File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: B7-H3 and 4-1BBL cooperatively enhance the antitumor response of CD8 T cells in oral cancer
  • Basic View
  • Metadata View
  • XML View
TitleB7-H3 and 4-1BBL cooperatively enhance the antitumor response of CD8 T cells in oral cancer
 
AuthorsYu, S2 4
Tang, J4
Wang, F4
Huang, H3
Wang, H4
Ma, P4
Zheng, L1
Zwahlen, RA1
Yang, H4
 
Keywords4-1BBL
B7-H3
CD8T cell
Primary oral cancer cell
 
Issue Date2011
 
PublisherAmerican Scientific Publishers. The Journal's web site is located at http://www.aspbs.com/science/
 
CitationAdvanced Science Letters, 2011, v. 4 n. 2, p. 357-362 [How to Cite?]
DOI: http://dx.doi.org/10.1166/asl.2011.1234
 
AbstractThe aim of this study was to enhance the antitumor immunity of CD8 T cells directed against human oral cancer using B7-H3 and 4-1BBL gene transfer. Recombinant replication-defective adenovirus 5 (Adv) expressing human 4-1BBL, B7-H3 or 4-1BBL/B7-H3 was constructed using the two-plasmid rescue method. Primary human oral cancer cell vaccines (OCV) were prepared by treating cells with 20 μg/ml mitomycin-C after primary human oral cancer cells were infected with Adv transduced with 4-1BBL, B7-H3, 4-1BBL/B7-H3 or control Adv. Then, autologous, purified CD8 T cells were stimulated by coculture with OCV for 4 days. At the end of the culture, CD8 T cell activation was evaluated by ELISA to assay the production of IFNγ and IL-2 in the culture supernatants. Cytotoxic CD8 T cell effector function was analyzed by a 51Chromium (Cr)-release assay, and CD8 T cell proliferation was evaluated by flow cytometry and Cell Counting Kit-8 (CCK-8) assays. Primary human oral cancer cells expressed low levels of B7-H3 and 4-1BBL and weakly stimulated autologous T cells. The primary human oral cancer cell vaccine transduced with the combination of 4-1BBL with B7-H3 retroviruses resulted in significantly enhanced CD8 T cell activation and proliferation. This was associated with increased IFNγ and IL-2 production by CD8 T cells and improved CD8 T cell proliferation. CD8 T cells stimulated with the autologous B7-3/4-1BBL primary human oral cancer cell vaccine efficiently killed the autologous parental B7-H3/4-1BBL primary human oral cancer cells. Strong CD8 T cell activation, proliferation and longer survival time could be obtained by this vaccine. These data provide a mechanism for developing tumor vaccines using modified tumor cells in patients with oral cancer. © 2011 American Scientific Publishers.
 
ISSN1936-6612
 
DOIhttp://dx.doi.org/10.1166/asl.2011.1234
 
ISI Accession Number IDWOS:000291410900013
Funding AgencyGrant Number
National Natural Science Foundation of China30973339
Guangdong Province Natural Science Foundation104518036002006303
Guangdong Province Science and Technology Agency2010B050100007
foundation of Shenzhen Bureau of Science Technology and InformationJC200903180665A
Funding Information:

This work was supported by the National Natural Science Foundation of China (grant no. 30973339), the Guangdong Province Natural Science Foundation (grant no. 104518036002006303), the Guangdong Province Science and Technology Agency (grant no. 2010B050100007) and the foundation of Shenzhen Bureau of Science Technology and Information (grant no. JC200903180665A).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYu, S
 
dc.contributor.authorTang, J
 
dc.contributor.authorWang, F
 
dc.contributor.authorHuang, H
 
dc.contributor.authorWang, H
 
dc.contributor.authorMa, P
 
dc.contributor.authorZheng, L
 
dc.contributor.authorZwahlen, RA
 
dc.contributor.authorYang, H
 
dc.date.accessioned2011-07-27T01:25:25Z
 
dc.date.available2011-07-27T01:25:25Z
 
dc.date.issued2011
 
dc.description.abstractThe aim of this study was to enhance the antitumor immunity of CD8 T cells directed against human oral cancer using B7-H3 and 4-1BBL gene transfer. Recombinant replication-defective adenovirus 5 (Adv) expressing human 4-1BBL, B7-H3 or 4-1BBL/B7-H3 was constructed using the two-plasmid rescue method. Primary human oral cancer cell vaccines (OCV) were prepared by treating cells with 20 μg/ml mitomycin-C after primary human oral cancer cells were infected with Adv transduced with 4-1BBL, B7-H3, 4-1BBL/B7-H3 or control Adv. Then, autologous, purified CD8 T cells were stimulated by coculture with OCV for 4 days. At the end of the culture, CD8 T cell activation was evaluated by ELISA to assay the production of IFNγ and IL-2 in the culture supernatants. Cytotoxic CD8 T cell effector function was analyzed by a 51Chromium (Cr)-release assay, and CD8 T cell proliferation was evaluated by flow cytometry and Cell Counting Kit-8 (CCK-8) assays. Primary human oral cancer cells expressed low levels of B7-H3 and 4-1BBL and weakly stimulated autologous T cells. The primary human oral cancer cell vaccine transduced with the combination of 4-1BBL with B7-H3 retroviruses resulted in significantly enhanced CD8 T cell activation and proliferation. This was associated with increased IFNγ and IL-2 production by CD8 T cells and improved CD8 T cell proliferation. CD8 T cells stimulated with the autologous B7-3/4-1BBL primary human oral cancer cell vaccine efficiently killed the autologous parental B7-H3/4-1BBL primary human oral cancer cells. Strong CD8 T cell activation, proliferation and longer survival time could be obtained by this vaccine. These data provide a mechanism for developing tumor vaccines using modified tumor cells in patients with oral cancer. © 2011 American Scientific Publishers.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAdvanced Science Letters, 2011, v. 4 n. 2, p. 357-362 [How to Cite?]
DOI: http://dx.doi.org/10.1166/asl.2011.1234
 
