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Article: Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro

TitleEffects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro
Authors
Keywordsosteoblasts
Salvia Miltiorrhiza
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres
Citation
Journal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063 How to Cite?
Abstract
Sufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society.
Persistent Identifierhttp://hdl.handle.net/10722/134997
ISSN
2013 Impact Factor: 2.972
2013 SCImago Journal Rankings: 1.505
ISI Accession Number ID
Funding AgencyGrant Number
HKU201007176194
2010071761494
Funding Information:

We thank Mr. Shadow Yeung and Mr. Raymond Tong for their technical assistance. This work is supported by Small Project Funding (2010071761494), HKU (201007176194).

References

 

DC FieldValueLanguage
dc.contributor.authorChin, Aen_HK
dc.contributor.authorYang, Yen_HK
dc.contributor.authorChai, Len_HK
dc.contributor.authorWong, RWKen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2011-07-27T01:25:32Z-
dc.date.available2011-07-27T01:25:32Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Orthopaedic Research, 2011, v. 29 n. 7, p. 1059-1063en_HK
dc.identifier.issn0736-0266en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134997-
dc.description.abstractSufficient osteoinduction is essential for the success and effectiveness of bone grafting. It was previously found that Salvia Miltiorrhiza (SM), a commonly used Chinese herb increased osteogenesis in vivo. The aim of this study is to investigate the effects of SM on bone cells in vitro, in an attempt to get a better understanding on how SM can promote bone remodeling. MC3T3-E1, an osteoblastic cell line, was cultured with SM for different time intervals (24, 48, and 72 h), whereas the control group consisted of cells cultured without any intervention. The mRNA expression of alkaline phosphatase (ALP), osteocalcin (OCN), osteoprotegerin (OPG), and the receptor activator of nuclear factor kappa B ligand (RANKL) were examined by real-time polymerase chain reaction (qPCR). The expression of ALP showed an early increase at 24 h by 50% (p < 0.001) and at 48 h by 13% (p < 0.001). OCN was decreased by 22% at 24 h (p < 0.001) but increased by 50% and 88% at 48 and 72 h, respectively (p < 0.001). RANKL showed an early increase at the first two time points of 24 and 48 h by 45% (p < 0.001) and 36% (p < 0.01), respectively, while OPG was up-regulated at the latter two time points by 10% at 48 h (p < 0.01) and 68% at 72 h (p < 0.001). Thus, OPG/RANKL was down-regulated first, and then up-regulated. SM enhances bone remodeling by regulating the gene expression of ALP, OCN, OPG, and RANKL. It is a potential medicinal herb to be utilized in the application that requires stimulation in bone cell activities. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1059-1063, 2011 Copyright © 2011 Orthopaedic Research Society.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthresen_HK
dc.relation.ispartofJournal of Orthopaedic Researchen_HK
dc.rightsJournal of Orthopaedic Research. Copyright © John Wiley & Sons, Inc.-
dc.subjectosteoblastsen_HK
dc.subjectSalvia Miltiorrhizaen_HK
dc.subject.meshSalvia miltiorrhizaen_HK
dc.subject.meshRANK Ligand - geneticsen_HK
dc.subject.meshPlant Preparations - pharmacologyen_HK
dc.subject.meshOsteoprotegerin - geneticsen_HK
dc.subject.meshOsteogenesis - drug effectsen_HK
dc.subject.meshOsteocalcin - geneticsen_HK
dc.subject.meshOsteoblasts - cytology - drug effectsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMedicine, Chinese Traditional - methodsen_HK
dc.subject.meshMedicine, Chinese Traditional - methodsen_HK
dc.subject.meshOsteoblasts - cytology - drug effectsen_HK
dc.subject.meshOsteogenesis - drug effectsen_HK
dc.subject.meshPlant Preparations - pharmacologyen_HK
dc.subject.meshSalvia miltiorrhizaen_HK
dc.titleEffects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailYang, Y: yangyanq@hkucc.hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityYang, Y=rp00045en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jor.21376en_HK
dc.identifier.pmid21319217en_HK
dc.identifier.scopuseid_2-s2.0-79955895930en_HK
dc.identifier.hkuros188897en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955895930&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1059en_HK
dc.identifier.epage1063en_HK
dc.identifier.eissn1554-527X-
dc.identifier.isiWOS:000290632900014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChin, A=47461053100en_HK
dc.identifier.scopusauthoridYang, Y=36623085300en_HK
dc.identifier.scopusauthoridChai, L=36196100600en_HK
dc.identifier.scopusauthoridWong, RWK=36642905800en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK

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