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Article: Characterization of sry-related hmg box group f genes in zebrafish hematopoiesis
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TitleCharacterization of sry-related hmg box group f genes in zebrafish hematopoiesis
 
AuthorsChung, MIS1
Ma, ACH1
Fung, TK1
Leung, AYH1
 
KeywordsBeta catenin
Green fluorescent protein
Angiogenesis
Cell migration
Embryo development
 
Issue Date2011
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
 
CitationExperimental Hematology, 2011, v. 39 n. 10, p. 986-998.e5 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.exphem.2011.06.010
 
AbstractObjective: The roles of Sry-related HMG box (Sox) genes in zebrafish hematopoiesis are not clearly defined. In this study, we have characterized the sequence homology, gene expression, hematopoietic functions, and regulation of sox genes in F group (SoxF) in zebrafish embryos. Materials and Methods: Expression of zebrafish SoxF genes were analyzed by whole-mount in situ hybridization, reverse transcription polymerase chain reaction, and real-time reverse transcription polymerase chain reaction of erythroid cells obtained from Tg(gata1:GFP) embryos by fluorescence-activated cell sorting. Roles of SoxF genes were analyzed in zebrafish embryos using morpholino knockdown and analyzed by whole-mount in situ hybridization and real-time reverse transcription polymerase chain reaction. Embryo patterning and vascular development were analyzed. Results: All members, except sox17, contained a putative β-catenin binding site. sox7 and 18 expressed primarily in the vasculature. sox17 expressed in the intermediate cell mass and its knockdown significantly reduced primitive erythropoiesis at 18 hours post-fertilization (hpf). Definitive hematopoiesis was unaffected. Concomitant sox7 and sox18 knockdown disrupted vasculogenesis and angiogenesis, but not hematopoiesis. sox32 knockdown delayed medial migration of hematopoietic and endothelial progenitors at 18 hpf and abolished cmyb expression at the caudal hematopoietic tissue at 48 hpf. These defects could be prevented by delaying its knockdown using a caged sox32 morpholino uncaged at 10 hpf. Knockdown of SoxF genes significantly upregulated their own expression and that of sox32 also upregulated sox18 expression. Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. © 2011 ISEH - Society for Hematology and Stem Cells.
 
ISSN0301-472X
2012 Impact Factor: 2.907
2012 SCImago Journal Rankings: 1.248
 
DOIhttp://dx.doi.org/10.1016/j.exphem.2011.06.010
 
ISI Accession Number IDWOS:000295427300004
Funding AgencyGrant Number
General Research FundHKU 7520/06 M
HKU 770308 M
HKU 771611 M
University of Hong Kong
Funding Information:

The project was supported by the General Research Fund (HKU 7520/06 M, HKU 770308 M and HKU 771611 M), a grant from the strategy research theme of cancer stem cells in the University of Hong Kong and a small project funding from The University of Hong Kong. Confocal microscopy was kindly provided by the Faculty Core Facility (LKS Faculty of Medicine, University of Hong Kong, Hong Kong, China).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChung, MIS
 
dc.contributor.authorMa, ACH
 
dc.contributor.authorFung, TK
 
dc.contributor.authorLeung, AYH
 
dc.date.accessioned2011-07-27T01:30:27Z
 
dc.date.available2011-07-27T01:30:27Z
 
dc.date.issued2011
 
dc.description.abstractObjective: The roles of Sry-related HMG box (Sox) genes in zebrafish hematopoiesis are not clearly defined. In this study, we have characterized the sequence homology, gene expression, hematopoietic functions, and regulation of sox genes in F group (SoxF) in zebrafish embryos. Materials and Methods: Expression of zebrafish SoxF genes were analyzed by whole-mount in situ hybridization, reverse transcription polymerase chain reaction, and real-time reverse transcription polymerase chain reaction of erythroid cells obtained from Tg(gata1:GFP) embryos by fluorescence-activated cell sorting. Roles of SoxF genes were analyzed in zebrafish embryos using morpholino knockdown and analyzed by whole-mount in situ hybridization and real-time reverse transcription polymerase chain reaction. Embryo patterning and vascular development were analyzed. Results: All members, except sox17, contained a putative β-catenin binding site. sox7 and 18 expressed primarily in the vasculature. sox17 expressed in the intermediate cell mass and its knockdown significantly reduced primitive erythropoiesis at 18 hours post-fertilization (hpf). Definitive hematopoiesis was unaffected. Concomitant sox7 and sox18 knockdown disrupted vasculogenesis and angiogenesis, but not hematopoiesis. sox32 knockdown delayed medial migration of hematopoietic and endothelial progenitors at 18 hpf and abolished cmyb expression at the caudal hematopoietic tissue at 48 hpf. These defects could be prevented by delaying its knockdown using a caged sox32 morpholino uncaged at 10 hpf. Knockdown of SoxF genes significantly upregulated their own expression and that of sox32 also upregulated sox18 expression. Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. © 2011 ISEH - Society for Hematology and Stem Cells.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationExperimental Hematology, 2011, v. 39 n. 10, p. 986-998.e5 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.exphem.2011.06.010
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.exphem.2011.06.010
 
dc.identifier.epage998.e5
 
dc.identifier.hkuros188095
 
dc.identifier.isiWOS:000295427300004
Funding AgencyGrant Number
General Research FundHKU 7520/06 M
HKU 770308 M
HKU 771611 M
University of Hong Kong
Funding Information:

The project was supported by the General Research Fund (HKU 7520/06 M, HKU 770308 M and HKU 771611 M), a grant from the strategy research theme of cancer stem cells in the University of Hong Kong and a small project funding from The University of Hong Kong. Confocal microscopy was kindly provided by the Faculty Core Facility (LKS Faculty of Medicine, University of Hong Kong, Hong Kong, China).

 
dc.identifier.issn0301-472X
2012 Impact Factor: 2.907
2012 SCImago Journal Rankings: 1.248
 
dc.identifier.issue10
 
dc.identifier.openurl
 
dc.identifier.pmid21726513
 
dc.identifier.scopuseid_2-s2.0-80052860744
 
dc.identifier.spage986
 
dc.identifier.urihttp://hdl.handle.net/10722/135239
 
dc.identifier.volume39
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
 
dc.publisher.placeUnited States
 
dc.relation.ispartofExperimental Hematology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBeta catenin
 
dc.subjectGreen fluorescent protein
 
dc.subjectAngiogenesis
 
dc.subjectCell migration
 
dc.subjectEmbryo development
 
dc.titleCharacterization of sry-related hmg box group f genes in zebrafish hematopoiesis
 
dc.typeArticle
 
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<description.abstract>Objective: The roles of Sry-related HMG box (Sox) genes in zebrafish hematopoiesis are not clearly defined. In this study, we have characterized the sequence homology, gene expression, hematopoietic functions, and regulation of sox genes in F group (SoxF) in zebrafish embryos. Materials and Methods: Expression of zebrafish SoxF genes were analyzed by whole-mount in situ hybridization, reverse transcription polymerase chain reaction, and real-time reverse transcription polymerase chain reaction of erythroid cells obtained from Tg(gata1:GFP) embryos by fluorescence-activated cell sorting. Roles of SoxF genes were analyzed in zebrafish embryos using morpholino knockdown and analyzed by whole-mount in situ hybridization and real-time reverse transcription polymerase chain reaction. Embryo patterning and vascular development were analyzed. Results: All members, except sox17, contained a putative &#946;-catenin binding site. sox7 and 18 expressed primarily in the vasculature. sox17 expressed in the intermediate cell mass and its knockdown significantly reduced primitive erythropoiesis at 18 hours post-fertilization (hpf). Definitive hematopoiesis was unaffected. Concomitant sox7 and sox18 knockdown disrupted vasculogenesis and angiogenesis, but not hematopoiesis. sox32 knockdown delayed medial migration of hematopoietic and endothelial progenitors at 18 hpf and abolished cmyb expression at the caudal hematopoietic tissue at 48 hpf. These defects could be prevented by delaying its knockdown using a caged sox32 morpholino uncaged at 10 hpf. Knockdown of SoxF genes significantly upregulated their own expression and that of sox32 also upregulated sox18 expression. Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. &#169; 2011 ISEH - Society for Hematology and Stem Cells.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong