. HKU-Pasteur Research Centre