Article: Analysis of multilocus sequence typing schemes for 35 different bacteria revealed that gene loci of 10 bacteria could be replaced to improve cost-effectiveness
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- Publisher Website: 10.1016/j.diagmicrobio.2011.03.006
- Scopus: eid_2-s2.0-79959241403
- PMID: 21558049
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| Title | Analysis of multilocus sequence typing schemes for 35 different bacteria revealed that gene loci of 10 bacteria could be replaced to improve cost-effectiveness | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Woo, PCY1 Tsang, AKL1 Wong, AYP1 Chen, H2 Chu, J1 Lau, SKP1 Yuen, KY1 | ||||||
| Keywords | Bacteria Gene MLST Typing | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio | ||||||
| Citation | Diagnostic Microbiology And Infectious Disease, 2011, v. 70 n. 3, p. 316-323 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.diagmicrobio.2011.03.006 | ||||||
| Abstract | Although multilocus sequence typing (MLST) has been widely used for bacterial typing, the contribution of the gene loci to the discriminatory power of each MLST scheme is unknown. We analyzed the discriminatory powers of 36 MLST schemes using all combinations of the 7 loci and contributions of each locus to the schemes. In 10 schemes, sequencing 6 loci can achieve the discriminatory powers of 7 loci. For the other 26 schemes, the median marginal increase in discriminatory power when 7 instead of 6 loci were used is 0.0004. Sequencing the 7 loci of 50 strains each of Pseudomonas aeruginosa and Acinetobacter baumannii revealed that the discriminatory power for P. aeruginosa was 0.9861 when either 6 (without trp) or 7 loci were used and that for A. baumannii was 0.9363 when 5, 6, or 7 loci were used. Genes that have no additional or minimal contribution to the overall discriminatory powers should be replaced. © 2011 Elsevier Inc. | ||||||
| ISSN | 0732-8893 2011 Impact Factor: 2.528 2011 SCImago Journal Rankings: 0.210 | ||||||
| DOI | http://dx.doi.org/10.1016/j.diagmicrobio.2011.03.006 | ||||||
| ISI Accession Number ID | WOS:000292441100005
Funding Information: This work is partly supported by the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau, Research Grants Council Grant and University Development Fund, The University of Hong Kong. | ||||||
| References | References in Scopus |
| dc.contributor.author | Woo, PCY | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Tsang, AKL | ||||||
| dc.contributor.author | Wong, AYP | ||||||
| dc.contributor.author | Chen, H | ||||||
| dc.contributor.author | Chu, J | ||||||
| dc.contributor.author | Lau, SKP | ||||||
| dc.contributor.author | Yuen, KY | ||||||
| dc.date.accessioned | 2011-07-27T01:30:52Z | ||||||
| dc.date.available | 2011-07-27T01:30:52Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Although multilocus sequence typing (MLST) has been widely used for bacterial typing, the contribution of the gene loci to the discriminatory power of each MLST scheme is unknown. We analyzed the discriminatory powers of 36 MLST schemes using all combinations of the 7 loci and contributions of each locus to the schemes. In 10 schemes, sequencing 6 loci can achieve the discriminatory powers of 7 loci. For the other 26 schemes, the median marginal increase in discriminatory power when 7 instead of 6 loci were used is 0.0004. Sequencing the 7 loci of 50 strains each of Pseudomonas aeruginosa and Acinetobacter baumannii revealed that the discriminatory power for P. aeruginosa was 0.9861 when either 6 (without trp) or 7 loci were used and that for A. baumannii was 0.9363 when 5, 6, or 7 loci were used. Genes that have no additional or minimal contribution to the overall discriminatory powers should be replaced. © 2011 Elsevier Inc. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Diagnostic Microbiology And Infectious Disease, 2011, v. 70 n. 3, p. 316-323 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.diagmicrobio.2011.03.006 | ||||||
| dc.identifier.citeulike | 9316589 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.diagmicrobio.2011.03.006 | ||||||
| dc.identifier.epage | 323 | ||||||
| dc.identifier.hkuros | 187230 | ||||||
| dc.identifier.isi | WOS:000292441100005
Funding Information: This work is partly supported by the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau, Research Grants Council Grant and University Development Fund, The University of Hong Kong. | ||||||
| dc.identifier.issn | 0732-8893 2011 Impact Factor: 2.528 2011 SCImago Journal Rankings: 0.210 | ||||||
| dc.identifier.issue | 3 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 21558049 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79959241403 | ||||||
| dc.identifier.spage | 316 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/135262 | ||||||
| dc.identifier.volume | 70 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/diagmicrobio | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Diagnostic Microbiology and Infectious Disease | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Bacteria | ||||||
| dc.subject | Gene | ||||||
| dc.subject | MLST | ||||||
| dc.subject | Typing | ||||||
| dc.title | Analysis of multilocus sequence typing schemes for 35 different bacteria revealed that gene loci of 10 bacteria could be replaced to improve cost-effectiveness | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Capital Medical University China