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Article: OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: Implication for a highly conserved plant cGTPase essential for chloroplast function

TitleOsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: Implication for a highly conserved plant cGTPase essential for chloroplast function
Authors
KeywordsChloroplast translation
NOA1/RIF1
Os02g01440
Plant cGTPase
Rice (Oryza sativa)
Issue Date2010
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH
Citation
New Phytologist, 2010, v. 187 n. 1, p. 83-105 How to Cite?
Abstract
Summary: •The bacterial protein YqeH is a circularly permuted GTPase with homologs encoded by plant nuclear genomes. The rice homolog OsNOA1/RIF1 is encoded by the single-copy gene Os02g01440. OsNOA1/RIF1 is expressed in different tissues and is light-inducible. The OsNOA1/RIF1-EYFP fusion protein was targeted to chloroplasts in transgenic Arabidopsis plants. In addition, the rice homolog was able to rescue most of the growth phenotypes in an Arabidopsis rif1 mutant.•Rice (Oryza sativa) OsNOA1/RIF1 RNAi mutant seedlings were chlorotic with reduced pigment contents and lower photosystem II (PSII) efficiency. However, the expressions of the chloroplast-encoded genes rbcL, atpB, psaA and psbA were not affected. By contrast, reduced abundance of the chloroplast 16S rRNA was observed in the mutant.•Quantitative iTRAQ-LC-MS/MS proteomics investigations revealed proteome changes in the rice mutant consistent with the expected functional role of OsNOA1/RIF1 in chloroplast translation. The RNAi mutant showed significantly decreased expression levels of chloroplast-encoded proteins as well as nuclear-encoded components of chloroplast enzyme complexes. Conversely, upregulation of some classes of nonchloroplastic proteins, such as glycolytic and phenylpropanoid pathway enzymes, was detected.•Our work provides independent indications that a highly conserved nuclear-encoded cGTPase of likely prokaryotic origin is essential for proper chloroplast ribosome assembly and/or translation in plants. © The Authors (2010). Journal compilation © New Phytologist Trust (2010).
Persistent Identifierhttp://hdl.handle.net/10722/135031
ISSN
ISI Accession Number ID
Funding AgencyGrant Number
National High Technology Research and Development Program of China2006AA10A102
Research Grants Council of Hong KongHKU7527/06M
HKU200811159080
Funding Information:

This work was supported by the National High Technology Research and Development Program of China (2006AA10A102) to Yuezhi Tao as well as the Research Grants Council of Hong Kong (HKU7527/06M) and HKU Seed Funding Programme (200811159080) to Clive Lo. We are grateful to Professor Jirong Huang (National Key Laboratory of Plant Molecular Genetics, Shanghai Institute for Biological Sciences) for his helpful discussions on our investigations. We also thank Dr P. Leon (Instituto de Biotechnologia-UNAM) for providing the DXS antibodies.

References

 

Author Affiliations
  1. Zhejiang Academy of Agricultural Sciences
  2. The University of Hong Kong
  3. School of Biological Sciences
  4. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorLau, Een_HK
dc.contributor.authorLam, MPYen_HK
dc.contributor.authorChu, Hen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorHuang, Gen_HK
dc.contributor.authorGuo, Pen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorJiang, Len_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorLo, Cen_HK
dc.contributor.authorTao, Yen_HK
dc.date.accessioned2011-07-27T01:26:19Z-
dc.date.available2011-07-27T01:26:19Z-
dc.date.issued2010en_HK
dc.identifier.citationNew Phytologist, 2010, v. 187 n. 1, p. 83-105en_HK
dc.identifier.issn0028-646Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135031-
dc.description.abstractSummary: •The bacterial protein YqeH is a circularly permuted GTPase with homologs encoded by plant nuclear genomes. The rice homolog OsNOA1/RIF1 is encoded by the single-copy gene Os02g01440. OsNOA1/RIF1 is expressed in different tissues and is light-inducible. The OsNOA1/RIF1-EYFP fusion protein was targeted to chloroplasts in transgenic Arabidopsis plants. In addition, the rice homolog was able to rescue most of the growth phenotypes in an Arabidopsis rif1 mutant.•Rice (Oryza sativa) OsNOA1/RIF1 RNAi mutant seedlings were chlorotic with reduced pigment contents and lower photosystem II (PSII) efficiency. However, the expressions of the chloroplast-encoded genes rbcL, atpB, psaA and psbA were not affected. By contrast, reduced abundance of the chloroplast 16S rRNA was observed in the mutant.•Quantitative iTRAQ-LC-MS/MS proteomics investigations revealed proteome changes in the rice mutant consistent with the expected functional role of OsNOA1/RIF1 in chloroplast translation. The RNAi mutant showed significantly decreased expression levels of chloroplast-encoded proteins as well as nuclear-encoded components of chloroplast enzyme complexes. Conversely, upregulation of some classes of nonchloroplastic proteins, such as glycolytic and phenylpropanoid pathway enzymes, was detected.•Our work provides independent indications that a highly conserved nuclear-encoded cGTPase of likely prokaryotic origin is essential for proper chloroplast ribosome assembly and/or translation in plants. © The Authors (2010). Journal compilation © New Phytologist Trust (2010).en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPHen_HK
dc.relation.ispartofNew Phytologisten_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectChloroplast translationen_HK
dc.subjectNOA1/RIF1en_HK
dc.subjectOs02g01440en_HK
dc.subjectPlant cGTPaseen_HK
dc.subjectRice (Oryza sativa)en_HK
dc.subject.meshArabidopsis - enzymology-
dc.subject.meshChloroplasts - enzymology-
dc.subject.meshConserved Sequence - genetics-
dc.subject.meshNitric Oxide Synthase - chemistry-
dc.subject.meshPlant Proteins - chemistry - genetics - metabolism-
dc.titleOsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: Implication for a highly conserved plant cGTPase essential for chloroplast functionen_HK
dc.typeArticleen_HK
dc.identifier.emailChu, IK: ivankchu@hku.hken_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1469-8137.2010.03264.xen_HK
dc.identifier.pmid20456051en_HK
dc.identifier.scopuseid_2-s2.0-77954588661en_HK
dc.identifier.hkuros170887en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954588661&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume187en_HK
dc.identifier.issue1en_HK
dc.identifier.spage83en_HK
dc.identifier.epage105en_HK
dc.identifier.eissn1469-8137-
dc.identifier.isiWOS:000278395100011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, H=36170607300en_HK
dc.identifier.scopusauthoridLau, E=35302963200en_HK
dc.identifier.scopusauthoridLam, MPY=35302594800en_HK
dc.identifier.scopusauthoridChu, H=36870371300en_HK
dc.identifier.scopusauthoridLi, S=16230452800en_HK
dc.identifier.scopusauthoridHuang, G=17434852200en_HK
dc.identifier.scopusauthoridGuo, P=34770450500en_HK
dc.identifier.scopusauthoridWang, J=13102773900en_HK
dc.identifier.scopusauthoridJiang, L=7403475889en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK
dc.identifier.scopusauthoridTao, Y=35786636800en_HK

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