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Article: Cellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agents

TitleCellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agents
Authors
KeywordsCellular imaging
Gadonanotubes
Magnetic resonance imaging
MRI contrast agent
Single-walled carbon nanotubes
Issue Date2011
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/110577207
Citation
Contrast Media And Molecular Imaging, 2011, v. 6 n. 2, p. 93-99 How to Cite?
AbstractWe quantify here, for the first time, the intracellular uptake (J774A.1 murine macrophage cells) of gadolinium-loaded ultra-short single-walled carbon nanotubes (gadonanotubes or GNTs) in a 3 T MRI scanner using R 2 and R 2* mapping in vitro. GNT-labeled cells exhibited high and linear changes in net transverse relaxations (ΔR 2 and ΔR2*) with increasing cell concentration. The measured ΔR 2* were about three to four times greater than the respective ΔR 2 for each cell concentration. The intracellular uptake of GNTs was validated with inductively coupled plasma optical emission spectrometry (ICP-OES), indicating an average cellular uptake of 0.44±0.09pg Gd per cell or 1.69×10 9 Gd 3+ ions per cell. Cell proliferation MTS assays demonstrated that the cells were effectively labeled, without cytotoxicity, for GNTs concentrations ≤28μM Gd. In vivo relaxometry of a subcutaneously-injected GNT-labeled cell pellet in a mouse was also demonstrated at 3 T. Finally, the pronounced R 2* effect of GNT-labeled cells enabled successful in vitro visualization of labeled cells at 9.4 T. © 2010 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135099
ISSN
2015 Impact Factor: 3.286
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
Funding AgencyGrant Number
URC of the University of Hong Kong10208648
Robert A. Welch FoundationC-0627
Nanoscale Science and Engineering Initiative of the National Science Foundation at Rice UniversityEEC-067452
Methodist Hospital Research Institute
Funding Information:

This work was supported in part by the URC of the University of Hong Kong (grant no. 10208648), the Robert A. Welch Foundation (grant no. C-0627) and the Nanoscale Science and Engineering Initiative of the National Science Foundation (grant no. EEC-067452) at Rice University and the Methodist Hospital Research Institute. K. Wong would like to acknowledge the imaging support from Texas Children's Hospital small animal imaging facility.

References

 

DC FieldValueLanguage
dc.contributor.authorTang, AMen_HK
dc.contributor.authorAnanta, JSen_HK
dc.contributor.authorZhao, Hen_HK
dc.contributor.authorCisneros, BTen_HK
dc.contributor.authorLam, EYen_HK
dc.contributor.authorWong, STen_HK
dc.contributor.authorWilson, LJen_HK
dc.contributor.authorWong, KKen_HK
dc.date.accessioned2011-07-27T01:28:19Z-
dc.date.available2011-07-27T01:28:19Z-
dc.date.issued2011en_HK
dc.identifier.citationContrast Media And Molecular Imaging, 2011, v. 6 n. 2, p. 93-99en_HK
dc.identifier.issn1555-4309en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135099-
dc.description.abstractWe quantify here, for the first time, the intracellular uptake (J774A.1 murine macrophage cells) of gadolinium-loaded ultra-short single-walled carbon nanotubes (gadonanotubes or GNTs) in a 3 T MRI scanner using R 2 and R 2* mapping in vitro. GNT-labeled cells exhibited high and linear changes in net transverse relaxations (ΔR 2 and ΔR2*) with increasing cell concentration. The measured ΔR 2* were about three to four times greater than the respective ΔR 2 for each cell concentration. The intracellular uptake of GNTs was validated with inductively coupled plasma optical emission spectrometry (ICP-OES), indicating an average cellular uptake of 0.44±0.09pg Gd per cell or 1.69×10 9 Gd 3+ ions per cell. Cell proliferation MTS assays demonstrated that the cells were effectively labeled, without cytotoxicity, for GNTs concentrations ≤28μM Gd. In vivo relaxometry of a subcutaneously-injected GNT-labeled cell pellet in a mouse was also demonstrated at 3 T. Finally, the pronounced R 2* effect of GNT-labeled cells enabled successful in vitro visualization of labeled cells at 9.4 T. © 2010 John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/110577207en_HK
dc.relation.ispartofContrast Media and Molecular Imagingen_HK
dc.rightsContrast Media & Molecular Imaging. Copyright © John Wiley & Sons Ltd.-
dc.subjectCellular imagingen_HK
dc.subjectGadonanotubesen_HK
dc.subjectMagnetic resonance imagingen_HK
dc.subjectMRI contrast agenten_HK
dc.subjectSingle-walled carbon nanotubesen_HK
dc.subject.meshContrast Media - chemistry - metabolism-
dc.subject.meshGadolinium - chemistry-
dc.subject.meshMagnetic Resonance Imaging - methods-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshNanotubes, Carbon - chemistry-
dc.titleCellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agentsen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, EY:elam@eee.hku.hken_HK
dc.identifier.authorityLam, EY=rp00131en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cmmi.410en_HK
dc.identifier.pmid21504063-
dc.identifier.scopuseid_2-s2.0-79955044655en_HK
dc.identifier.hkuros186766en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955044655&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue2en_HK
dc.identifier.spage93en_HK
dc.identifier.epage99en_HK
dc.identifier.isiWOS:000289942600005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTang, AM=23390725500en_HK
dc.identifier.scopusauthoridAnanta, JS=24463663800en_HK
dc.identifier.scopusauthoridZhao, H=37098315700en_HK
dc.identifier.scopusauthoridCisneros, BT=47761012600en_HK
dc.identifier.scopusauthoridLam, EY=7102890004en_HK
dc.identifier.scopusauthoridWong, ST=12781047500en_HK
dc.identifier.scopusauthoridWilson, LJ=7403521631en_HK
dc.identifier.scopusauthoridWong, KK=35222708000en_HK

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