File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
  • Basic View
  • Metadata View
  • XML View
TitleEffects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
 
AuthorsChin, A1
Yang, Y1
Chai, L1
Wong, RWK1
Rabie, ABM1
 
Keywordsosteoblasts
Salvia Miltiorrhiza
 
Issue Date2011
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres
 
CitationJournal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jor.21376
 
AbstractSufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society.
 
ISSN0736-0266
2013 Impact Factor: 2.972
2013 SCImago Journal Rankings: 1.505
 
DOIhttp://dx.doi.org/10.1002/jor.21376
 
ISI Accession Number IDWOS:000290632900014
Funding AgencyGrant Number
HKU201007176194
2010071761494
Funding Information:

We thank Mr. Shadow Yeung and Mr. Raymond Tong for their technical assistance. This work is supported by Small Project Funding (2010071761494), HKU (201007176194).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChin, A
 
dc.contributor.authorYang, Y
 
dc.contributor.authorChai, L
 
dc.contributor.authorWong, RWK
 
dc.contributor.authorRabie, ABM
 
dc.date.accessioned2011-07-27T01:25:32Z
 
dc.date.available2011-07-27T01:25:32Z
 
dc.date.issued2011
 
dc.description.abstractSufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jor.21376
 
dc.identifier.doihttp://dx.doi.org/10.1002/jor.21376
 
dc.identifier.eissn1554-527X
 
dc.identifier.epage1063
 
dc.identifier.hkuros188897
 
dc.identifier.isiWOS:000290632900014
Funding AgencyGrant Number
HKU201007176194
2010071761494
Funding Information:

We thank Mr. Shadow Yeung and Mr. Raymond Tong for their technical assistance. This work is supported by Small Project Funding (2010071761494), HKU (201007176194).

 
dc.identifier.issn0736-0266
2013 Impact Factor: 2.972
2013 SCImago Journal Rankings: 1.505
 
dc.identifier.issue7
 
dc.identifier.pmid21319217
 
dc.identifier.scopuseid_2-s2.0-79955895930
 
dc.identifier.spage1059
 
dc.identifier.urihttp://hdl.handle.net/10722/134997
 
dc.identifier.volume29
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Orthopaedic Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Orthopaedic Research. Copyright © John Wiley & Sons, Inc.
 
dc.subject.meshSalvia miltiorrhiza
 
dc.subject.meshRANK Ligand - genetics
 
dc.subject.meshPlant Preparations - pharmacology
 
dc.subject.meshOsteoprotegerin - genetics
 
dc.subject.meshOsteogenesis - drug effects
 
dc.subject.meshOsteocalcin - genetics
 
dc.subject.meshOsteoblasts - cytology - drug effects
 
dc.subject.meshMice
 
dc.subject.meshMedicine, Chinese Traditional - methods
 
dc.subject.meshMedicine, Chinese Traditional - methods
 
dc.subject.meshOsteoblasts - cytology - drug effects
 
dc.subject.meshOsteogenesis - drug effects
 
dc.subject.meshPlant Preparations - pharmacology
 
dc.subject.meshSalvia miltiorrhiza
 
dc.subjectosteoblasts
 
dc.subjectSalvia Miltiorrhiza
 
dc.titleEffects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Chin, A</contributor.author>
<contributor.author>Yang, Y</contributor.author>
<contributor.author>Chai, L</contributor.author>
<contributor.author>Wong, RWK</contributor.author>
<contributor.author>Rabie, ABM</contributor.author>
<date.accessioned>2011-07-27T01:25:32Z</date.accessioned>
<date.available>2011-07-27T01:25:32Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>Journal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063</identifier.citation>
<identifier.issn>0736-0266</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/134997</identifier.uri>
<description.abstract>Sufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p &lt; 0.001) and at 48 h by 13% (p &lt; 0.001). OCN was decreased by 22% at 24 h (p &lt; 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p &lt; 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p &lt; 0.001) and 36% (p &lt; 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p &lt; 0.01) and 68% at 72 h (p &lt; 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. &#169; 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright &#169; 2011 Orthopaedic Research Society.</description.abstract>
<language>eng</language>
<publisher>John Wiley &amp; Sons, Inc. The Journal&apos;s web site is located at http://www.elsevier.com/locate/orthres</publisher>
<relation.ispartof>Journal of Orthopaedic Research</relation.ispartof>
<rights>Journal of Orthopaedic Research. Copyright &#169; John Wiley &amp; Sons, Inc.</rights>
<subject>osteoblasts</subject>
<subject>Salvia Miltiorrhiza</subject>
<subject.mesh>Salvia miltiorrhiza</subject.mesh>
<subject.mesh>RANK Ligand - genetics</subject.mesh>
<subject.mesh>Plant Preparations - pharmacology</subject.mesh>
<subject.mesh>Osteoprotegerin - genetics</subject.mesh>
<subject.mesh>Osteogenesis - drug effects</subject.mesh>
<subject.mesh>Osteocalcin - genetics</subject.mesh>
<subject.mesh>Osteoblasts - cytology - drug effects</subject.mesh>
<subject.mesh>Mice</subject.mesh>
<subject.mesh>Medicine, Chinese Traditional - methods</subject.mesh>
<subject.mesh>Medicine, Chinese Traditional - methods</subject.mesh>
<subject.mesh>Osteoblasts - cytology - drug effects</subject.mesh>
<subject.mesh>Osteogenesis - drug effects</subject.mesh>
<subject.mesh>Plant Preparations - pharmacology</subject.mesh>
<subject.mesh>Salvia miltiorrhiza</subject.mesh>
<title>Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro</title>
<type>Article</type>
<description.nature>link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1002/jor.21376</identifier.doi>
<identifier.pmid>21319217</identifier.pmid>
<identifier.scopus>eid_2-s2.0-79955895930</identifier.scopus>
<identifier.hkuros>188897</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-79955895930&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>29</identifier.volume>
<identifier.issue>7</identifier.issue>
<identifier.spage>1059</identifier.spage>
<identifier.epage>1063</identifier.epage>
<identifier.eissn>1554-527X</identifier.eissn>
<identifier.isi>WOS:000290632900014</identifier.isi>
<publisher.place>United States</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong