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Article: Ultrastructure and morphology of biofilms on thermoplastic orthodontic appliances in 'fast' and 'slow' plaque formers

TitleUltrastructure and morphology of biofilms on thermoplastic orthodontic appliances in 'fast' and 'slow' plaque formers
Authors
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://ejo.oxfordjournals.org/
Citation
European Journal Of Orthodontics, 2011, v. 33 n. 5, p. 577-583 How to Cite?
AbstractThe aim of this study was to investigate the morphological features and distribution of biofilms on Invisalign orthodontic appliances, in a sample of 'slow' and 'fast' plaque formers using scanning electron microscopy (SEM). Fifty-six Chinese male/female volunteers (aged 19-39 years) were screened for their plaque-forming rate using the plaque percentage index (PPI) coupled with digital photography and computer-based image analysis, after a period of 48 hours of abstinence from oral hygiene procedures. Eleven volunteers (seven males/four females) representing the lowest and highest ends of the plaque formation spectrum were chosen as slow and fast plaque formers, respectively. The subjects wore a full-coverage splint appliance, in which four tiles of Invisalign material were embedded. These tiles were collected at intervals of 1, 3, 6, 12, 24, and 48 hours, as well as 3, 7, and 14 days, immediately fixed in 10 per cent paraformaldehyde in 0.2 M cacodylate buffer solution and prepared for SEM. The surface configuration of the Invisalign appliance was visualized, as well as the chronological pattern of biofilm formation. Significance between fast and slow plaque formers was determined using a Student's t-test.Colonization appeared to centre initially on the raised edges or textured surfaces of the appliance, and initial adhesion was quicker and more abundant in the fast plaque-forming group. In the later stages of biofilm development, both groups showed no discernible differences in biofilm accrual on the surfaces, but the fast group displayed a more complex biofilm structure. More recessed and sheltered areas of the appliance, such as the cusp tips and attachment dimples, harboured more biofilm than the flat surfaces. Hence, it seems that the novel Invisialign orthodontic appliance is a useful tool to investigate the features of biofilm formation in time-course studies. The Author 2010. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com2011 © The Author 2010. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Persistent Identifierhttp://hdl.handle.net/10722/134961
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLow, Ben_HK
dc.contributor.authorLee, Wen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorHägg, Uen_HK
dc.date.accessioned2011-07-27T01:25:08Z-
dc.date.available2011-07-27T01:25:08Z-
dc.date.issued2011en_HK
dc.identifier.citationEuropean Journal Of Orthodontics, 2011, v. 33 n. 5, p. 577-583en_HK
dc.identifier.issn0141-5387en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134961-
dc.description.abstractThe aim of this study was to investigate the morphological features and distribution of biofilms on Invisalign orthodontic appliances, in a sample of 'slow' and 'fast' plaque formers using scanning electron microscopy (SEM). Fifty-six Chinese male/female volunteers (aged 19-39 years) were screened for their plaque-forming rate using the plaque percentage index (PPI) coupled with digital photography and computer-based image analysis, after a period of 48 hours of abstinence from oral hygiene procedures. Eleven volunteers (seven males/four females) representing the lowest and highest ends of the plaque formation spectrum were chosen as slow and fast plaque formers, respectively. The subjects wore a full-coverage splint appliance, in which four tiles of Invisalign material were embedded. These tiles were collected at intervals of 1, 3, 6, 12, 24, and 48 hours, as well as 3, 7, and 14 days, immediately fixed in 10 per cent paraformaldehyde in 0.2 M cacodylate buffer solution and prepared for SEM. The surface configuration of the Invisalign appliance was visualized, as well as the chronological pattern of biofilm formation. Significance between fast and slow plaque formers was determined using a Student's t-test.Colonization appeared to centre initially on the raised edges or textured surfaces of the appliance, and initial adhesion was quicker and more abundant in the fast plaque-forming group. In the later stages of biofilm development, both groups showed no discernible differences in biofilm accrual on the surfaces, but the fast group displayed a more complex biofilm structure. More recessed and sheltered areas of the appliance, such as the cusp tips and attachment dimples, harboured more biofilm than the flat surfaces. Hence, it seems that the novel Invisialign orthodontic appliance is a useful tool to investigate the features of biofilm formation in time-course studies. The Author 2010. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com2011 © The Author 2010. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://ejo.oxfordjournals.org/en_HK
dc.relation.ispartofEuropean Journal of Orthodonticsen_HK
dc.titleUltrastructure and morphology of biofilms on thermoplastic orthodontic appliances in 'fast' and 'slow' plaque formersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-5387&volume=33&issue=5&spage=577&epage=583&date=2010&atitle=Ultrastructure+and+morphology+of+biofilms+on+thermoplastic+orthodontic+appliances+in+%27fast%27+and+%27slow%27+plaque+formers-
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailHägg, U:euohagg@hkusua.hku.hken_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityHägg, U=rp00020en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjq126en_HK
dc.identifier.pmid21187528-
dc.identifier.scopuseid_2-s2.0-80053464335en_HK
dc.identifier.hkuros186255en_US
dc.identifier.hkuros191873-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053464335&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue5en_HK
dc.identifier.spage577en_HK
dc.identifier.epage583en_HK
dc.identifier.isiWOS:000295414500019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLow, B=24166942600en_HK
dc.identifier.scopusauthoridLee, W=35191948000en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridHägg, U=7006790279en_HK

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