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Article: SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

TitleSARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
Authors
Keywords8b protein
Accessory proteins
Envelope (E)
Proteasome pathway
Severe acute respiratory syndrome coronavirus (SARS-CoV)
Ubiquitin-independent
Issue Date2011
PublisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinf
Citation
Microbes And Infection, 2011, v. 13 n. 2, p. 179-188 How to Cite?
AbstractThe severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur.
Persistent Identifierhttp://hdl.handle.net/10722/135269
ISSN
2014 Impact Factor: 2.861
2013 SCImago Journal Rankings: 1.507
ISI Accession Number ID
Funding AgencyGrant Number
Agency for Science, Technology and Research (A*STAR), Singapore
Funding Information:

This work was supported by a grant from the Agency for Science, Technology and Research (A*STAR), Singapore.

References

 

DC FieldValueLanguage
dc.contributor.authorKeng, CTen_HK
dc.contributor.authorÅkerström, Sen_HK
dc.contributor.authorLeung, CSWen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorMirazimi, Aen_HK
dc.contributor.authorTan, YJen_HK
dc.date.accessioned2011-07-27T01:30:55Z-
dc.date.available2011-07-27T01:30:55Z-
dc.date.issued2011en_HK
dc.identifier.citationMicrobes And Infection, 2011, v. 13 n. 2, p. 179-188en_HK
dc.identifier.issn1286-4579en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135269-
dc.description.abstractThe severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway. © 2010 Institut Pasteur.en_HK
dc.languageengen_US
dc.publisherElsevier France, Editions Scientifiques et Medicales. The Journal's web site is located at http://www.elsevier.com/locate/micinfen_HK
dc.relation.ispartofMicrobes and Infectionen_HK
dc.subject8b proteinen_HK
dc.subjectAccessory proteinsen_HK
dc.subjectEnvelope (E)en_HK
dc.subjectProteasome pathwayen_HK
dc.subjectSevere acute respiratory syndrome coronavirus (SARS-CoV)en_HK
dc.subjectUbiquitin-independenten_HK
dc.subject.meshProteasome Endopeptidase Complex - metabolism-
dc.subject.meshSARS Virus - genetics - metabolism - physiology-
dc.subject.meshViral Envelope Proteins - metabolism-
dc.subject.meshViral Proteins - metabolism-
dc.subject.meshVirus Replication - genetics-
dc.titleSARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathwayen_HK
dc.typeArticleen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.micinf.2010.10.017en_HK
dc.identifier.pmid21035562en_HK
dc.identifier.scopuseid_2-s2.0-78651508191en_HK
dc.identifier.hkuros188533en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78651508191&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue2en_HK
dc.identifier.spage179en_HK
dc.identifier.epage188en_HK
dc.identifier.isiWOS:000287347000008-
dc.publisher.placeFranceen_HK
dc.identifier.scopusauthoridKeng, CT=8280326200en_HK
dc.identifier.scopusauthoridÅkerström, S=8318387600en_HK
dc.identifier.scopusauthoridLeung, CSW=7402612654en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridMirazimi, A=6602224342en_HK
dc.identifier.scopusauthoridTan, YJ=7402139791en_HK
dc.identifier.citeulike8184898-

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