Article: Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
| Title | Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Chin, A1 Yang, Y1 Chai, L1 Wong, RWK1 Rabie, ABM1 | ||||||
| Keywords | osteoblasts Salvia Miltiorrhiza | ||||||
| Issue Date | 2011 | ||||||
| Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres | ||||||
| Citation | Journal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063 [How to Cite?] DOI: http://dx.doi.org/10.1002/jor.21376 | ||||||
| Abstract | Sufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society. | ||||||
| ISSN | 0736-0266 2011 Impact Factor: 2.811 2011 SCImago Journal Rankings: 0.210 | ||||||
| DOI | http://dx.doi.org/10.1002/jor.21376 | ||||||
| ISI Accession Number ID | WOS:000290632900014
Funding Information: We thank Mr. Shadow Yeung and Mr. Raymond Tong for their technical assistance. This work is supported by Small Project Funding (2010071761494), HKU (201007176194). | ||||||
| References | References in Scopus | ||||||
| Grants | Effects of medicinal herb Danshen on bone cells activity in vitro |
| dc.contributor.author | Chin, A | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Yang, Y | ||||||
| dc.contributor.author | Chai, L | ||||||
| dc.contributor.author | Wong, RWK | ||||||
| dc.contributor.author | Rabie, ABM | ||||||
| dc.date.accessioned | 2011-07-27T01:25:32Z | ||||||
| dc.date.available | 2011-07-27T01:25:32Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | Sufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society. | ||||||
| dc.description.grant | Effects of medicinal herb Danshen on bone cells activity in vitro | ||||||
| dc.description.grantcode | 103877 | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063 [How to Cite?] DOI: http://dx.doi.org/10.1002/jor.21376 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1002/jor.21376 | ||||||
| dc.identifier.epage | 1063 | ||||||
| dc.identifier.hkuros | 188897 | ||||||
| dc.identifier.isi | WOS:000290632900014
Funding Information: We thank Mr. Shadow Yeung and Mr. Raymond Tong for their technical assistance. This work is supported by Small Project Funding (2010071761494), HKU (201007176194). | ||||||
| dc.identifier.issn | 0736-0266 2011 Impact Factor: 2.811 2011 SCImago Journal Rankings: 0.210 | ||||||
| dc.identifier.issue | 7 | ||||||
| dc.identifier.pmid | 21319217 | ||||||
| dc.identifier.scopus | eid_2-s2.0-79955895930 | ||||||
| dc.identifier.spage | 1059 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/134997 | ||||||
| dc.identifier.volume | 29 | ||||||
| dc.language | eng | ||||||
| dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Journal of Orthopaedic Research | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | Journal of Orthopaedic Research. Copyright © John Wiley & Sons, Inc. | ||||||
| dc.subject.mesh | Salvia miltiorrhiza | ||||||
| dc.subject.mesh | RANK Ligand - genetics | ||||||
| dc.subject.mesh | Plant Preparations - pharmacology | ||||||
| dc.subject.mesh | Osteoprotegerin - genetics | ||||||
| dc.subject.mesh | Osteogenesis - drug effects | ||||||
| dc.subject.mesh | Osteocalcin - genetics | ||||||
| dc.subject.mesh | Osteoblasts - cytology - drug effects | ||||||
| dc.subject.mesh | Mice | ||||||
| dc.subject.mesh | Medicine, Chinese Traditional - methods | ||||||
| dc.subject.mesh | Medicine, Chinese Traditional - methods | ||||||
| dc.subject.mesh | Osteoblasts - cytology - drug effects | ||||||
| dc.subject.mesh | Osteogenesis - drug effects | ||||||
| dc.subject.mesh | Plant Preparations - pharmacology | ||||||
| dc.subject.mesh | Salvia miltiorrhiza | ||||||
| dc.subject | osteoblasts | ||||||
| dc.subject | Salvia Miltiorrhiza | ||||||
| dc.title | Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong