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Article: Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae

TitleCombined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae
Authors
Issue Date2011
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm
Citation
Molecular Biosystems, 2011, v. 7 n. 7, p. 2128-2138 How to Cite?
AbstractFlavonoids isolated from Herba Epimedii such as icaritin, icariin and epimedin C have been suggested as potential bone anabolic compounds. However, the "specific localized effects" of these flavonoids in bone, in vivo, and the metabolism of these flavonoids in zebrafish larvae have never been demonstrated. In this study, we used multiple methods including in vivo imaging, drug metabolites profiling, transcriptomic and proteomic approaches to determine the mechanisms involved in the distribution and metabolism of the flavonoids in zebrafish larvae by measuring the fluorescence emission, in vivo, of icaritin and its glycoside derivatives. The fluorescence emission mechanism of icaritin in vitro was identified by spectrophotometric analysis, and the fluorescent property of icaritin was used as a probe to visualize the metabolism and distribution of icaritin and its glycoside derivatives in zebrafish larvae. Phase I and phase II metabolism of icaritin and its derivatives were identified in zebrafish by mass spectrometry. The combined transcriptomics and proteomics demonstrate a high degree of conservation of phase I and phase II drug metabolic enzymes between zebrafish larvae and mammals. Icaritin and its glycoside derivatives were demonstrated using combined approaches of in vivo imaging, drug metabolites identification, and transcriptomic and proteomic profiling to illustrate phase I and phase II metabolism of the flavonoids and their distribution in bone of zebrafish larvae. This study provides a new methodological model for use of the zebrafish larvae to examine drug metabolism. © 2011 The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/135022
ISSN
2015 Impact Factor: 2.829
2015 SCImago Journal Rankings: 1.238
ISI Accession Number ID
Funding AgencyGrant Number
Macau SAR045/2007
058/2009
Research Committee, University of MacauUL017
A-STAR, Singapore07/1/21/19/544
Funding Information:

This study is supported by a grant from the Science and Technology Development Fund of Macau SAR (Ref. No. 045/2007 and Ref. No. 058/2009) and Research Committee, University of Macau (UL017) and BMRC grant from A-STAR, Singapore (grant number 07/1/21/19/544). The authors state no conflict of interest.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, ZHen_HK
dc.contributor.authorAlex, Den_HK
dc.contributor.authorSiu, SOen_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorRenn, Jen_HK
dc.contributor.authorWinkler, Cen_HK
dc.contributor.authorLou, Sen_HK
dc.contributor.authorTsui, SKWen_HK
dc.contributor.authorZhao, HYen_HK
dc.contributor.authorYan, WRen_HK
dc.contributor.authorMahady, GBen_HK
dc.contributor.authorLi, GHen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorWang, YTen_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2011-07-27T01:26:12Z-
dc.date.available2011-07-27T01:26:12Z-
dc.date.issued2011en_HK
dc.identifier.citationMolecular Biosystems, 2011, v. 7 n. 7, p. 2128-2138en_HK
dc.identifier.issn1742-206Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/135022-
dc.description.abstractFlavonoids isolated from Herba Epimedii such as icaritin, icariin and epimedin C have been suggested as potential bone anabolic compounds. However, the "specific localized effects" of these flavonoids in bone, in vivo, and the metabolism of these flavonoids in zebrafish larvae have never been demonstrated. In this study, we used multiple methods including in vivo imaging, drug metabolites profiling, transcriptomic and proteomic approaches to determine the mechanisms involved in the distribution and metabolism of the flavonoids in zebrafish larvae by measuring the fluorescence emission, in vivo, of icaritin and its glycoside derivatives. The fluorescence emission mechanism of icaritin in vitro was identified by spectrophotometric analysis, and the fluorescent property of icaritin was used as a probe to visualize the metabolism and distribution of icaritin and its glycoside derivatives in zebrafish larvae. Phase I and phase II metabolism of icaritin and its derivatives were identified in zebrafish by mass spectrometry. The combined transcriptomics and proteomics demonstrate a high degree of conservation of phase I and phase II drug metabolic enzymes between zebrafish larvae and mammals. Icaritin and its glycoside derivatives were demonstrated using combined approaches of in vivo imaging, drug metabolites identification, and transcriptomic and proteomic profiling to illustrate phase I and phase II metabolism of the flavonoids and their distribution in bone of zebrafish larvae. This study provides a new methodological model for use of the zebrafish larvae to examine drug metabolism. © 2011 The Royal Society of Chemistry.en_HK
dc.languageengen_US
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htmen_HK
dc.relation.ispartofMolecular BioSystemsen_HK
dc.titleCombined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvaeen_HK
dc.typeArticleen_HK
dc.identifier.emailChu, IK:ivankchu@hku.hken_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/c1mb00001ben_HK
dc.identifier.pmid21445457-
dc.identifier.scopuseid_2-s2.0-79959215895en_HK
dc.identifier.hkuros186169en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959215895&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2128en_HK
dc.identifier.epage2138en_HK
dc.identifier.isiWOS:000291559900005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, ZH=54401253000en_HK
dc.identifier.scopusauthoridAlex, D=8270038300en_HK
dc.identifier.scopusauthoridSiu, SO=8603087200en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridRenn, J=7004546973en_HK
dc.identifier.scopusauthoridWinkler, C=7102748741en_HK
dc.identifier.scopusauthoridLou, S=36182669800en_HK
dc.identifier.scopusauthoridTsui, SKW=7004961364en_HK
dc.identifier.scopusauthoridZhao, HY=54402036500en_HK
dc.identifier.scopusauthoridYan, WR=54401871700en_HK
dc.identifier.scopusauthoridMahady, GB=55212597700en_HK
dc.identifier.scopusauthoridLi, GH=40462073700en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridWang, YT=23979017200en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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