dc.identifier.doihttp://dx.doi.org/10.1166/asl.2011.1234
 
dc.identifier.epage362
 
dc.identifier.hkuros188024
 
dc.identifier.isiWOS:000291410900013
Funding AgencyGrant Number
National Natural Science Foundation of China30973339
Guangdong Province Natural Science Foundation104518036002006303
Guangdong Province Science and Technology Agency2010B050100007
foundation of Shenzhen Bureau of Science Technology and InformationJC200903180665A
Funding Information:

This work was supported by the National Natural Science Foundation of China (grant no. 30973339), the Guangdong Province Natural Science Foundation (grant no. 104518036002006303), the Guangdong Province Science and Technology Agency (grant no. 2010B050100007) and the foundation of Shenzhen Bureau of Science Technology and Information (grant no. JC200903180665A).

 
dc.identifier.issn1936-6612
 
dc.identifier.issue2
 
dc.identifier.scopuseid_2-s2.0-79953109589
 
dc.identifier.spage357
 
dc.identifier.urihttp://hdl.handle.net/10722/134987
 
dc.identifier.volume4
 
dc.languageeng
 
dc.publisherAmerican Scientific Publishers. The Journal's web site is located at http://www.aspbs.com/science/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAdvanced Science Letters
 
dc.relation.referencesReferences in Scopus
 
dc.subject4-1BBL
 
dc.subjectB7-H3
 
dc.subjectCD8T cell
 
dc.subjectPrimary oral cancer cell
 
dc.titleB7-H3 and 4-1BBL cooperatively enhance the antitumor response of CD8 T cells in oral cancer
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Yu, S</contributor.author>
<contributor.author>Tang, J</contributor.author>
<contributor.author>Wang, F</contributor.author>
<contributor.author>Huang, H</contributor.author>
<contributor.author>Wang, H</contributor.author>
<contributor.author>Ma, P</contributor.author>
<contributor.author>Zheng, L</contributor.author>
<contributor.author>Zwahlen, RA</contributor.author>
<contributor.author>Yang, H</contributor.author>
<date.accessioned>2011-07-27T01:25:25Z</date.accessioned>
<date.available>2011-07-27T01:25:25Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>Advanced Science Letters, 2011, v. 4 n. 2, p. 357-362</identifier.citation>
<identifier.issn>1936-6612</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/134987</identifier.uri>
<description.abstract>The aim of this study was to enhance the antitumor immunity of CD8 T cells directed against human oral cancer using B7-H3 and 4-1BBL gene transfer. Recombinant replication-defective adenovirus 5 (Adv) expressing human 4-1BBL, B7-H3 or 4-1BBL/B7-H3 was constructed using the two-plasmid rescue method. Primary human oral cancer cell vaccines (OCV) were prepared by treating cells with 20 &#956;g/ml mitomycin-C after primary human oral cancer cells were infected with Adv transduced with 4-1BBL, B7-H3, 4-1BBL/B7-H3 or control Adv. Then, autologous, purified CD8 T cells were stimulated by coculture with OCV for 4 days. At the end of the culture, CD8 T cell activation was evaluated by ELISA to assay the production of IFN&#947; and IL-2 in the culture supernatants. Cytotoxic CD8 T cell effector function was analyzed by a 51Chromium (Cr)-release assay, and CD8 T cell proliferation was evaluated by flow cytometry and Cell Counting Kit-8 (CCK-8) assays. Primary human oral cancer cells expressed low levels of B7-H3 and 4-1BBL and weakly stimulated autologous T cells. The primary human oral cancer cell vaccine transduced with the combination of 4-1BBL with B7-H3 retroviruses resulted in significantly enhanced CD8 T cell activation and proliferation. This was associated with increased IFN&#947; and IL-2 production by CD8 T cells and improved CD8 T cell proliferation. CD8 T cells stimulated with the autologous B7-3/4-1BBL primary human oral cancer cell vaccine efficiently killed the autologous parental B7-H3/4-1BBL primary human oral cancer cells. Strong CD8 T cell activation, proliferation and longer survival time could be obtained by this vaccine. These data provide a mechanism for developing tumor vaccines using modified tumor cells in patients with oral cancer. &#169; 2011 American Scientific Publishers.</description.abstract>
<language>eng</language>
<publisher>American Scientific Publishers. The Journal&apos;s web site is located at http://www.aspbs.com/science/</publisher>
<relation.ispartof>Advanced Science Letters</relation.ispartof>
<subject>4-1BBL</subject>
<subject>B7-H3</subject>
<subject>CD8T cell</subject>
<subject>Primary oral cancer cell</subject>
<title>B7-H3 and 4-1BBL cooperatively enhance the antitumor response of CD8 T cells in oral cancer</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1166/asl.2011.1234</identifier.doi>
<identifier.scopus>eid_2-s2.0-79953109589</identifier.scopus>
<identifier.hkuros>188024</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-79953109589&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>4</identifier.volume>
<identifier.issue>2</identifier.issue>
<identifier.spage>357</identifier.spage>
<identifier.epage>362</identifier.epage>
<identifier.isi>WOS:000291410900013</identifier.isi>
<publisher.place>United States</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong
  2. Shenzhen-PKU-HKUST Medical Center
  3. Sun Yat-Sen University
  4. Peking University