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Article: Characterization of sry-related hmg box group f genes in zebrafish hematopoiesis

TitleCharacterization of sry-related hmg box group f genes in zebrafish hematopoiesis
Authors
KeywordsBeta catenin
Green fluorescent protein
Angiogenesis
Cell migration
Embryo development
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2011, v. 39 n. 10, p. 986-998.e5 How to Cite?
Abstract
Objective: The roles of Sry-related HMG box (Sox) genes in zebrafish hematopoiesis are not clearly defined. In this study, we have characterized the sequence homology, gene expression, hematopoietic functions, and regulation of sox genes in F group (SoxF) in zebrafish embryos. Materials and Methods: Expression of zebrafish SoxF genes were analyzed by whole-mount in situ hybridization, reverse transcription polymerase chain reaction, and real-time reverse transcription polymerase chain reaction of erythroid cells obtained from Tg(gata1:GFP) embryos by fluorescence-activated cell sorting. Roles of SoxF genes were analyzed in zebrafish embryos using morpholino knockdown and analyzed by whole-mount in situ hybridization and real-time reverse transcription polymerase chain reaction. Embryo patterning and vascular development were analyzed. Results: All members, except sox17, contained a putative β-catenin binding site. sox7 and 18 expressed primarily in the vasculature. sox17 expressed in the intermediate cell mass and its knockdown significantly reduced primitive erythropoiesis at 18 hours post-fertilization (hpf). Definitive hematopoiesis was unaffected. Concomitant sox7 and sox18 knockdown disrupted vasculogenesis and angiogenesis, but not hematopoiesis. sox32 knockdown delayed medial migration of hematopoietic and endothelial progenitors at 18 hpf and abolished cmyb expression at the caudal hematopoietic tissue at 48 hpf. These defects could be prevented by delaying its knockdown using a caged sox32 morpholino uncaged at 10 hpf. Knockdown of SoxF genes significantly upregulated their own expression and that of sox32 also upregulated sox18 expression. Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. © 2011 ISEH - Society for Hematology and Stem Cells.
Persistent Identifierhttp://hdl.handle.net/10722/135239
ISSN
2013 Impact Factor: 2.806
2013 SCImago Journal Rankings: 1.462
ISI Accession Number ID
Funding AgencyGrant Number
General Research FundHKU 7520/06 M
HKU 770308 M
HKU 771611 M
University of Hong Kong
Funding Information:

The project was supported by the General Research Fund (HKU 7520/06 M, HKU 770308 M and HKU 771611 M), a grant from the strategy research theme of cancer stem cells in the University of Hong Kong and a small project funding from The University of Hong Kong. Confocal microscopy was kindly provided by the Faculty Core Facility (LKS Faculty of Medicine, University of Hong Kong, Hong Kong, China).

References

 

DC FieldValueLanguage
dc.contributor.authorChung, MISen_HK
dc.contributor.authorMa, ACHen_HK
dc.contributor.authorFung, TKen_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2011-07-27T01:30:27Z-
dc.date.available2011-07-27T01:30:27Z-
dc.date.issued2011en_HK
dc.identifier.citationExperimental Hematology, 2011, v. 39 n. 10, p. 986-998.e5en_HK
dc.identifier.issn0301-472Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135239-
dc.description.abstractObjective: The roles of Sry-related HMG box (Sox) genes in zebrafish hematopoiesis are not clearly defined. In this study, we have characterized the sequence homology, gene expression, hematopoietic functions, and regulation of sox genes in F group (SoxF) in zebrafish embryos. Materials and Methods: Expression of zebrafish SoxF genes were analyzed by whole-mount in situ hybridization, reverse transcription polymerase chain reaction, and real-time reverse transcription polymerase chain reaction of erythroid cells obtained from Tg(gata1:GFP) embryos by fluorescence-activated cell sorting. Roles of SoxF genes were analyzed in zebrafish embryos using morpholino knockdown and analyzed by whole-mount in situ hybridization and real-time reverse transcription polymerase chain reaction. Embryo patterning and vascular development were analyzed. Results: All members, except sox17, contained a putative β-catenin binding site. sox7 and 18 expressed primarily in the vasculature. sox17 expressed in the intermediate cell mass and its knockdown significantly reduced primitive erythropoiesis at 18 hours post-fertilization (hpf). Definitive hematopoiesis was unaffected. Concomitant sox7 and sox18 knockdown disrupted vasculogenesis and angiogenesis, but not hematopoiesis. sox32 knockdown delayed medial migration of hematopoietic and endothelial progenitors at 18 hpf and abolished cmyb expression at the caudal hematopoietic tissue at 48 hpf. These defects could be prevented by delaying its knockdown using a caged sox32 morpholino uncaged at 10 hpf. Knockdown of SoxF genes significantly upregulated their own expression and that of sox32 also upregulated sox18 expression. Conclusions: sox17 helped to maintain primitive hematopoiesis, whereas sox7 and sox18 regulated angiogenesis and vasculogenesis. sox32 affected both vascular and hematopoietic development through its effects on medial migration of the hematopoietic and endothelial progenitors. © 2011 ISEH - Society for Hematology and Stem Cells.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_HK
dc.relation.ispartofExperimental Hematologyen_HK
dc.subjectBeta catenin-
dc.subjectGreen fluorescent protein-
dc.subjectAngiogenesis-
dc.subjectCell migration-
dc.subjectEmbryo development-
dc.titleCharacterization of sry-related hmg box group f genes in zebrafish hematopoiesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-472X&volume=39&issue=10&spage=986&epage=998.e5&date=2011&atitle=Characterization+of+Sry-related+HMG+box+group+F+genes+in+zebrafish+hematopoiesis-
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exphem.2011.06.010en_HK
dc.identifier.pmid21726513en_HK
dc.identifier.scopuseid_2-s2.0-80052860744en_HK
dc.identifier.hkuros188095en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052860744&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue10en_HK
dc.identifier.spage986en_HK
dc.identifier.epage998.e5en_HK
dc.identifier.isiWOS:000295427300004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChung, MIS=25958659100en_HK
dc.identifier.scopusauthoridMa, ACH=52664122100en_HK
dc.identifier.scopusauthoridFung, TK=7102715924en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK

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