Article: LMO2 promotes angiogenesis probably by up-regulation of bFGF in endothelial cells: An implication of its pathophysiological role in infantile haemangioma
| Title | LMO2 promotes angiogenesis probably by up-regulation of bFGF in endothelial cells: An implication of its pathophysiological role in infantile haemangioma | ||||
|---|---|---|---|---|---|
| Authors | Sun, ZJ2 3 Cai, Y2 3 Chen, G2 3 Wang, R3 Jia, J2 3 Chen, XM2 Zheng, LW1 Zhao, YF2 3 | ||||
| Keywords | BFGF Endothelial cells Infantile haemangiomas LMO2 | ||||
| Issue Date | 2010 | ||||
| Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS | ||||
| Citation | Histopathology, 2010, v. 57 n. 4, p. 622-632 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1365-2559.2010.03676.x | ||||
| Abstract | Aims: Infantile haemangiomas (IHs) are common benign vascular tumours distinctive for their perinatal presentation, rapid growth during the first year of life and subsequent slow involution. Recent research has indicated that endothelial cells of haemangiomas express LIM-only protein 2 (LMO2). The aim of this study was to investigate the role of LMO2 in the pathogenesis of IHs was investigated.Methods and results: Immunoreactivity of LMO2 was assessed in specimens of 19 IH. Stable transfection of LMO2 into human endothelial cell lines (EAhy926) was performed to evaluate the role of LMO2 in terms of the change in cell proliferation, cell cycle and cell migration as well as the expression level of angiogenic factors. Immunoreactivity for LMO2 was detected in all IH specimens, specifically in the nucleus of the endothelial cells. The intensity of LMO2 immunostaining decreased significantly from proliferative to involuting stages. Furthermore, the overexpression of LMO2 enhanced the proliferation and migration of the endothelial cells and promoted G0/G1-S-phase transition in vitro, together with an up-regulation of bFGF expression.Conclusions: LMO2 probably promotes angiogenesis by up-regulation of bFGF expression and thereby consequently influences progression of IH. © 2010 Blackwell Publishing Limited. | ||||
| ISSN | 0309-0167 2011 Impact Factor: 3.082 2011 SCImago Journal Rankings: 0.320 | ||||
| DOI | http://dx.doi.org/10.1111/j.1365-2559.2010.03676.x | ||||
| ISI Accession Number ID | WOS:000283078300015
Funding Information: This work was supported by grants from the National Natural Science Foundation of China (30600712) to Dr Z J Sun and (30872894 and 30973330) to Dr Y F Zhao. We thank Dr Roger A Zwahlen of The Faculty of Dentistry, The University of Hong Kong for his kind assistance in editing. | ||||
| References | References in Scopus |
| dc.contributor.author | Sun, ZJ | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Cai, Y | ||||
| dc.contributor.author | Chen, G | ||||
| dc.contributor.author | Wang, R | ||||
| dc.contributor.author | Jia, J | ||||
| dc.contributor.author | Chen, XM | ||||
| dc.contributor.author | Zheng, LW | ||||
| dc.contributor.author | Zhao, YF | ||||
| dc.date.accessioned | 2011-07-27T01:25:24Z | ||||
| dc.date.available | 2011-07-27T01:25:24Z | ||||
| dc.date.issued | 2010 | ||||
| dc.description.abstract | Aims: Infantile haemangiomas (IHs) are common benign vascular tumours distinctive for their perinatal presentation, rapid growth during the first year of life and subsequent slow involution. Recent research has indicated that endothelial cells of haemangiomas express LIM-only protein 2 (LMO2). The aim of this study was to investigate the role of LMO2 in the pathogenesis of IHs was investigated.Methods and results: Immunoreactivity of LMO2 was assessed in specimens of 19 IH. Stable transfection of LMO2 into human endothelial cell lines (EAhy926) was performed to evaluate the role of LMO2 in terms of the change in cell proliferation, cell cycle and cell migration as well as the expression level of angiogenic factors. Immunoreactivity for LMO2 was detected in all IH specimens, specifically in the nucleus of the endothelial cells. The intensity of LMO2 immunostaining decreased significantly from proliferative to involuting stages. Furthermore, the overexpression of LMO2 enhanced the proliferation and migration of the endothelial cells and promoted G0/G1-S-phase transition in vitro, together with an up-regulation of bFGF expression.Conclusions: LMO2 probably promotes angiogenesis by up-regulation of bFGF expression and thereby consequently influences progression of IH. © 2010 Blackwell Publishing Limited. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Histopathology, 2010, v. 57 n. 4, p. 622-632 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1365-2559.2010.03676.x | ||||
| dc.identifier.citeulike | 8049268 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1365-2559.2010.03676.x | ||||
| dc.identifier.epage | 632 | ||||
| dc.identifier.hkuros | 188022 | ||||
| dc.identifier.isi | WOS:000283078300015
Funding Information: This work was supported by grants from the National Natural Science Foundation of China (30600712) to Dr Z J Sun and (30872894 and 30973330) to Dr Y F Zhao. We thank Dr Roger A Zwahlen of The Faculty of Dentistry, The University of Hong Kong for his kind assistance in editing. | ||||
| dc.identifier.issn | 0309-0167 2011 Impact Factor: 3.082 2011 SCImago Journal Rankings: 0.320 | ||||
| dc.identifier.issue | 4 | ||||
| dc.identifier.pmid | 20955387 | ||||
| dc.identifier.scopus | eid_2-s2.0-78650504772 | ||||
| dc.identifier.spage | 622 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/134986 | ||||
| dc.identifier.volume | 57 | ||||
| dc.language | eng | ||||
| dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS | ||||
| dc.publisher.place | United Kingdom | ||||
| dc.relation.ispartof | Histopathology | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | The definitive version is available at www.blackwell-synergy.com | ||||
| dc.subject.mesh | DNA-Binding Proteins - metabolism | ||||
| dc.subject.mesh | Endothelial Cells - metabolism | ||||
| dc.subject.mesh | Fibroblast Growth Factor 2 - biosynthesis | ||||
| dc.subject.mesh | Hemangioma, Capillary - metabolism | ||||
| dc.subject.mesh | Metalloproteins - metabolism | ||||
| dc.subject | BFGF | ||||
| dc.subject | Endothelial cells | ||||
| dc.subject | Infantile haemangiomas | ||||
| dc.subject | LMO2 | ||||
| dc.title | LMO2 promotes angiogenesis probably by up-regulation of bFGF in endothelial cells: An implication of its pathophysiological role in infantile haemangioma | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Wuhan University
- State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)